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3462 (NS)
!3462NSIstYearMicrobiology! £vÄ Gs
Register Number
PART - III
~sq°›¯À / MICROBIOLOGY
( uªÌ ©ØÖ® B[Q» ÁÈ / Tamil & English Version)
Põ» AÍÄ : 3.00 ©o ÷|µ® ] [ ö©õzu ©v¨ö£sPÒ : 70
Time Allowed : 3.00 Hours ] [ Maximum Marks : 70
AÔÄøµPÒ : (1) AøÚzx ÂÚõUPЮ \›¯õP £vÁõQ EÒÍuõ GߣuøÚ
\›£õºzxU öPõÒÍÄ®. Aa_¨£vÂÀ SøÓ°¸¨¤ß AøÓU
PsPõo¨£õÍ›h® EhÚi¯õPz öu›ÂUPÄ®.
(2) }»® AÀ»x P¸¨¦ ø©°øÚ ©mk÷© GÊxÁuØS®,
AiU÷PõikÁuØS® £¯ß£kzu ÷Ásk®. £h[PÒ ÁøµÁuØS
ö£ß]À £¯ß£kzuÄ®.
(3) ÷uøÁ¯õÚ Ch[PÎÀ £h[PÒ ÁøµP.
Instructions : (1) Check the question paper for fairness of printing. If there is any lack of
fairness, inform the Hall Supervisor immediately.
(2) Use Blue or Black ink to write and underline and pencil to draw diagrams.
(3) Draw diagrams wherever necessary.
£Sv & I / PART - I
SÔ¨¦ : (i) AøÚzx ÂÚõUPÐUS® Âøh¯ÎUPÄ®. 15x1=15
(ii) öPõkUP¨£mkÒÍ ©õØÖ ÂøhPÎÀ ªPÄ® Hئøh¯
Âøhø¯z ÷uº¢öukzxU SÔ±mkhß Âøh°øÚ²® ÷\ºzx
GÊuÄ®.
Note : (i) Answer all the questions.
(ii) Choose the most appropriate answer from the given four alternatives and
write the option code and the corresponding answer.
[ v¸¨¦P / Turn over
Page 3
3462 (NS) 2
1. Aö»Uéõshº ¤Í®ª[ Áͺzu ‰» ÁͺuÍzvÀ ö£Û]¼ß ö\¯ø»U
Pshõº. ö£Û]¼ß ‰»® Áͺa] uøh¨£mh £õUj›¯õ Gx ?
(A) ø©U÷Põ¤»õì©õ
(B) £õUj›÷¯õ£õä
(C) ö£Û]¼¯® ö|õm÷hmh®
(D) ìøhø£÷»õPõUPì B›¯ì
Alexander Flemming observed the action of penicillin on the culture plate. Which
bacteria was inhibited by Penicillin ?
(a) Mycoplasma
(b) Bacteriophage
(c) Penicillium notatum
(d) Staphylococcus aureus
2. Gί ~s÷nõUQø¯ •uÀ •øÓ¯õP £¯ß£kzv¯Áº :
(A) ¿°ì £õ´a\º (B) Bshß Áõß ¿¯ÁßíõU
(C) Cµõ£ºm íúU (D) \UP›¯õ
The first person to use a simple microscope :
(a) Louis Pasteur (b) Anton Von Leewenhoek
(c) Robert Hooke (d) Zaccharia
3. Gߣx, Qµõ® \õ¯÷©ØÖ® •øÓ°À •ußø© \õ¯©õP
________
£¯ß£kQÓx.
(A) \õ¨µõÛß (B) A÷¯õiß
(C) Q›ìhÀ Á¯ö»m (D) »õU÷hõ¥ÚõÀ Põmhß ¦Ñ
_________ is used as a primary stain in gram staining.
(a) Saffranin (b) Iodine
(c) Crystal Violet (d) Lactophenol cotton blue
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3 3462 (NS)
4. \›¯õÚ Cøn°øÚ ÷uº¢öukUPÄ®.
