Page 1
Shivaji University , Kolhapur
Seat Paper Code(ENT202017)
No.
M.Sc Entrance Exam , MAY-2020
58722-Biotechnology/Biochemistry/Medical Information Management
Day And Date : Sunday 22/11/2020 Total Marks : 100
Time : 10:30 To 12:00
Marks
1 If an enzyme has optimal pH value of 2, it is most likely present in 1
A) Stomach
B) Deodenum
C) Mouth
D) Gall bladder
2 Haploid plants can be obtained from 1
A) Leaf culture
B) Bud culture
C) Root culture
D) Anther culture
3 [a1, a2, a3] is called a --------- 1
A) row matrix
B) Coloumn matrix
C) determinant
D) database
4 The ovule in which embryo sac become horse shoe shaped in 1
A) Amphitropous
B) Camphylotropous
C) Orthotropous
D) Anatropous
5 Morphine is obtained from 1
A) Aconitum nacelles
B) Papaver somniferum
C) Rauwolffia serpentina
D) Cinchona officinalis
6 In meiosis-I, the Centromere undergoes 1
A) Division between anaphase and Interphase
B) Division between prophase and metaphase
C) Division but the daughter chromosomes do not separate
D) No division
7 Development of shoot and root is determined by 1
A) Cytokinin and auxin ratio
B) Enzymes
C) Temperature
D) Plant nutrients
Page 2
8 Which of the following is used in pencils? 1
A) Graphite
B) Silicon
C) Charcoal
D) Phosphorous
9 Washing soda is the common name for 1
A) Sodium carbonate
B) Calcium bicarbonate
C) Sodium bicarbonate
D) Calcium carbonate
10 Which of the following disinfectant is effective against viruses? 1
A) Formaldehyde
B) Hydrogen peroxide
C) Hypoclorite
D) All of these
11 Which of the following pioneers of Microbiology is credited with the discovery of 1
microorganisms using high quality magnifying lenses (early microscopes)?
A) Anton Van Leeuwenhoek
B) Louis Pasteur
C) Robert Hooke
D) Robert Koch
12 Peptidoglycan is also known as 1
A) N-acetyl muramic acid
B) .murein mucopeptide
C) N acetylglucosamine
D) mesodiaminopimetic acid
13 Which of the following is an example of chemolithoautotroph? 1
A) Sulphur-oxidizing bacteria
B) Hydrogen bacteria
C) Nitrifying bacteria
D) All of the above
14 Which of the following virulence factors of E. coli is important for attachment to 1
host epithelial cells in the
A) Alpha hemolysin
B) Urease
C) . K1 antigen
D) Pili
15 Peptone water and nutrient broth are 1
A) selective media
B) enriched media
C) basal media
D) none of these
16 Which of the following bacteria can grow in alkaline pH? 1
A) Shigella
B) Lactobacilli
C) Salmonella
D) Vibrio cholerae
Page 3
17 virus establishes itself in an animal : 1
A) By killing the animal cells and replacing them with its own genetic material
B) By invading the animal cells and replicating the cell’s genetic material
C) By creating new animal cells
D) By invading the animal cells and replicating its own genetic material
18 Generation time of Escherichia coli is 1
A) 20 minutes
B) 20 hours
C) 200 minutes
D) 20days
19 Light year is a measurement of 1
A) speed of aeroplanes
B) speed of light
C) Stellar distances
D) speed of rockets
20 Among following -----------is well known laboratory organism? 1
A) B. subtilis
B) E. coli
C) S. Typhi
D) HIV
21 Bacterial DNA is not cleaved by its own restriction enzymes because they are ------ 1
---
A) Methylated
B) Deleted
C) Bound by inhibitory protein
D) Not accessible to restriction enzyme.
