DUET 2021 Question Paper M.Phil Ph.D in Botany

DU MPhil PhD in Botany
Topic:‐ BOT MPHIL

1) Cryphonectria hypovirus 1 overcomes RNA silencing mediated defence response of its fungal host Cryphonectria
parasitica by

[Question ID = 10038]
1. Production of a host encoded suppressor protein

[Option ID = 40149]
2. Production of a virus encoded suppressor protein

[Option ID = 40150]
3. Down‐regulation of host Dicer‐like 2 (DCL2) nuclease

[Option ID = 40151]
4. Inducing a feedback loop that activates SAR in the host plant of C. parasitica

[Option ID = 40152]

2) Genomic flexibility of prokaryotic viruses cannot be attributed to

[Question ID = 10039]
1. Frequent recombination

[Option ID = 40153]
2. Lateral gene transfer between viruses and their host

[Option ID = 40154]
3. Host modification system

[Option ID = 40155]
4. Gene gain and loss

[Option ID = 40156]

3) In Lysogenic phase of the life cycle of bacteriophages, ‘Lysogen’ is the[Question ID = 10040]
1. Bacterial host cell that contains viral prophage [Option ID = 40157]
2. Viral genetic material that enters bacterial host cells [Option ID = 40158]
3. Viral protein that helps in entry of prophage into bacterial host cells [Option ID = 40159]
4. Bacterial protein that helps in entry of prophage into bacterial host cells [Option ID = 40160]

4) Which of the following differentiates Archaebacteria from Eubacteria?[Question ID = 10041]
1. In archaebacteria, membrane lipids have branched hydrocarbons linked to glycerol by ester bonds [Option ID = 40161]
2. In archaebacteria, membrane lipids have branched hydrocarbons linked to glycerol by ether bonds [Option ID = 40162]
3. In eubacteria, cells walls have peptidoglycans N‐acetylglucosamine and N‐acetyltalosaminuronic acid [Option ID = 40163]
4. In eubacteria, membrane lipids have unbranched hydrocarbons linked to glycerol by ether bonds [Option ID = 40164]

5) Which of the following is a false statement?[Question ID = 10042]
1. In phylograms, branch lengths are proportional to the amount of change [Option ID = 40165]
2. In phylogenetic tress, free rotation is possible around each of the horizontal branches at their nodes [Option ID = 40166]
3. Mitochondrial genes accumulate mutations more rapidly as compared to chloroplast genes in plants [Option ID = 40167]
4. Super tree is a single phylogenetic tree that compiles many trees with overlapping taxa into a single comprehensive tree [Option ID = 40168]

6) Which of the following statements related to nomenclature is true?

[Question ID = 10043]
1. Strychnos nux‐vomica is a trinomial

[Option ID = 40169]
2. Bambusa bambos is a tautonym

[Option ID = 40170]
3. Variety is designated by the abbreviation ‘var.’ in italics

[Option ID = 40171]
4. The abbreviation cv. should not be used to denote a cultivar

[Option ID = 40172]

7) In a situation where the calculated t value is more than the critical t value, which one of the following options is
correct?[Question ID = 10044]
1. Accept null hypothesis [Option ID = 40173]
2. Reject null hypothesis [Option ID = 40174]
3. Remove pseudo‐replication of data [Option ID = 40175]
4. Reject alternate hypothesis [Option ID = 40176]

8) PhyloCode and Rank‐based systems are similar in all of the following, except[Question ID = 10045]
1. Establishment of a name requires publication and registration. [Option ID = 40177]
2. Conservation rule is used to give priority to a later published name. [Option ID = 40178]
3. Priority is taken into consideration while determining the correct name. [Option ID = 40179]
4. Date of publication is the main criteria for establishing precedence. [Option ID = 40180]

9) Which of the following is not an example of homoplasy?

