HBSE Class 12 Question Paper 2024 Biotechnology

Haryana Board

QUESTIONPDF

PAPERS
2024

HBSE PYQP

CLASS : 12th (Sr. Secondary) Code No. 1233
Series : SS/Annual Exam.-2024
Roll No.

tSo izkS|ksfxdh
BIOTECHNOLOGY
[ Hindi and English Medium ]
ACADEMIC/OPEN

(Only for Fresh/Re-appear/Improvement/Additional Candidates)

Time allowed : 2:30 hours ] [ Maximum Marks : 60
• Ñi;k tk¡p dj ysa fd bl iz'u&i= esa eqfnzr i`"B 16 rFkk iz'u 16 gSaA
Please make sure that the printed pages in this question paper are 16 in number
and it contains 16 questions.

• iz'u&i= esa nkfgus gkFk dh vksj fn;s x;s dksM uEcj dks Nk= mÙkj&iqfLrdk ds eq[;&i`"B ij fy[ksaA
The Code No. on the right side of the question paper should be written by the
candidate on the front page of the answer-book.

• Ñi;k iz'u dk mÙkj fy[kuk 'kq: djus ls igys] iz'u dk Øekad vo'; fy[ksaA
Before beginning to answer a question, its Serial Number must be written.

• mÙkj&iqfLrdk ds chp esa [kkyh iUuk@iUus u NksMas+A
Don’t leave blank page/pages in your answer-book.

• mÙkj&iqfLrdk ds vfrfjDr dksbZ vU; 'khV ugha feysxhA vr% vko';drkuqlkj gh fy[ksa vkSj fy[kk mÙkj u
dkVsaA
Except answer-book, no extra sheet will be given. Write to the point and do not
strike the written answer.

1233 P. T. O.

(2) 1233
• ijh{kkFkhZ viuk jksy ua0 iz'u&i= ij vo'; fy[ksaA jksy ua0 ds vfrfjDr iz'u&i= ij vU; dqN Hkh u
fy[ksa vkSj oSdfYid iz'uksa ds mÙkjksa ij fdlh izdkj dk fu'kku u yxk,¡A
Candidates must write their Roll No. on the question paper. Except Roll No. do not
write anything on question paper and don't make any mark on answers of objective
type questions.

• d`i;k iz'uksa ds mÙkj nsus lss iwoZ ;g lqfuf'pr dj ysa fd iz'u&i= iw.kZ o lgh gS] ijh{kk ds mijkUr bl
lEcU/k esa dksbZ Hkh nkok Lohdkj ugha fd;k tk;sxkA
Before answering the questions, ensure that you have been supplied the correct and
complete question paper, no claim in this regard, will be entertained after
examination.

lkekU; funsZ'k %
(i) bl iz'u&i= esa dqy 16 iz'u gSa] ftUgsa pkj [k.Mksa % v] c]
c] l vkSj n esa ck¡Vk x;k gSA lHkh iz'u
vfuok;Z gSaA

(ii) [k.M & v ds iz'u la[;k 1 esa iUnzg (i-xv) oLrqfu"B iz'u gSa] ftlesa iz'u (i) ls (vi) rd
cgqfodYih; iz'u gSa] iz'u (vii) ls (ix) rd fjDr LFkku dh iwfrZ okys ç'u gSa] iz'u (x) ls (xii) rd
izR;sd iz'u dk mÙkj ,d ;k nks okD;ksa esa nhft,A (xiii) ls (xv) rd vfHkdFku (A) vkSj dkj.k (R)
okys ç'u gSaA çR;sd ç'u 1 vad dk gSA
(iii) [k.M & c esa iz'u la[;k 2 ls 7 rd vfry?kq mÙkjh; iz'u gSaA çR;sd ç'u 2 vadksa dk gSA
(iv) [k.M & l esa ç'u la[;k 8 ls 13 rd y?kq mÙkjh; iz'u gSaA çR;sd ç'u 3 vadksa dk gSA
(v) [k.M & n esa ç'u la[;k 14 ls 16 rd nh?kZ mÙkjh; iz'u gSaA çR;sd ç'u 5 vadksa dk gSA
(vi) ç'u&i= esa lexz:i ls dksbZ fodYi ugha gS ysfdu [k.M & c ds nks ç'uksa ¼6 ,oa 7½] [k.M & l
ds nks ç'uksa ¼12 ,oa 13½ rFkk [k.M & n ds lHkh iz'uksa (14, 15 ,oa 16) esa vkarfjd fodYi
fn, x, gSAa ,sls iz'uksa esa ls vkidks fn;s x;s fodYiksa esa ls dsoy ,d gh iz'u djuk gSA