22 Malaria is caused by -------------- 1
A) Ascaris
B) Foulair
C) Leech
D) Plasmodium
23 In prokaryotes, protein synthesis is carried out by ………………ribosomes 1
A) 30 S
B) 80 S
C) 50 S
D) 70 S
24 The components of an ecosystem are 1
A) Trees and weeds
B) Plants and animals
C) Man and plants
D) Biotic and abiotic
25 Among following ……………….is a high level language 1
A) FORTRAN
B) UNIX
C) FASTA
D) Assembler
Page 4
26 Peptidoglycan layer present in the cell wall of ………….. 1
A) Archaeobacteria
B) Bacteria
C) Fungi
D) Higher plants
27 The first letter code of aspartate amino acid is……… 1
A) d
B) f
C) g
D) e
28 Which of the following is the smallest amino acid 1
A) Glycine
B) Proline
C) Cysteine
D) Serine
29 The medium which allows the growth of more than one microorganisms of interest 1
but with morphologically distinguishable colonies is known as
A) selective medium
B) enrichment medium
C) differential medium
D) none of these
30 Which of the following is a rich source of vitamin B12 1
A) Liver
B) Green leafy vegetables
C) Cheese
D) Citrus fruits
31 Which of the following buffer helps to maintain our body pH? 1
A) Phosphate buffer
B) Carbonate -bicarbonate buffer
C) Acetate buffer
D) Tris buffer
32 A biological polymer contains an imidazole group, a phenyl group and guanido 1
group containing moieties. Which of the following will be the nature of this
molecule
A) Cellulose
B) Protein
C) Cerebrosides
D) Nucleic acids
33 An example of a digestive hormone is 1
A) Gastrin
B) Insulin
C) Pepsin
D) Thyroxin
34 Taxol is an anticancer agent which acts by 1
A) Inhibiting DNA synthesis
B) Intercalating with the DNA
C) Interfering with the mitotic spindle
D) Inhibiting enzymes of DNA synthesis
Page 5
35 Reaction center of photo system-I in green plants is 1
A) P680
B) P690
C) P700
D) P780
36 In nucleoside, nitrogen base is attached to pentose sugar at 1
A) Carbon – 1 of pentose sugar
B) Carbon – 2 of pentose sugar
C) Carbon – 4 of pentose sugar
D) Carbon – 5 of pentose sugar
37 DNA molecules makes a complete turn after every 1
A) 20 Å
B) 34 Å
C) 3.4 Å
D) 10 base pairs
38 Which of the following is not tool of genetic engineering? 1
A) Vectors
B) Enzymes
C) Foreign DNA
D) GMO
39 The most common plasmid vector used in genetic engineering is 1
A) PBR 328
B) PBR 322
C) PBR 325
D) PBR 330
40 Three dimensional shape of tRNA is 1
A) L-shaped
B) Clover leaf-like
C) X-shaped
D) Y-shaped
41 Genome is 1
A) Genes on nuclear DNA
B) Nuclear DNA + mitochondrial DNA
C) Nuclear DNA + chloroplast DNA
D) Nuclear DNA + Mitochondrial DNA + Chloroplast DNA
42 VNTRs represnets 1
A) New terminal regions in DNA
B) Functional genes in the DNA
C) Split genes in the sample DNA
D) Specific non-coding sequences with unique tandem repeats
43 Variations observed during tissue culture of some plants are known as 1
A) Clonal variations
B) Somatic variations
C) Somaclonal variations
D) Tissue culture variations
Page 6
44 Callus is 1
A) Tissue that forms embryo
B) an insoluble carbohydrate
C) Unorganised actively dividing mass of cells maintained in culture
D) Tissue that growth to form embryoid
45 The codons which may present at 3¢ end of mRNA 1
A) UAA
B) UAG
C) UGA
D) Any one of these
46 Double fertilization is found in 1
A) Bryophytes
B) Angiosperms
C) Gymnosperms
D) Pteridophytes
47 DNA fingerprinting is based on 1
A) Occurance of VNTR’s
B) Knowledge of human karyotype
C) Cloned DNA
D) Recombinant DNA
48 Dolly sheep was genetically similar to 1
A) The mother from which nucleated fertilized egg was taken
B) The mother from which nuclear DNA of udder cell was taken
C) The surrogate mother
D) Both surrogate mother and nuclear donor mother
49 The complete set of chromosomal and extrachromosomal genes of an organisms 1
is called
A) Genome
B) Gene pool
C) Gene bank
D) Gene library
50 5.The DNA segment to be cloned is called 1
A) a.Gene segment
B) b.DNA fragment
C) c.DNA insert
D) d.All of these
51 8.Which of the following is not a restriction endonuclease? 1
A) a.Eco RI
B) b.DNA ligase
C) c.Hind III
D) d.Bam H1
52 10.Taq polymerase is used in PCR because of its 1
A) a.Low thermal stability
B) b.High fidelity
C) c.High speed
D) d.High thermal stability
Page 7
53 Nick translation is done by 1
A) a.DNA polymerase I
B) b.DNA polymerase II
C) c.DNA ligase
D) d.Kinase
54 Which of the following is a thermo stable DNA polymerase 1
A) a.Taq polymerase
B) b.Vent polymerase
C) c.Pfu polymerase
D) d.All of these
55 Eco R1 is a 1
A) a.Endonuclease
B) b.Polymerase enzyme
C) c.Ligase
D) d.Exonuclease
56 What is the net yield of ATP in anaerobic glycolysis? 1
A) 36
B) 38
C) 2
D) May vary from time to time
57 The hormone responsible for ripening of fruits is 1
A) Ethylene
B) Cytokinin
C) Auxin
D) ABA
58 Table sugar used by us regularly is 1
A) Sucrose
B) Fructose
C) Dextrose
D) Glucose
59 Which of the following indicates that the pK of an acid is numerically equal to the 1
pH of the solution when the molar concentration of the acid and its conjugate base
are equal?