[Question ID = 10046]
1. Leaves of pitcher plant and spines of cactus

[Option ID = 40181]
2. Tendrils in angiosperms

[Option ID = 40182]
3. Gametophytic leaves of liverwort and Sporophytic leaves of lycopod

[Option ID = 40183]
4. Absence of perianth in Lemna

[Option ID = 40184]

10) Which of the following is a characteristic feature of ancestral angiosperm flowers?[Question ID = 10047]
1. Unisexual flowers [Option ID = 40185]
2. Undifferentiated perianth [Option ID = 40186]
3. Zygomorphic flowers [Option ID = 40187]
4. Spirally arrangement stamens [Option ID = 40188]

11) Angiosperms are estimated to have evolved about[Question ID = 10048]
1. 267 – 187 Mya [Option ID = 40189]
2. 193 – 146 Mya [Option ID = 40190]
3. 420 ​– 417 Mya [Option ID = 40191]
4. 184 – 131 Mya [Option ID = 40192]

12) Which of the following is incorrectly associated?[Question ID = 10049]
1. UPGMA – Cluster analysis [Option ID = 40193]
2. Bayesian Analysis – Posterior probabilities [Option ID = 40194]
3. Maximum Likelihood ‐ Occam's razor [Option ID = 40195]
4. Neighbor Joining ‐ Phylogram [Option ID = 40196]

13) Importance of axial polarity in plant morphogenesis has been demonstrated by inducing symmetric division in the
zygote of which mutant of Arabidopsis thaliana?

[Question ID = 10050]
1. gnom

[Option ID = 40197]
2. fis

[Option ID = 40198]
3. stm

[Option ID = 40199]
4. wus

[Option ID = 40200]

14) In organ developmental studies, clonal analysis refers to[Question ID = 10051]
1. determining the number of clones formed by bulbils. [Option ID = 40201]
2. identifying in vitro induced somaclonal variation. [Option ID = 40202]
3. mapping of cell lineages derived from an apical cell. [Option ID = 40203]
4. identification of apomictic seeds. [Option ID = 40204]

15) Which one of the following is a commercial source of phloem fibers?

[Question ID = 10052]
1. Corchorus capsularis

[Option ID = 40205]
2. Agave sisalana

[Option ID = 40206]
3. Musa textilis

[Option ID = 40207]
4. Gossypium hirsutum

[Option ID = 40208]

16) Which of the following cells are implicated in perception of gravity in a root?[Question ID = 10053]
1. Stem cells [Option ID = 40209]

2. Root apical meristem cells [Option ID = 40210]
3. Statocyte cells [Option ID = 40211]
4. Border cells [Option ID = 40212]

17) Following are some of the mechanisms of humoral and cell‐mediated immune responses to viruses. Match the effector
molecule/cells (Column A) with their activities (Column B).

Column A Column B
A. Natural Killer (NK) Cells I. Prevents infection or re‐infection
B. Cytotoxic T‐Cells (CTLs) II. Lysis of enveloped viral particles
C. IFN‐g III. Kills virus‐infected self‐cells
D. IgA IV. Direct anti‐viral activity
E. Antibody‐activated complements V. Kill virus‐infected cells by ADCC

[Question ID = 10054]
1. A ‐ V, B ‐ III, C ‐ IV, D ‐ I, E ‐ II [Option ID = 40213]
2. A ‐ III, B ‐ V, C ‐ IV, D ‐ I, E ‐ II [Option ID = 40214]
3. A ‐ IV, B ‐ V, C ‐ III, D ‐ II, E ‐ I [Option ID = 40215]
4. A ‐ V, B ‐ IV, C ‐ II, D ‐ III, E ‐ I [Option ID = 40216]

18) Positioning of a flower on an inflorescence requires interplay of auxin with which one of the following genes?

[Question ID = 10055]
1. APETALA1

[Option ID = 40217]
2. AGAMOUS

[Option ID = 40218]
3. FT

[Option ID = 40219]
4. LEAFY

[Option ID = 40220]

19) Which of the following pair of genes represents the correct combination of orthologs involved in floral organ identity
among Arabidopsis thaliana and Antirrhinum majus?

[Question ID = 10056]
1. APETALA3 ‐ GLOBOSA

[Option ID = 40221]
2. APETALA3 ‐ DEFICIENS

[Option ID = 40222]
3. APETALA3 ‐ PLENA

[Option ID = 40223]
4. APETALA3 ‐ SUPERWOMAN1

[Option ID = 40224]

20) Which of the following is incorrect for E.coli protein over‐expression/purification using pBAD expression system?