1233

(3) 1233
General Instructions :
(i) This question-paper consist of 16 questions, which are divided into four
Sections : A, B, C and D. All questions are compulsory.
(ii) Question No. 1 of Section – A has fifteen (i-xv) objective type questions in
which questions from (i) to (vi) are multiple choice type questions, (vii) to (ix)
are filling the blanks type questions, the answer of each question from (x) to
(xii) may be given in one or two sentences and (xiii) to (xv) are Assertion (A)
and Reason (R) type questions. Each question carries 1 mark.
(iii) Question Nos. 2 to 7 of Section – B are very short answer type questions.
Each question carries 2 marks.
(iv) Question Nos. 8 to 13 of Section – C are short answer type questions. Each
question carries 3 marks.
(v) Question Nos. 14 to 16 of Section – D are long answer type questions. Each
question carries 5 marks.

(vi) There is no overall choice but in two questions (6 & 7) of Section – B, two
questions (12 & 13) of Section – C and all questions (14, 15 & 16) of
Section – D internal choice are given. You have to attempt only one of the
given choice in such questions.

[k.M – v
SECTION – A
¼oLrqfu"B iz'u½
(Objective Type Questions)

1. (i) dkbeksflu ,atkbe fdl m|ksx esa ç;qDr gksrk gS \ 1

(A) lkcqu (B) iuhj
(C) is; (D) peM+k
1233 P. T. O.

(4) 1233
Chymosin enzyme is used in which Industry ?

(A) Soap

(B) Cheese

(C) Beverage

(D) Leather

(ii) czkapM~ psu vehuks vEy dkSu-lk gS \ 1

(A) Y;wlhu (B) osyhu

(C) ykblhu (D) ;s lHkh

Which is a branched chain amino acid ?

(A) Leucine (B) Valine

(C) Lysine (D) All of these

(iii) ,d ysVj dksM 'G' fdl vehuks vEy dk gS \ 1

(A) XywVkfeu

(B) XywVkfed vEy

(C) Xykblhu

(D) buesa ls dksbZ ugha

1233

(5) 1233
One letter code 'G' is stands for which amino acid ?

(A) Glutamine

(B) Glutamic acid

(C) Glycine

(D) None of these

(iv) Y;wdksukWLVkWd ehlsUVsjkW;M fdlds mRikn ds fy, ç;ksx gksrk gS \ 1

(A) bFksukWy

(B) LVªsIVksekbflu

(C) MsDLVªku

(D) ,ekbyst

Leuconostoc mesenteroides is used to produce :

(A) Ethanol

(B) Streptomycin

(C) Dextran

(D) Amylase

1233 P. T. O.

(6) 1233
(v) fuEufyf[kr esa ls dkSu-lk xq.k LdksiksySfeu dk gS \ 1

(A) ,UVhQfVZfyVh

(B) ,UVhgkbijVsfUlo

(C) ,UVhcSDVhfj;y

(D) ,UVheysfj;k
Scopolamine have which of the following property ?

(A) Antifertility

(B) Antihypertensive

(C) Antibacterial

(D) Antimalaria

(vi) bfjFkzksiks;fVu dh esfMdy ,sfIyds'ku fdlesa gS \ 1

(A) ,uhfe;k

(B) gkVZ vVSd
(C) buQfVZfyVh

(D) fldy lsy ,uhfe;k

1233

(7) 1233
Erythropoietin have medical application in :

(A) Anaemia

(B) Heart attack

(C) Infertility

(D) Sickle cell anaemia

(vii) 'kdZjk xzqi ds tksM+us ¼,Mh'ku½ dks ------------- dgrs gSaA 1

Addition of sugar group is called as …………. .

(viii) dikl esa thu ukbfVªysl ------------- ds fu;a=.k ds fy, çfo"V fd;k tkrk gSA 1

The gene nitrilase is introduced in cotton to control …………. .

(ix) ih lh vkj dk vkfo"dkj ewy:i ls ------------- us 1985 esa fd;kA 1

PCR was originally invented by …………. in 1985.

(x) m|ksx esa dkSu-lk lw{etho flfVªd vEy ds mRiknu esa ç;ksx fd;k tkrk gS \ 1

Which microorganism is used to produce citric acid in Industry ?

1233 P. T. O.

(8) 1233
(xi) Dyksfuax osDVj dk dk;Z djus okys dkWlfeM dk bulVZ lkbt (Kb) D;k gksrk gS \ 1

What is the insert size (Kb) of cosmid used as cloning vector ?