(A) ißhø»÷\\ß & Dµ öÁ¨£®, }µõÂ, 908C
(B) ªßöÁ¨£ \©{ø» Ak¨¦ & Dµ öÁ¨£®, 121 £Äsm, 158C
(C) öÁ¨£ PõØÖ Ak¨¦ & Dµ öÁ¨£®, 1608C, 1 ©o ÷|µ®
(D) £õì\øµ÷\åß & Dµ öÁ¨£®, 728C, 1 ©o ÷|µ®
Identify the correct pair :
(a) Tyndallization - Moist heat, steaming at 908C
(b) Autoclave - Moist heat, 121 lbs 158C
(c) Hot air oven - Moist heat, 1608C for one hour
(d) Pasteurization - Moist heat, 728C for one hour
5. `÷hõ÷©õÚì H÷µõâ÷Úõ\õ {Óª°ß {Ó® GßÚ ?
(A) £aø\ (B) ]Á¨¦
(C) u[P{Ó ©g\Ò (D) ©g\Ò
The pigment colour of Pseudomonas aeruginosa is :
(a) Green (b) Red
(c) Golden yellow (d) Yellow
6. öPmk¨÷£õÚ FÖPõ°¼¸¢x ¤›zöukUP¨£k® £õUj›¯õ GxÁõP
C¸UP»õ® ?
(A) SøÓ¢u öÁ¨£{ø» uõ[QPÒ
(B) AvP öÁ¨£{ø» ¸®¤PÒ
(C) EÁº ¸®¤PÒ
(D) £õ÷µõ¤Àì
A bacteria isolated from spoilt pickle is likely to be a :
(a) Psychrophile
(b) Thermophile
(c) Halophile
(d) Barophile
[ v¸¨¦P / Turn over
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3462 (NS) 4
7. ø»¨÷£õ£õ¼\õUPøµm G¢u ö\À _Á›À EÒÍx ?
(A) Qµõ® £õ]iÆ £õUj›¯õ (B) Qµõ® ö|PiÆ £õUj›¯õ
(C) Gèö\›]¯õ ÷Põø» (D) (B) ©ØÖ® (C)
Lipopolysaccharide is found in cell wall of :
(a) Gram positive bacteria (b) Gram negative bacteria
(c) Escherichia coli (d) Both (b) and (c)
8. §ª°ß A¸Q¾ÒÍ ÁΩsh» AkUS Gx ?
(A) öuº÷©õ줯º (B) ìmµõm÷hõ줯º
(C) «÷\õ줯º (D) m÷µõ÷£õ줯º
Which of the following is the layer of Atmosphere closer to the earth ?
(a) Thermosphere (b) Stratosphere
(c) Mesosphere (d) Troposphere
9. ¼a\ß GßÓ Tmk°› ÁõÌUøP°À, £õ] Gߣx :
(A) §gø\ (B) ©UPaö\´ÁÚ
(C) •uÀ{ø» EØ£zv¯õͺ (D) ~Pº÷Áõº
The phycosymbiont in a lichen is a :
(a) Fungus (b) Decomposer
(c) Primary producer (d) Consumer
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5 3462 (NS)
10. TØÖ (A) : ö©zu÷Úõöáß Põº£ß&øh&BUøéøh «z÷uÚõP ©õØÖQÓx.
TØÖ (B) : ö©zu÷ÚõöáÛ]ì Gߣx PõØÔÀ»õ©À |øhö£Ö® {PÌÄ.
(A) TØÖ (B)&¯õÚx TØÖ (A)&ÂØS ö£õ¸zu©õÚx AÀ»
(B) TØÖ (A) ©mk÷© \›¯õÚx
(C) TØÖ (B) uÁÓõÚx
(D) TØÖ (A)&¯õÚx TØÖ (B)&¯õÀ Bu›UP¨£kQÓx
Statement (A) : Methanogens convert carbon dioxide to methane.