A) Michaelis Menton's equation
B) Hardy Weinberg equation
C) Henderson-Hasselbalch equation
D) Briggs Haldane's equation
60 Which of the following enzyme is required to release the tension imposed by 1
uncoiling of strands
A) Endonuclease
B) DNA ligase
C) DNA gyrase
D) DNA helicase
61 Ecosystem may be defined as 1
A) A species along with environment
B) Plants found in water
C) Plant found in land
D) All plants and animal species along with environment
Page 8
62 The nodule forming bacteria are 1
A) Azotobacter
B) Nitrobacter
C) Clostridium
D) Rhizobium
63 Pituitary gland is located in......... 1
A) Intestine
B) Liver
C) Kidney
D) Brain
64 Where do primary consumers get their energy? 1
A) directly from the sun
B) eating producers
C) eating animals
D) eating dead things
65 Which of the following statements is true? 1
A) Invertebrates possesses a tubular nerve cord
B) Non-chordates have a vertebral column
C) All chordates are vertebrates
D) All vertebrates are chordates
66 Which of the following reptile has four chambered heart? 1
A) Turtle
B) King cobra
C) Crocodile
D) Sphenodon
67 Fossils are most commonly preserved in..... 1
A) Metamorphic rocks
B) Igneous rocks
C) Sedimentary rocks
D) Any type of rock
68 Select the correct statement 1
A) Legumes are incapable of fixing nitrogen
B) Legumes fix nitrogen through bacteria living in fruits
C) Legumes fix nitrogen only by bacteria present in root nodules
D) None of the above
69 Which organism possesses characteristics of plants and animals? 1
A) Bacteria
B) Monera
C) Euglena
D) Mycoplasma
70 Ambulacral system (Water vascular system) is present in 1
A) Annelides
B) Arthropods
C) Molluscs
D) Echinoderms
Page 9
71 The genotypic ratio of a mono-hybrid cross is......... 1
A) 3:1
B) 1:2:1
C) 2:1:1
D) 9:3:3:1
72 High content of sugar in honey is........... 1
A) Glucose
B) Sucrose
C) Fructose
D) Lactose
73 Sitophilus oryzae is a common stored grain pest of 1
A) Sugar
B) Rice
C) Sugarcane
D) Pulses
74 Mulberry silkworm is....... 1
A) Bombax mori
B) Anthraea mylitta
C) Anthreaea assamensis
D) Attacus altas
75 Animals with chitinous exoskeleton are.... 1
A) Sea-Urchin
B) Insects
C) Snail
D) Sponges
76 Fertilization of ova in human take place in...... 1
A) Overy
B) Vagina
C) Fallopian tube
D) Uterus
77 Which of the following is tapeworm? 1
A) Schistosoma
B) Fasciola
C) Enterobius
D) Taenia
78 The newly hatched tadpole is breaths through its..... 1
A) External gills
B) Internal gills
C) Lungs
D) All of the above
79 Stomata in bryophytes are present in the 1
A) Capsule
B) Leaf
C) Stem
D) Seta
Page 10
80 Bacteriophage are readily counted by the process of: 1
A) Immunoassays
B) . ELISA
C) Plaque assays
D) Tissue cell culture
81 The gram-negative organisms is 1
A) Actinomyces
B) Bacillus
C) Clostridium
D) None of these
82 Hemoglobin is a 1
A) Structural protein
B) Transport protein
C) Storage protein
D) Defense protein
83 The end product of glycolysis on vigorous exercise is 1
A) Pyruvate
B) Acetyl CoA
C) Lactate
D) CO2
84 Electromagnetic radiations with maximum wavelength is_________ 1
A) Ultraviolet
B) Radiowaves
C) X-ray
D) Infrared
85 Which of the following has a negative charge? 1
A) Neutrino
B) Neutron
C) Positron
D) Antiproton
86 The oxidation number of carbon in CH2O is ________. 1
A) -2
B) +2
C) 00
D) +4
87 The strongest hydrogen bond present in _________. 1
A) O-H-----S
B) S-H----O
C) F-H----F
D) F-H----O
88 Who proposed corpuscular theory of light? 1
A) Newton
B) Einstein
C) Rutherford
D) Calvin
Page 11
89 These are vector quantities, except __________. 1
A) Displacement
B) Velocity
C) Accelaration
D) Temperature
90 Development of saprophyte from gametophyte, is called 1
A) Apomixis
B) Apospory
C) Apogamy
D) Diplospory
91 In CGS system, the unit of mass is ________. 1
A) Gram
B) Second
C) Centimeter
D) Kilogram
92 A molal solution is one that contains one mole of the solute in _____. 1
A) 1000 gms of solvent
B) 1000 ml of solvent
C) 1000 ml of solution
D) 22.4 lit of solution
93 The unit of activation energy is/are __________. 1
A) Kg
B) Kg/mole
C) KJ/K
D) KJ/K.mole
94 The copper is mainly extracted from ___________. 1
A) Cuprite
B) Azurite
C) Copper pyrites
D) Malachite
95 Human Genome Project was completed in ------------ 1
A) 2003
B) 1999
C) 1973
D) 2000
96 A -------- is a set of instructions executed by the computer 1
A) Program
B) Data
C) Output
D) None of these
97 Among following --------- is a browser program. 1
A) MS Word
B) Database Program
C) Netscase
D) Help Program
Page 12
98 1 kb corresponds to ……….bytes 1
A) 1024
B) 1000
C) 10
D) 2000
99 2.Integrated circuits are used in ...............generation of computers. 1
A) a.third
B) b.first
C) c.second
D) d.None of the above
100 Which blood group is called the universal donor ? 1
A) A
B) B
C) O
D) AB