[Question ID = 10057]
1. It is an L‐arabinose dependent process

[Option ID = 40225]
2. It is designed for the tight control of background expression

[Option ID = 40226]
3. Linear increase in gene expression with increasing inducer concentration is observed

[Option ID = 40227]
4. Over‐expressed protein is purified using immobilized metal affinity chromatography (IMAC) with Ni 2+

[Option ID = 40228]

21) Which of the following statements is not true for commercial Bt cotton plants expressing a codon optimized transgene?

[Question ID = 10058]
1. Expression levels of the transgenic Bt endotoxin is high

[Option ID = 40229]
2. LD50 provided by transgenic Bt endotoxin is adequate for control of targeted insect pest.

[Option ID = 40230]
3. Build‐up of resistance against the targeted insect pest is less likely

[Option ID = 40231]
4. Chemical synthesis of the transgene is usually necessary

[Option ID = 40232]

22) Which of the following cannot be considered an example of the co‐evolutionary arms race between Plant and its
Herbivore?

[Question ID = 10059]
1. Production of Glucosinolates in Brassicales against cabbage butterflies

[Option ID = 40233]
2. Difference in the number and density of trichomes on leaf surfaces.

[Option ID = 40234]
3. Release of Indole by plant leaves upon attack.

[Option ID = 40235]
4. Release of cysteine proteases in latex of Euphorbia species upon wounding

[Option ID = 40236]

23) Which of the following molecules is most likely to be associated with induced, indirect, responses of plants to
herbivory?[Question ID = 10060]
1. Defensin [Option ID = 40237]
2. Nonatriene [Option ID = 40238]
3. Hydrogen peroxide [Option ID = 40239]
4. Kunitz trypsin inhibitor [Option ID = 40240]

24) Tobacco plants were transformed with an antisense construct that silenced the gene encoding lipoxygenase (as lox‐).
Which of the following is not likely to be an outcome of growing these mutants in the field?

[Question ID = 10061]
1. Impaired production of Jasmonic acid

[Option ID = 40241]
2. Reduced fitness costs due to lowered production of PR proteins

[Option ID = 40242]
3. Increased resistance against attack by chewing insects

[Option ID = 40243]
4. Change in type and groups of insects that typically attack tobacco plants

[Option ID = 40244]

25) A group of scientists wanted to create transgenic plants that were resistant to a potyvirus (+ stranded RNA genome)
using VIGS. Which of the following transgenes would they prefer?[Question ID = 10062]
1. A highly conserved, non‐coding fragment of the coat protein gene [Option ID = 40245]
2. A highly conserved, full‐length, translatable coat protein gene [Option ID = 40246]
3. A variable region of a VIGS suppressor gene, so that multiple viruses can be targeted [Option ID = 40247]
4. A variable region of a VIGS suppressor gene, so that multiple viral strains can be targeted [Option ID = 40248]

26) Field resistance in the pink cotton bollworm to 1st generation transgenic Bt cotton in India can be best attributed to:
[Question ID = 10063]
1. Single mutation event in the E‐Cadherin gene [Option ID = 40249]
2. Alternative splicing events in the E‐Cadherin gene [Option ID = 40250]
3. Impaired binding of Cry I Ac domain II to aminopeptidase receptor in the gut [Option ID = 40251]
4. Lack of adequate refugia for insects susceptible to Cry I Ac [Option ID = 40252]

27) The pheromone synthesized via β‐carotene, and responsible for sexual differentiation in Zygomycetes is[Question ID =
10064]
1. Trisporic acid [Option ID = 40253]
2. Glomalin [Option ID = 40254]
3. Acetic acid [Option ID = 40255]
4. Carotenoids [Option ID = 40256]

28) Plant signals that are released by roots to induce hyphal branching in arbuscular mycorrhizal fungi is:[Question ID =
10065]
1. Strigalactones [Option ID = 40257]
2. Flavanoids [Option ID = 40258]
3. Lectins [Option ID = 40259]
4. Glycoproteins [Option ID = 40260]

29) Penetration of host by Magnaporthe oryzae is facilitated by[Question ID = 10066]
1. Turgor pressure generated in the appressorium [Option ID = 40261]
2. Cell wall hydrolyzing enzymes [Option ID = 40262]
3. Combination of turgor pressure generated in the appressorium and hydrolyzing enzymes [Option ID = 40263]
4. Insects, natural opening and wounds [Option ID = 40264]

