HBSE Class 12 Question Paper 2025 Bio Technology

CLASS : 12th (Sr. Secondary) Code No. 2233
Series : SS/Annual Exam.-2025
Roll No.

tSo izkS|ksfxdh
BIOTECHNOLOGY
[ Hindi and English Medium ]
ACADEMIC/OPEN

(Only for Fresh/Re-appear/Improvement/Additional Candidates)

Time allowed : 2:30 hours ] [ Maximum Marks : 60
• Ñi;k tk¡p dj ysa fd bl iz'u&i= esa eqfnzr i`"B 16 rFkk iz'u 30 gSaA
Please make sure that the printed pages in this question paper are 16 in number
and it contains 30 questions.

• iz'u&i= esa nkfgus gkFk dh vksj fn;s x;s dksM uEcj dks Nk= mÙkj&iqfLrdk ds eq[;&i`"B ij fy[ksaA
The Code No. on the right side of the question paper should be written by the
candidate on the front page of the answer-book.

• Ñi;k iz'u dk mÙkj fy[kuk 'kq: djus ls igys] iz'u dk Øekad vo'; fy[ksaA
Before beginning to answer a question, its Serial Number must be written.

• mÙkj&iqfLrdk ds chp esa [kkyh iUuk@iUus u NksMsa+A
Don’t leave blank page/pages in your answer-book.

• mÙkj&iqfLrdk ds vfrfjDr dksbZ vU; 'khV ugha feysxhA vr% vko';drkuqlkj gh fy[ksa vkSj fy[kk mÙkj u
dkVsaA
Except answer-book, no extra sheet will be given. Write to the point and do not
strike the written answer.

2233 P. T. O.

(2) 2233
• ijh{kkFkhZ viuk jksy ua0 iz'u&i= ij vo'; fy[ksaA jksy ua0 ds vfrfjDr iz'u&i= ij vU; dqN Hkh u
fy[ksa vkSj oSdfYid iz'uksa ds mÙkjksa ij fdlh izdkj dk fu'kku u yxk,¡A
Candidates must write their Roll No. on the question paper. Except Roll No. do not
write anything on question paper and don't make any mark on answers of objective
type questions.

• d`i;k iz'uksa ds mÙkj nsus lss iwoZ ;g lqfuf'pr dj ysa fd iz'u&i= iw.kZ o lgh gS] ijh{kk ds mijkUr bl
lEcU/k esa dksbZ Hkh nkok Lohdkj ugha fd;k tk;sxkA
Before answering the questions, ensure that you have been supplied the correct and
complete question paper, no claim in this regard, will be entertained after
examination.

lkekU; funsZ'k %
(i) bl iz'u&i= esa dqy 30 iz'u gSa] ftUgsa pkj [k.Mksa % v] c]
c] l vkSj n esa ck¡Vk x;k gSA lHkh iz'u
vfuok;Z gSaA

(ii) [k.M & v esa 1 ls 15 rd oLrqfu"B izdkj ds iz'u gSa] ftlesa 1 ls 6 rd cgqfodYih; izdkj ds
iz'u gSaA izR;sd iz'u esa pkj fodYi gSa] ftuesa ls lgh fodYi pqus]a 7 ls 9 rd ds iz'u fjDr
LFkku iwfrZ izdkj ds gSa vkSj Nk=ksa dks lgh 'kCn/'kCnksa dks Hkjuk gS] 10 ls 12 rd ds izR;sd iz'u
dk mÙkj ,d ;k nks 'kCnksa esa fn;k tk ldrk gS vkSj 13 ls 15 rd ds iz'u vfHkdFku/dkj.k
izdkj ds gSa vkSj izR;sd iz'u esa pkj fodYi gS]a ftuesa ls lgh fodYi pqusaA izR;sd iz'u 1 vad dk
gSA
(iii) [k.M & c esa iz'u la[;k 16 ls 21 rd vfry?kq mÙkjh; iz'u gSaA izR;sd iz'u 2 vadksa dk gSA
(iv) [k.M & l esa ç'u la[;k 22 ls 27 rd y?kq mÙkjh; iz'u gSaA çR;sd ç'u 3 vadksa dk gSA
(v) [k.M & n esa ç'u la[;k 28 ls 30 rd nh?kZ mÙkjh; iz'u gSaA çR;sd ç'u 5 vadksa dk gSA