(xii) baVjQhjksu vYQk fdl jksx ds mipkj esa ç;qDr gksrk gS \ 1

Interferon alpha is used for the cure of which disease ?

uhps fn, x, ç'u la[;k (xiii), (xiv) ,oa (xv) esa nks-nks dFku fn, x, gSa % vfHkdFku (A) vkSj dkj.k
(R)A ç'uksa ds uhps fn, x, mi;qDr fodYi dk p;u djrs gq, mÙkj nhft,A

The questions (xiii), (xiv) and (xv) given below consists of two statements each :
Assertion (A) and Reason (R). Answer the questions by selecting the
appropriate option given below.

(xiii) vfHkdFku (A) : tsusfVd bathfu;fjax dks iqu% la;kstd Mh ,u , rduhd Hkh dgrs gSaA 1

dkj.k (R) : ;g fdlh tho dh vkuqoaf'kd lajpuk esa lq/kkj ykrk gSA
fodYi %
(A) vfHkdFku (A) rFkk dkj.k (R) nksuksa lR; gSa rFkk dkj.k (R), vfHkdFku (A) dh lgh O;k[;k
gSA

(B) vfHkdFku (A) rFkk dkj.k (R) nksuksa lR; gSa] ijUrq dkj.k (R), vfHkdFku (A) dh lgh O;k[;k
ugha gSA

(C) vfHkdFku (A) lR; gS] ijUrq dkj.k (R) vlR; gSA

(D) vfHkdFku (A) vkSj dkj.k (R) nksuksa vlR; gSaA
1233

(9) 1233
Assertion (A) : Genetic Engineering is also called recombinant DNA
technique.

Reason (R) : It brings about improvement of genetic make up of an
organism.

Options :

(A) Both Assertion (A) and Reason (R) are true and Reason (R) is the
correct explanation of Assertion (A).

(B) Both Assertion (A) and Reason (R) are true, but Reason (R) is not the
correct explanation of Assertion (A).

(C) Assertion (A) is true, but Reason (R) is false.

(D) Both Assertion (A) and Reason (R) are false.

(xiv) vfHkdFku (A) : dksMhu ,d ,uSYtsfld gSA 1

dkj.k (R) : bl ikS/ks dk òksr iSisoj Lih'kht+ gSA
fodYi %
(A) vfHkdFku (A) rFkk dkj.k (R) nksuksa lR; gSa rFkk dkj.k (R), vfHkdFku (A) dh lgh O;k[;k
gSA
(B) vfHkdFku (A) rFkk dkj.k (R) nksuksa lR; gSa] ijUrq dkj.k (R), vfHkdFku (A) dh lgh O;k[;k
ugha gSA
(C) vfHkdFku (A) lR; gS] ijUrq dkj.k (R) vlR; gSA
(D) vfHkdFku (A) vkSj dkj.k (R) nksuksa vlR; gSaA

1233 P. T. O.

( 10 ) 1233
Assertion (A) : Codeine is an analgesic.

Reason (R) : The plant source of it is papaver species.

Options :

(A) Both Assertion (A) and Reason (R) are true and Reason (R) is the
correct explanation of Assertion (A).

(B) Both Assertion (A) and Reason (R) are true, but Reason (R) is not the
correct explanation of Assertion (A).

(C) Assertion (A) is true, but Reason (R) is false.

(D) Both Assertion (A) and Reason (R) are false.

(xv) vfHkdFku (A) : LVsfjykbts'ku lw{ethoksa ij dkcw ikus esa lgk;rk djrk gSA 1

dkj.k (R) : ,DlIykaV vkWVksDysOM fd, tkrs gSaA

fodYi %
(A) vfHkdFku (A) rFkk dkj.k (R) nksuksa lR; gSa rFkk dkj.k (R), vfHkdFku (A) dh lgh O;k[;k
gSA
(B) vfHkdFku (A) rFkk dkj.k (R) nksuksa lR; gSa] ysfdu dkj.k (R), vfHkdFku (A) dh lgh
O;k[;k ugha gSA
(C) vfHkdFku (A) lR; gS] ijUrq dkj.k (R) vlR; gSA
(D) vfHkdFku (A) vkSj dkj.k (R) nksuksa vlR; gSaA
1233

( 11 ) 1233
Assertion (A) : Sterilisation helps to overcome microbes.

Reason (R) : Explants are autoclaved.

Options :

(A) Both Assertion (A) and Reason (R) are true and Reason (R) is the
correct explanation of Assertion (A).

(B) Both Assertion (A) and Reason (R) are true, but Reason (R) is not the
correct explanation of Assertion (A).

(C) Assertion (A) is true, but Reason (R) is false.

(D) Both Assertion (A) and Reason (R) are false.

[k.M – c

SECTION – B

¼vfry?kq mÙkjh; iz'u½

(Very Short Answer Type Questions)

2. gekjs 'kjhj esa XywVkfFkvksu dh Hkwfedk D;k gS \ 2

What is the role of glutathione in our body ?