30) What is the rate of population growth per unit of time when r = 0.1, N = 100 and K = 300?[Question ID = 10067]

1. 4.66 [Option ID = 40265]
2. 6.66 [Option ID = 40266]
3. 8.66 [Option ID = 40267]
4. 10.66 [Option ID = 40268]

31) Robert Paine (1966) proposed that the feeding habit of a few species has a direct influence on the community
structure. He called these species as[Question ID = 10068]
1. Umbrella species [Option ID = 40269]
2. Priority species [Option ID = 40270]
3. Keystone species [Option ID = 40271]
4. Flagship species [Option ID = 40272]

32) In a static life table, the notation lx = nx1 / nxo is denoted by

[Question ID = 10069]
1. Number of individuals dying at the beginning of “age interval x”, and the beginning of “age interval x+1”

[Option ID = 40273]
2. Number of individuals alive during “age interval x”

[Option ID = 40274]
3. Number of individuals at the beginning of “age interval x+1”

[Option ID = 40275]
4. Proportion of original individuals (%) surviving to beginning of “age interval x”

[Option ID = 40276]

33) Armen Takhtajan (1986) divided the floristic regions of the world into six major kingdoms. The Indian region comes
under ………….. kingdoms.[Question ID = 10070]
1. Palaeotropical and Neotropical [Option ID = 40277]
2. Holarctic and Neotropical [Option ID = 40278]
3. Holarctic and Palaeotropical [Option ID = 40279]
4. Holarctic and Cape [Option ID = 40280]

34) The equilibrium model of Island biogeography is a balance between which one of the following combination of
phenomena?[Question ID = 10071]
1. Immigration and extinction [Option ID = 40281]
2. Immigration and emigration [Option ID = 40282]
3. Extinction and emigration [Option ID = 40283]
4. Extinction and species isolation [Option ID = 40284]

35) Which one of the following factors could potentially explain variations in expression levels of a transgene among
different transgenic plants generated using the same T‐DNA construct?[Question ID = 10072]
1. Strength of promoter used to express the transgene [Option ID = 40285]
2. Stability of the mRNA encoded by the transgene [Option ID = 40286]
3. Codon usage of the transgene [Option ID = 40287]
4. Site of integration of the transgene in the nuclear genome [Option ID = 40288]

36) Which one of the following experimental approaches cannot be used for evaluating transgene expression levels in
transgenic plants?

[Question ID = 10073]
1. Northern blotting

[Option ID = 40289]
2. ELISA

[Option ID = 40290]
3. Western blotting

[Option ID = 40291]
4. Southern blotting

[Option ID = 40292]

37) Match List I with List II
List I List II
A. Papaver somniferum I. Model Legume
B. Lotus japonicus II. Oilseed crop
C. Populus trichocarpa III. Morphine
D. Carthamus tinctorius IV. First tree genome sequence
Choose the correct answer from the options given below:

[Question ID = 10074]
1. A ‐ II, B ‐ IV, C ‐ I, D ‐ III [Option ID = 40293]
2. A ‐ II, B ‐ III, C ‐ I, D ‐ IV [Option ID = 40294]
3. A ‐ IV, B ‐ III, C ‐ II, D ‐ I [Option ID = 40295]

4. A ‐ III, B ‐ I, C ‐ IV, D ‐ II [Option ID = 40296]

38) Which one of the following pairs is matched correctly?

[Question ID = 10075]
1. Crop domestication – Increased genetic diversity

[Option ID = 40297]
2. High cytokinin : auxin ratio – Root morphogenesis

[Option ID = 40298]
3. Integration of T‐DNA in nuclear genome – Homologous recombination

[Option ID = 40299]
4. Non‐conditional negative selection marker gene – Developmentally regulated promoter

[Option ID = 40300]

39) Crosses between maize and teosinte results in[Question ID = 10076]
1. No hybrids [Option ID = 40301]
2. Fertile hybrids [Option ID = 40302]
3. Sterile hybrids [Option ID = 40303]
4. Eliminate one genome resulting in haploid production [Option ID = 40304]

40) Which of the following statements is false for lactose operon mutants having mutation in operator region?

[Question ID = 10077]
1. Repressor molecule will not be able to bind to the operator.