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(3) 2233
(vi) ç'u&i= esa lexz:i ls dksbZ fodYi ugha gS] ysfdu [k.M & c ds nks ç'uksa ¼18 ,oa 21½]
[k.M & l ds nks ç'uksa ¼23 ,oa 27½ rFkk [k.M - n ds lHkh iz'uksa ¼28, 29, ,oa 30½ esa
vkarfjd fodYi fn, x, gSaA bl izdkj ds iz'uksa esa ls vkidks fn;s x;s fodYiksa esa ls dsoy ,d gh dk
iz;kl djuk gSA
General Instructions :
(i) This question-paper consist of 30 questions, which are divided into four
Sections : A, B, C and D. All questions are compulsory.
(ii) Section – A has 1 to 15 objective type questions in which questions from 1 to
6 are multiple choice type questions. Each question carries four options, from
which choose correct option, questions from 7 to 9 are fill in the blanks type
questions and students have to fill correct word/words, the answer of each
questions from 10 to 12 may be given in one or two words and questions
from 13 to 15 are Assertion/Reason type and each question carries four
options, from which choose correct option. Each question carries 1 mark.

(iii) Question Nos. 16 to 21 of Section – B are very short answer type questions.
Each question carries 2 marks.
(iv) Question Nos. 22 to 27 of Section – C are short answer type questions.
Each question carries 3 marks.
(v) Question Nos. 28 to 30 of Section – D are long answer type questions. Each
question carries 5 marks.

(vi) There is no overall choice, but in two questions (18 & 21) of Section- B, two
questions (23 & 27) of Section – C, and all questions. (28, 29 & 30) of
Section - D internal choice are given. You have to attempt only one of the
given choices in such questions.

2233 P. T. O.

(4) 2233
[k.M – v
SECTION – A
¼oLrqfu"B iz'u½
(Objective Type Questions)

1. ml ikS/ks dk uke crkb, ftls gekjs oSKkfudksa us dhV izfrjks/kh cuk;k gS % 1

(A) eDdk (B) xsgw¡
(C) dikl ¼chVh-dikl½ (D) pkoy
Name the plant that was made insect resistant by our scientists :

(A) Corn (B) Wheat

(C) Cotton (Bt-cotton) (D) Rice

2. lcls igys fdlus iqf"V dh fd fldy lsy gheksXyksfcu ,d vkosf'kr vehuks ,flM ds dkj.k lkekU;
gheksXyksfcu ls fHkUu gksrk gS \ 1

(A) eSDl is#V~t+

(B) fyul ikWfyax

(C) oh0 ,e0 buxzke

(D) vYcVZ vkbaLVhu

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(5) 2233
Who was the first to confirm that sickle cell haemoglobin differs from normal
haemoglobin due to a charged amino acid ?

(A) Max Perutz

(B) Linus Pauling

(C) V. M. Ingram

(D) Albert Einstein

3. ,d gh thukse ds Hkhrj thu nksgjko ds dkj.k leku vuqØeksa ds fy, fdl 'kCn dk mi;ksx fd;k tkrk
gS \ 1

(A) vkWFkksZykWXl

(B) iSjkykWXl

(C) gkseksykWXl

(D) vkblks,atkbEl

What term is used for sequences that are similar due to gene duplication within
the same genome ?