Statement (B) : Methanogenesis is an anaerobic process.
(a) Statement (A) is not related to Statement (B).
(b) Statement (A) alone is true.
(c) Statement (B) is false.
(d) Statement (A) is supported by Statement (B).
11. ³÷P›÷¯õmiU J¸ö\À E°›PÒ GÆÁøP E»Pzøua \õº¢ux :
(A) §gø\PÒ (B) ö©õÛµõ
(C) ¦÷µõiìhõ (D) uõÁµ[PÒ
Unicellular Eukaryotes belong to which of the following kingdom ?
(a) Fungi (b) Monera
(c) Protista (d) Plantae
12. ]¤¼ì J¸ ________ BS®.
(A) ]Ö}µP ©sh» ÷|õ´
(B) £õÀÂøÚ ÷|õ´
(C) _Áõ\ ©sh» ÷|õ´
(D) Cøµ¨ø£ ShÀ ÷|õ´
Syphilis is a ________.
(a) Urinary tract disease
(b) Sexually transmitted disease
(c) Respiratory tract disease
(d) Gastro tract disease
[ v¸¨¦P / Turn over
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3462 (NS) 6
13. ‰øÍ uskÁh vµÁ® (CSF) ¤ßÁ¸ÁÚÁØÔÀ G[S Põn¨£k® ?
(A) xøn i³µõ CøhöÁÎ
(B) ö£›ÁõìS»õº CøhöÁÎ
(C) xøn AµUÚõ´k CøhöÁÎ
(D) ©søh KmiØS® i³µõ ÷©mh¸US® Cøh°À
Cerebrospinal Fluid (CSF) is present in which of the following ?
(a) Sub-dural space
(b) Perivascular space
(c) Sub-arachnoid space
(d) Between skull and dura mater
14. Bsi£õiPÒ GvºÂøÚ ö\´²® BßiöáÛU uÍ[PÒ ________ GÚ
AøÇUP¨£kQßÓÚ.
(A) G¤÷hõ¨PÒ (B) uk¨£õØÓÀ
(C) ÷P›¯ºPÒ (D) £õµõ÷hõ¨
Antigenic site with which antibodies react are called :
(a) Epitopes (b) Immunogens
(c) Carriers (d) Paratope
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7 3462 (NS)
15. RÌUPsh TØÖPÎÀ Gx \› ?
(i) Áõm\ß ©ØÖ® Q›U ©õv› i.Gß.H. GÝ® Aø©¨¦ B BS®.
(ii) i.Gß.H.Âß B &Aø©¨¦ Chx £UP® öPõshx
(iii) i.Gß.H.Âß A &Aø©¨¦ Á»x £UP® öPõshx
(iv) i.Gß.H. A &Aø©¨¤À J¸ _ǼÀ HØ£k® Põµ Cøn¨¦PÒ 10
(A) AøÚzx TØÖPЮ uÁÖ
(B) AøÚzx TØÖPЮ \›
(C) (i) ©ØÖ® (iii) ©mk® \›
(D) (i) ©ØÖ® (ii) ©mk® \›
Which of the following statements are true ?
(i) Watson and Crick model of DNA is B-form
(ii) B-form DNA is left handed form
(iii) A-form DNA is right handed form
(iv) A-form DNA has 10 base pairs per helical turn
(a) All the statements are false
(b) All the statements are true
(c) Only (i) and (iii) are true
(d) Only (i) and (ii) are true
£Sv & II / PART - II
SÔ¨¦ : GøÁ÷¯Ý® BÖ ÂÚõUPÐUS Âøh¯ÎUPÄ®. ÂÚõ Gs 24 &US
Pmhõ¯©õP Âøh¯ÎUPÄ®. 6x2=12
Note : Answer any six questions. Question No. 24 is compulsory.