1233 P. T. O.

( 12 ) 1233
3. Hind III ,atkbe ds ekbØksfc;y òksr] fjdfXu'ku ,oa Dyhost ¼fonyu½ lkbV fyf[k,A 2

Write down microbial source, recognition and cleavage site of enzyme Hind III.

4. vkWxsZusyh Mh ,u , ds ckjs esa fyf[k,A 2

Write about organelle DNA.

5. ekbØksfc;y dYpj rduhd esa dkSu-ls fofHkUu ,aVhQkse ç;ksx esa yk, tkrs gSa \ 2

What are various antifoams used in microbial culture technique ?

6. okafNr ekbØksfc;y mRiknksa ¼çksMDV½ ds vyxko ¼vkblksys'ku½ esa 'kkfey fofHkUu pj.kksa dks fyf[k,A 2

Write various steps involved in isolation of the desired microbial products.

vFkok
OR

cSDVhfj;k] ;hLV] QQ¡wnh vkSj ok;jl dh lw{ethoh o`f) dk lkekU; çk:i fyf[k,A

Write down the general pattern of microbial growth of bacteria, yeast, fungi &
virus.

7. Øk;ksfçtosZ'ku fdl çdkj dh tkrh gS \ 2
How is cryopreservation done ?

1233

( 13 ) 1233
vFkok
OR

tUrq dksf'kdk dYpj esa lhje dh Hkwfedk D;k gksrh gS \
What is the role of serum in animal cell culture ?

[k.M – l

SECTION – C

¼y?kq mÙkjh; iz'u½

(Short Answer Type Questions)

8. Dyksfuax esa Mh ,u , ykbxst dh Hkwfedk fyf[k,A 3

Write down the role of DNA ligase in cloning.

9. dk;kZRed ¼QaD'kuy½ çksfV;ksfeDl ds ckjs esa fyf[k,A 3

Write about functional proteomics.

10. QsM cSp dYpj D;k gS \ o.kZu dhft,A 3

What is fed batch culture ? Explain.

11. buoVsZM ekbØksLdksi ds dk;Z D;k gSa \ ;g fdl çdkj dk;Z djrk gS \ 3

What is the function of inverted microscope ? How does it works ?

1233 P. T. O.

( 14 ) 1233
12. Vªkaltsfud Qlyksa esa dhV vojks/kdrk iSnk djus ds fy, vkuqoaf'kd bathfu;fjax vk/kkfjr fof/k;ksa dks
fyf[k,A 3

Write down the genetic engineering strategies to create insect resistance in
transgenic crops.

vFkok
vFkok
OR

ikS/kksa esa tsusfVd bathfu;fjax }kjk esy LVsfjfyVh fdl çdkj iSnk dh tkrh gS \

How is male sterility created in plants by genetic engineering strategies ?

13. ekbØksçksisxs'ku fof/k esa fofHkUu ifjHkkf"kr pj.kksa dks fyf[k,A 3

Write down the various defined steps in micro-propagation method.

vFkok

OR

tsusfVd bathfu;fjax }kjk chtksa esa çksVhu dh xq.koÙkk fdl çdkj lq/kkjh tk ldrh gS \

How can seed protein quality be improved by Genetic Engineering ?

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( 15 ) 1233
[k.M – n
SECTION – D
¼fuca/kkRed iz'u½
(Essay Type Questions)

14. iqu% la;kstd IykfTeM dh mifLFkfr ds fy, LØhfuax dh uhyh-lQsn p;u fof/k dk o.kZu dhft,A 5
Describe the blue-white selection method of screening for the presence of
recombinant plasmid.

vFkok
OR

iqu% la;kstd Mh ,u , dks gksLV dksf'kdk ¼lsy½ esa iqj%LFkkfir ¼baVªksM;~ wLM½ dj ldus ds fy, fofHkUu
fof/k;ksa dk o.kZu dhft,A

Describe the various techniques by which recombinant DNA can be introduced
into host cell.

15. ,d thukse dh iw.kZ:i ls flDosfUlax djus ds D;k dkj.k gSa \ 5

What are the reasons for completely sequencing a genome ?

vFkok
OR

Mh ,u , ekbØks,sjs çkS|ksfxdh dk fl)kUr D;k gS \ bldh mi;ksfxrk fyf[k,A
What is the principle of DNA Microarray Technology ? Write its utility.

1233 P. T. O.

( 16 ) 1233
16. tarq dksf'kdk lao/kZu esa pH dh Hkwfedk dk o.kZu dhft,A 5

Describe the role of pH in culturing animal cells.

vFkok

OR

Hkzw.k LVse lsy çkS|ksfxdh ds laHkkfor ykHkksa dk o.kZu dhft,A
Describe the possible benefits of Embryonic stem cell technology.

S

1233

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