(A) Orthologs (B) Paralogs

(C) Homologs (D) Isoenzymes

2233 P. T. O.

(6) 2233
4. fuEufyf[kr esa ls dkSu-lk lao/kZu laxzg dsanz ;w0 ds0 (United Kingdom) esa fLFkr gS \ 1

(A) ATCC (B) MTCC

(C) NCIB (D) DSM

Which of the following culture collection centres is located in the U. K. (United
Kingdom) ?

(A) ATCC (B) MTCC

(C) NCIB (D) DSM

5. ^IykaV fV'kw dYpj dk tud* fdls ekuk tkrk gS \ 1

(A) 'oku

(B) 'ysMsu
(C) xksVfyc gScjySaMV~
(D) yqbZ ik'pj
Who is regarded as the 'Father of Plant Tissue Culture' ?

(A) Schwann

(B) Schleiden

(C) Gottlieb Haberlandt

(D) Louis Pasteur

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(7) 2233
6. eksuksDyksuy ,aVhckWMh (mAbs) fo'ks"k :i ls D;k djus ds fy, fMt+kbu fd, x, gSa \ 1

(A) ,aVhtu ds dbZ ,fiVksi ls tqM+uk

(B) ,aVhtu ds ,d ,fiVksi ls fo'ks"k :i ls tqM+uk

(C) xSj-fof'k"V rjhds ls ,aVhtu ls tqM+uk

(D) ,aVhckWMh mRiknu dks jksduk
What are monoclonal antibodies (mAbs) specifically designed to do ?

(A) Bind to multiple epitopes of an antigen

(B) Bind specifically to a single epitope of an antigen

(C) Bind to antigens in a non-specific manner

(D) Inhibit antibody production

7. Mh ,u , ykbxst vklUu U;wfDy;ksVkbM~l ds chp QkWLQksMkbLVj ckW.M cukrk gS vkSj Mh ,u , ds nks

VqdM+ksa dks lgla;kstd :i ls tksM+rk gSA izfrfØ;k ds fy, ,d VqdM+s esa ………….. lewg vkSj nwljs esa

………….. lewg gksuk vko';d gSA 1
+ 1 =1
2 2

DNA ligase forms phosphodiester bonds between adjacent nucleotides and

covalently links two fragments of DNA. The reaction requires one fragment to

have a …………… group and the other a ……………… group.

2233 P. T. O.

(8) 2233
8. dkbeksfVªfIlu ………….. esa la'ysf"kr gksrk gS vkSj vXuk'k;h okfguh ds ek/;e ls xzg.kh esa NksM+k tkrk
gSA 1

Chymotrypsin is synthesized in the …………… and released into the duodenum

through the pancreatic duct.

9. ,.MksLieZ ,d ………….. Ård gS vkSj ,.MksLieZ Ård ds lao/kZu }kjk fVªiyksbM ikS/kksa dk mRiknu
djus ds fy, ,d mRÑ"V lkexzh gSA 1

The endosperm is a …………. tissue and an excellent material to produce triploid

plants by culturing endosperm tissue.

10. fdl thok.kq dk lEiw.kZ thukse loZizFke vuqØfer fd;k x;k Fkk \ 1

Which bacterium was the first to have its whole genome sequenced ?

11. izksVhu ds lzksr ds :i esa lEiw.kZ lw{ethoksa ds mi;ksx ds fy, D;k 'kCn gS \ 1

What is the term for the use of whole microorganisms as a source of protein ?

12. bZ ,l (ES) dksf'kdk,¡ dgk¡ ls izkIr gksrh gSa \ 1

Where are ES cells derived from ?

