AHSEC HS 2nd Year Question Paper 2022 Biotechnology

Total number of pages - 8
32T BTEC

20.22

BIOTECHNOLOGY
(Theory)

Full Marks : 70
Pass Marks : 21

Time : Three hours

The fixures in the margin inilicate full marks
for the questions.

Q. No. I cnrrics 7 nnrk each 1x8=8

2x10=2O
Q. No. 2-77 catry 2 marks each

Q. No. 72-20 carry 3 marks each 3x9=27

Q. No. 27-23 carry 5 ma*s each 5x3=15

Total = 70

Contd.

1. (a) What does PCR stand for ?

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(b) Define stem cell. 7

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@ What are DNA ligases ? 1

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(d) What do you mean by'vectol ?

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k) What is 'Rh' factor ?

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(fl What is bioremediation ?

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@) What is Palindromic sequence ? 1

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(h) Define the term 'Totipotency'. 1

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2. What is Bioenergy ? 2

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3. What is cDNA ? Name the enzyme responsible for production of cDNA.
2

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4. Briefly write about the rDNA technolog5r. 2

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5. Write down the role of media in cell culture Process. 2

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6. Define the terms 'l^agging, and 'Leading' skands. 2

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7. What is Proteomics ? 2

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8. What are Antibodies ? 2

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9. Define Gene Therapy. 2

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10. What is DNA Probe ? 2

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11. What is micropropagation ? 2

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12. Briefly describe the steps involved in the production of monoclonal
antibodies. 3

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13. What are restriction enzymes ?

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74. Explain the skucture of any oru cloning vector. 3

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1.5. Who developed the PCR process ? Explain the main steps involved in the
PCR technique. 3

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76. What are the transgenic plants ? Explain briefly. J

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77. Explain the structure of IRNA. 5

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18. What is RFLP ? Mention the basic steps. J

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19. Write few applications of microbial culture. J

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20. What is electrophoresis ? Explain. J

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2't. Explain the cDNA library construction process. 5

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32T BTEC 15 ] Cnntd.

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What is ELISA ? Write down the steps of ELISA testing. 2+3=5

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22. Explain the role of microbes as bio-fertilizers. 5

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What is Bioinlormatics ? Write about the uses of bioinformatics tools.

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2j. Write short notes on : (anY ttoo) 2Uzx2=5

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(a) Southern blotting
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(b) rPR
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DNA Helicase

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(d) Transgenic animal.

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