16. HEPA & ›ÁõUPzøu GÊxP. HEPA & ÁiPmi°ß £¯ß ¯õx ?
Expand HEPA. What is the use of HEPA filters ?
17. ö©UPõßQ APõº Hß ¤›zuÔ FhP® GßÓøÇUP¨£kQÓx ?
Why is MacConkey’s agar called a differential medium ?
[ v¸¨¦P / Turn over
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3462 (NS) 8
18. ©s BUi÷Úõø©^mêß £oPøÍ GÊv, Cµsk Euõµn® u¸P.
Describe the functions of Actinomycetes and give two examples of Actinomycetes in
soil.
19. ¤¼÷¯õ©õº¤U £õUj›¯õ GßÓõÀ GßÚ ? GkzxUPõmk u¸P.
What are pleomorphic bacteria ? Give an example.
20. (A) øµ÷\õ¤¯® Áͺ¨¦ FhPzvß ö£¯º GßÚ ?
(B) §gø\ §a]U öPõÀ¼PÒ Cµsiß ö£¯ºPøÍ GÊxP.
(a) Name the media used to grow Rhizobium.
(b) Name two Mycopesticides.
21. E°º Eµ[PÒ & Áøµ¯ÖUPÄ®. AÁØÔß £¯ßPÒ GøÁ÷¯Ý® CµsiøÚ
GÊxP.
Define Biofertilizers. Write any two of its advantages.
22. ¤ßÁ¸ÁÚÁØÔß ÷|õ´zöuõØÔøÚ PshÔP.
ö\g\¸©® AUQ, ]Ö}µP ø£ ÷|õ´zöuõØÖ, ö\ AÇØ], æöPÀ»õêì
Identify the type of infections for the following :
Erysipela, Cystitis, Otitis media, Shigellosis
23. ©Ûu Cøµ¨ø£ShÀ ©sh»zvÀ Põn¨£k® C¯À¦{ø» £ØÔ GÊxP ?
Give the normal flora of the gastrointestinal tract of humans.
24. Qµõ®&£õ]iÆ £õUj›¯õÂß |õßS GkzxUPõmkPÒ u¸P.
Give examples of any four gram positive bacteria.
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9 3462 (NS)
£Sv & III / PART - III
SÔ¨¦ : GøÁ÷¯Ý® BÖ ÂÚõUPÐUS Âøh¯ÎUPÄ®. ÂÚõ Gs 33&US
Pmhõ¯©õP Âøh¯ÎUPÄ®. 6x3=18
Note : Answer any six questions. Question No. 33 is compulsory.
25. EÒÍõº¢u ÷|õ´zuk¨£õØÓÀ ©ØÖ® ö£Ó¨£mh ÷|õ´zuk¨£õØÓÀ
÷ÁÖ£kzxP.
Distinguish between Innate Immunity and Acquired Immunity.
26. (A) ¤øµm ¥Àk ~s÷nõUQ°À G¢u £õP® JÎUT®¤øÚ |ÊÁzvß
÷©À S¯a ö\´QÓx?
(B) J¸ ö£õ¸Í¸S ÂÀø»°ß JÎ ÷\P›US® vÓß Gx ?
(C) JßÖ ÷\º¢xÒÍ ]Ô¯ ö£õ¸ÒPøÍ ÷ÁÖ¨£kzvU Põmk®
ÂÀø»°ß vÓß ö£¯º GßÚ ?
Name the following :
(a) A part of the bright field microscope that focuses a cone of light on the slide.
(b) The value representing the light gathering capacity of an objective lens.
(c) The capacity of a lens to separate or distinguish between small objects that are
close together.
27. ÁøP¨£õmi¯¼ß ÷|õUP[PÒ ¯õøÁ ?
What are the objectives of taxonomy ?
28. \õn G›Áõ² Eø»°ß £h® Áøµ¢x £õP[PøÍ SÔUPÄ®.
Draw and label biogas digester.