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(9) 2233
13. vfHkdFku (A) % mRifjorZu ;kn`fPNd gksrs gSa vkSj LokHkkfod :i ls gksrs gSa] ftlls izksVhu lajpuk ;k
dk;Z esa ifjorZu gksrk gSA
dkj.k (R) % lkbV-funsZf'kr mRifjorZu ,d ,slh rduhd gS tks fu;af=r] fof'k"V mRifjorZu dks thu

vuqØe esa is'k djus dh vuqefr nsrh gSA

fodYi % 1

(A) vfHkdFku (A) lgh gS] ysfdu dkj.k (R) xyr gSA
(B) vfHkdFku (A) xyr gS] ysfdu dkj.k (R) lgh gSA
(C) vfHkdFku (A) rFkk dkj.k (R) nksuksa lgh gSa] ysfdu dkj.k (R), vfHkdFku (A) dh lgh O;k[;k ugha
gSA
(D) vfHkdFku (A) rFkk dkj.k (R) nksuksa lgh gSa rFkk dkj.k (R)] vfHkdFku (A) dh lgh O;k[;k gSA

Assertion (A) : Mutations are random and occur naturally, leading to changes
in Protein structure or function.
Reason (R) : Site-directed mutagenesis is a technique that allows for
controlled, specific mutations to be introduced into a gene
sequence.
Options :
(A) Assertion (A) is true, but Reason (R) is false.
(B) Assertion (A) is false, but Reason (R) is true.
(C) Both Assertion (A) and Reason (R) are true, but Reason (R) is not correct
explanation of Assertion (A).
(D) Both Assertion (A) and Reason (R) are true and Reason (R) is the correct
explanation of Assertion (A).

2233 P. T. O.

( 10 ) 2233
14. vfHkdFku (A) : fd.ou izfØ;kvksa esa gok dks thok.kqjfgr djus ds fy, fQYVj LVsfjykbts+'ku dk mi;ksx
fd;k tkrk gSA
dkj.k (R) : fd.ou izfØ;kvksa esa mi;ksx dh tkus okyh gok dk thok.kqjfgr gksuk vko';d ugha gS
D;ksafd ;g lw{ethoksa dks izHkkfor ugha djrh gSA
fodYi % 1

(A) vfHkdFku (A) lgh gS] ysfdu dkj.k (R) xyr gSA

(B) vfHkdFku (A) xyr gS] ysfdu dkj.k (R) lgh gSA

(C) vfHkdFku (A) rFkk dkj.k (R) nksuksa lgh gSa] ysfdu dkj.k (R), vfHkdFku (A) dh lgh O;k[;k ugha
gSA

(D) vfHkdFku (A) rFkk dkj.k (R) nksuksa lgh gSa rFkk dkj.k (R), vfHkdFku (A) dh lgh O;k[;k gSA

Assertion (A) : Filter sterilization is used to sterilize the air in fermentation
processes.
Reason (R) : The air used in fermentation processes is not required to be
sterile as it does not affect the microorganisms.
Option :
(A) Assertion (A) is true, but Reason (R) is false.
(B) Assertion (A) is false, but Reason (R) is true.
(C) Both Assertion (A) and Reason (R) are true, but Reason (R) is not correct
explanation of Assertion (A).
(D) Both Assertion (A) and Reason (R) are true and Reason (R) is the correct
explanation of Assertion (A).

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( 11 ) 2233
15. vfHkdFku (A) : eksuksDyksuy ,aVhckWMh (mAbs) dk mi;ksx uSnkfud ijh{k.kksa esa fof'k"V ,aVhtu dk irk
yxkus ds fy, fd;k tkrk gSA
dkj.k (R) : eksuksDyksuy ,aVhckWMh ,aVhtu-lfØ; ch (B) dksf'kdkvksa dks ek;yksek dksf'kdkvksa ds lkFk
tksM+dj mRikfnr fd;k tkrk gS] tks mUgsa vfuf'pr dky rd c<+us dh vuqefr nsrk gSA
fodYi % 1

(A) vfHkdFku (A) lgh gS] ysfdu dkj.k (R) xyr gSA

(B) vfHkdFku (A) xyr gS] ysfdu dkj.k (R) lgh gSA

(C) vfHkdFku (A) rFkk dkj.k (R) nksuksa lgh gSa] ysfdu dkj.k (R), vfHkdFku (A) dh lgh O;k[;k ugha
gSA

(D) vfHkdFku (A) rFkk dkj.k (R) nksuksa lgh gSa rFkk dkj.k (R), vfHkdFku (A) dh lgh O;k[;k gSA

Assertion (A) : Monoclonal antibodies (mAbs) are used to detect specific
antigens in diagnostic tests.