[Option ID = 40305]
2. The mutant will have constitutive expression of the lac operon in absence of glucose.

[Option ID = 40306]
3. The mutant will have constitutive expression of the lac operon in presence of cAMP.

[Option ID = 40307]
4. The mutant will have inducible expression in the presence of glucose.

[Option ID = 40308]

41) Which of the following is false for quantitative traits?

[Question ID = 10078]
1. Several genotypes may produce same phenotype

[Option ID = 40309]
2. Individual genes governing the trait do not follow Mendelian inheritance

[Option ID = 40310]
3. They are polygenic in nature

[Option ID = 40311]
4. The phenotypic segregation ratios are not similar to the typical Mendelian ratios

[Option ID = 40312]

42) Which of the following is a hybridization‐based DNA marker system?[Question ID = 10079]
1. RFLP [Option ID = 40313]
2. SSR [Option ID = 40314]
3. RAPD [Option ID = 40315]
4. AFLP [Option ID = 40316]

43) Restarting transcription by generating a new 3’‐end by the stalled RNA polymerase requires:

[Question ID = 10080]
1. TFIIS in eukaryotes, and Gre factors in prokaryotes

[Option ID = 40317]
2. TFIID in both eukaryotes and prokaryotes

[Option ID = 40318]
3. TFIIE in eukaryotes, and rut factors in prokaryotes

[Option ID = 40319]
4. TFIIH in eukaryotes, and rho in prokaryotes

[Option ID = 40320]

44) A deletion in the thumb domain of DNA polymerase will affect

[Question ID = 10081]
1. binding of DNA as it exits the active site thus affecting only processivity

[Option ID = 40321]
2. both rotation and positioning of dsDNA template at the active site

[Option ID = 40322]
3. catalytic site formation, polymerization and proofreading function

[Option ID = 40323]
4. rotation of dsDNA template only

[Option ID = 40324]

45) Which of the following processes do not play a role in polymerization of G‐actin into F‐actin?

[Question ID = 10082]
1. Disassociation of G‐actin bound to Profilin

[Option ID = 40325]
2. Dynamic instability of the actin filaments

[Option ID = 40326]
3. Increase in levels of intracellular G‐actin monomers

[Option ID = 40327]
4. Polarity of phosphorylation and dephosphorylation along the actin filament

[Option ID = 40328]

46) Entry of a newly synthesized Type I membrane protein into the endoplasmic reticulum is facilitated by[Question ID =
10083]
1. N terminal cleavable signal peptide [Option ID = 40329]
2. C terminal H/KDEL cleavable sequence motif [Option ID = 40330]
3. C terminal di lysine cleavable sequence motif [Option ID = 40331]
4. Binding of ERD2 like receptor [Option ID = 40332]

47) Which of the following represents a correct combination?

[Question ID = 10084]
1. Maryland mammoth variety– Chailakhyan‐hormonal theory of plant development

[Option ID = 40333]
2. Carrot protoplast– Waddington‐Totipotency

[Option ID = 40334]
3. H3K9methylation– piwiRNA‐euchromatin

[Option ID = 40335]
4. Maryland mammoth variety‐Garner and Allard‐Photoperiod

[Option ID = 40336]

48) Which of the following is not a type of β‐lactam antimicrobial?

[Question ID = 10085]
1. Penicillin

[Option ID = 40337]
2. Glycopeptides

[Option ID = 40338]
3. Cephalosporins

[Option ID = 40339]
4. Monobactams

[Option ID = 40340]

49) Which of the following is not true for Archaea?

[Question ID = 10086]
1. Cell wall is made up of pseudopeptidoglycan

[Option ID = 40341]
2. They have lipid membrane of ester bonds with fatty acids

[Option ID = 40342]
3. Archaea are Methanogens, Halophiles, Thermoacidophiles

[Option ID = 40343]
4. Archaea reproduce asexually by binary fission, fragmentation, or by the budding process.

[Option ID = 40344]

50) Which one of the following would be used to analyze homology between a translated nucleotide sequence (as query)
and a protein database (subject)?[Question ID = 10087]
1. BLASTn [Option ID = 40345]
2. BLASTx [Option ID = 40346]
3. tBLASTn [Option ID = 40347]
4. BLASTp [Option ID = 40348]