29. HuõÁx J¸ Bsh÷PõÚìiU öuõhºø£ GkzxUPõmkhß ÂÁ›UPÄ®.
Explain any one Antagonistic interaction with an example.
30. iø|m›L¤÷Påß £ØÔ ÂÁ›UPÄ®.
Explain denitrification.
[ v¸¨¦P / Turn over
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3462 (NS) 10
31. RÌPshÁØÖÒ } AÔÁx GßÚ ?
(i) Áµ®¦USm£mh öuõØÖ
(ii) ÷|õ´ Phzv
(iii) £õUjŸª¯õ
What do you understand by the following ?
(i) localized infections
(ii) healthy carrier
(iii) Bacteremia
32. ¤ß u[Q¯ CøÇ GßÓõÀ GßÚ ? Ax GÆÁõÖ E¸ÁõQÓx ?
What is lagging strand ? How is it synthesized ?
33. uß Â¸®¤ PõØÓØÓ _Áõ]PÒ & Áøµ¯ÖUPÄ®. HuõÁx J¸ GkzxUPõmkz
u¸P.
Define Facultative anaerobes. Give an example.
£Sv & IV / PART - IV
SÔ¨¦ : AøÚzx ÂÚõUPÐUS® Âøh¯ÎUPÄ®. 5x5=25
Note : Answer all the questions.
34. (A) uõ÷Ú ÷uõßÔ¯ uø»•øÓ ÷Põm£õmiøÚ ¿° £õ´a\º GÆÁõÖ
©Özuõº ?
AÀ»x
(B) hõºU ¥Àk ~s÷nõUQø¯¨ £ØÔ ›ÁõP ÂÁ›UPÄ®.
(a) How was spontaneous generation theory disproved by Louis Pasteur ?
OR
(b) Discuss Dark Field Microscope.
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11 3462 (NS)
35. (A) ö£õxÁõP¨ £¯ß£kzu¨£k® \õ¯[PÒ GøÁ÷¯Ý® I¢vøÚ¨ £ØÔ
GÊv Auß £¯ßPøÍ £mi¯¼kP.
AÀ»x
(B) APõº Gߣx ¯õx ? Ax Gv¼¸¢x ¤›UP¨£kQÓx ? APõ›ß
£¯ßPÒ ¯õøÁ ?
(a) List any five commonly used stains and their applications.
OR
(b) What is agar ? Where is it extracted from ? What are its uses ?
36. (A) C¯Ø¤¯À •øÓ°À ~sq°º }UP® £ØÔ¯ ÁÈ•øÓ Áøµ£h®
ÁøµP.
AÀ»x
(B) P\k ]øuÄ {PÌøÁ¨ £ØÔ J¸ SÔ¨¦ ÁøµP.
(a) Represent Physical methods of sterilization by a Flow Chart.
OR
(b) Write a note on activated sludge process.
37. (A) ÁõÌÁÚ PnURk •øÓPøÍ ÂÁ›UPÄ®.
AÀ»x
(B) £õUj›¯õ vßSȯ[PÍõÀ ÂÊ[P¨£k® ÷£õx |øhö£Ö® £»
{ø»PÒ £ØÔ GÊxP.
(a) Explain the methods used to determine the viable count.
OR
(b) Write the steps involved in ingestion of bacteria by Phagocytic cells.
[ v¸¨¦P / Turn over
Page 13
3462 (NS) 12
38. (A) £õUj›¯õ |P¸u¾US ÷uøÁ¨£k® xøn²Ö¨¦ Aø©¨ø£ £ØÔ
›ÁõP ÂÁ›UPÄ®.
AÀ»x
(B) i.Gß.H. Cµmizu¼ß {PÌÂÀ xÁUP{ø»°ß •UQ¯©õÚ £iPøÍ
GÊxP.
(a) Describe the structure of bacterial appendage involved in motility.
OR
(b) Write the major steps involved in Initiation of DNA Replication.
-o0o-