Reason (R) : Monoclonal antibodies are produced by fusing antigen-activated B
cells with myeloma cells, which allows them to grow indefinitely.

(A) Assertion (A) is true, but Reason (R) is false.

(B) Assertion (A) is false, but Reason (R) is true.

(C) Both Assertion (A) and Reason (R) are true, but Reason (R) is not the
correct explanation of Assertion (A).

(D) Both Assertion (A) and Reason (R) are true and Reason (R) is the correct
explanation of Assertion (A).

2233 P. T. O.

( 12 ) 2233
[k.M – c
SECTION – B

¼vfry?kq mÙkjh; iz'u½
(Short Answer Type Questions)

16. ,xjkst+ tsy ls Mh ,u , VqdM+ksa dks f>Yyh esa LFkkukarfjr djus dh izfØ;k D;k gS \ 2

What is the process of transferring DNA fragments from an agarose gel to a
membrane ?

17. izksVhu dks l?ku lajpukvksa esa eksM+us ds fy, izsfjr djus okyk eq[; cy D;k gS \ 2

What is the main force driving proteins to fold into compact structures ?

18. ,l ,u ih D;k gS \ fdlh O;fDr dks fof'k"V cukus ds fy, Mh ,u , esa fdruk vUrj i;kZIr gS \ 2
What is SNP ? How much amount of difference in DNA is enough to make an
individual unique ?
vFkok
OR

vkj Mh Vh ¼jhdkWEchusaV Mh ,u , VsDuksykWth½ ds pkj cqfu;knh pj.k fyf[k,A 2

Write down the four basic steps of RDT (Recombinant DNA Technology).

19. lw{etho lEo/kZ esa lka'ysf"kd ehfM;k ls vki D;k le>rs gSa \ le>kb,A 2

What do you mean by synthetic media in microbial culture ? Explain.

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20. ck;ksfMxzsMscy IykfLVd D;k gS \ bldk jklk;fud uke fyf[k,A blds mRiknu esa 'kkfey cSDVhfj;k dk
uke crkb, vkSj bldh eq[; [kkeh ds ckjs esa crkb,A 1
2
+ 1 + 1 + 1 =2
2 2 2

What is Biodegradable Plastic ? Write down its chemical name. Name the
bacteria involved in its production and tell about its main drawback.

21. fpfdRlh; mAb-glsZfIVu D;k gS \ blds dk;ks± dh O;k[;k djsaA 2
What is Therapeutic mAb-Herceptin ? Explain its functions.
vFkok
OR
izksVhu lajpuk fuekZ.k esa fdrus izdkj ds vlgla;kstd ca/k lfEefyr gksrs gSa \ muds uke fyf[k,A 2
How many categories of non-covalent bonds are involved in protein strucutte
formation ? Write down their names.

[k.M – l
SECTION – C

¼y?kq mÙkjh; iz'u½

(Short Answer Type Questions)

22. ySECMk Qst thukse dh fo'ks"krk,¡ D;k gSa vkSj Dyksfuax ds fy, ;s D;ksa egÙoiw.kZ gSa \ 11 + 11 = 3
2 2

What are the characteristics of the Lambda Phage Genome, and why are these

important for cloning ?

2233 P. T. O.

( 14 ) 2233
23. ekl LisDVªksesVªh D;k gS \ ekl LisDVªksehVj dh :ijs[kk cukb,A 11 + 11 = 3
2 2
What is Mass Spectrometry ? Draw the outline of Mass Spectrometer.

vFkok
OR

NCBI dk iwjk uke fyf[k,A NCBI esa vk.kfod thofoKku ls lacaf/kr fdrus izdkj ds lalk/ku miyC/k
gSa \ 1+2=3

Write down the full form of NCBI. How many types resources regarding
molecular biology are available in the NCBI ?

24. thuksfeDl dks ifjHkkf"kr djsaA lajpukRed vkSj dk;kZRed thuksfeDl ds chp vUrj crkb,A 1+2=3

Define Genomics. Differentiate between Structural and Functional Genomics.

25. mRifjorZu p;u fof/k D;k gS \ bldk mi;ksx lw{etho lEo/kZ dks csgrj cukus ds fy, dSls fd;k tkrk
gS \ 11 + 11 = 3
2 2
What is Mutation Selection Methods ? How it is used to improve the microbial
culture ?

26. xksYMu jkbl D;k gS \ bls fodflr djus okys oSKkfudksa ds uke crkb,A vkt dh nqfu;k esa bldk egÙo
crkb,A 1+1+1=3

What is Golden Rice ? Name the scientists who developed it. Explain its
significance in today's word.

27. i'kq dksf'kdk lao/kZu esa rkieku] ih-,p vkSj vkWLeksySfyVh dk D;k egÙo gS \ 1+1+1=3

What is the importance of Temperature, pH and Osmolality in animal cell
culture ?
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( 15 ) 2233
vFkok

OR

lkseSfVd gkbfczM D;k gSa \ le>kb,A 3

What are Somatic Hybrids ? Explain.

[k.M – n

SECTION – D

¼nh?kZ mÙkjh; iz'u½

(Long Answer Type Questions)

28. cSDVhfj;k esa izfrca/k ,atkbe dk D;k dk;Z gS \ izfrca/k LFky vkerkSj ij iSfyaMªksfed D;ksa gksrs gSa \
feFkkbys'ku cSDVhfj;k ds Mh ,u , dks izfrca/k ,atkbe }kjk ikpu ls cpkus esa dSls enn djrk gS \
1+2+2=5
What is the function of restriction enzymes in bacteria ? Why are restriction
sites usually palindromic ? How does methylation help protect the bacterial DNA
from digestion by restriction enzymes ?

vFkok
OR
esVkthuksfeDl D;k gS \ lw{etho lEo/kZ esa u, v.kqvksa dks la'ysf"kr djus ds fy, lw{etho thu dh
igpku vkSj p;u djus ds fy, bldk mi;ksx dSls fd;k tkrk gS \ 1+4=5
What is Metagenomics ? How it is used to identify and select microbial genes to
synthesize novel molecules in microbial culture ?

2233 P. T. O.

( 16 ) 2233
29. izksVhu fQaxj fizafVax D;k gS \ izksVhu fQaxj fizafVax esa 'kkfey pj.kksa dks fyf[k,A blds pj.kksa dk ¶ykspkVZ
cukb,A

1 + 21 + 11 = 5
2 2
What is Protein Finger Printing ? Write down the steps involved in protein finger
printing. Draw the flowchart of its steps.

vFkok
OR

ewy dksf'kdk vkSj ikni Ård lao/kZu rduhdsa fyf[k,A izR;kjksi.k lao/kZu dks foLrkj ls le>kb,A

21 + 21 = 5
2 2
Write down the basic cell and plant tissue culture techniques. Explain explant
culture in detail.

30. ekbØks,js rduhd D;k gS \ blds fl)kUr vkSj izfØ;k dks fp= dh lgk;rk ls foLrkj ls le>kb,A
1+4=5
What is Microarray Technology ? Explain its principle and procedure in detail
with the help of diagram.
vFkok
OR
LVse lsy rduhd D;k gS \ bls fp= dh lgk;rk ls foLrkj ls le>kb,A 1+4=5
What is Stem Cell Technology ? Explain it in detail with the help of diagram.

S
2233