HBSE Class 12 Question Paper 2023 Biotechnology

CLASS : 12th (Sr. Secondary) Code No. 233
Series : SS/Annual-2023
Roll No.

tSo izkS|ksfxdh
BIOTECHNOLOGY
[ Hindi and English Medium ]
ACADEMIC/OPEN

(Only for Fresh/Re-appear/Improvement/Additional Candidates)

Time allowed : 2:30 hours ] [ Maximum Marks : 60
• Ñi;k tk¡p dj ysa fd bl iz'u&i= esa eqfnzr i`"B 8 rFkk iz'u 18 gSaA
Please make sure that the printed pages in this question paper are 8 in number and
it contains 18 questions.

• iz'u&i= esa nkfgus gkFk dh vksj fn;s x;s dksM uEcj dks Nk= mÙkj&iqfLrdk ds eq[;&i`"B ij fy[ksaA
The Code No. on the right side of the question paper should be written by the
candidate on the front page of the answer-book.

• Ñi;k iz'u dk mÙkj fy[kuk 'kq: djus ls igys] iz'u dk Øekad vo'; fy[ksaA
Before beginning to answer a question, its Serial Number must be written.

• mÙkj&iqfLrdk ds chp esa [kkyh iUuk@iUus u NksMsa+A
Don’t leave blank page/pages in your answer-book.

• mÙkj&iqfLrdk ds vfrfjDr dksbZ vU; 'khV ugha feysxhA vr% vko';drkuqlkj gh fy[ksa vkSj fy[kk mÙkj u
dkVsaA
Except answer-book, no extra sheet will be given. Write to the point and do not
strike the written answer.

233 P. T. O.

(2) 233
• ijh{kkFkhZ viuk jksy ua0 iz'u&i= ij vo'; fy[ksaA jksy ua0 ds vfrfjDr iz'u&i= ij vU; dqN Hkh u
fy[ksa vkSj oSdfYid iz'uksa ds mÙkjksa ij fdlh izdkj dk fu'kku u yxk,¡A
Candidates must write their Roll No. on the question paper. Except Roll No. do not
write anything on question paper and don't make any mark on answers of objective
type questions.

• d`i;k iz'uksa ds mÙkj nsus lss iwoZ ;g lqfuf'pr dj ysa fd iz'u&i= iw.kZ o lgh gS] ijh{kk ds mijkUr bl
lEcU/k esa dksbZ Hkh nkok Lohdkj ugha fd;k tk;sxkA
Before answering the questions, ensure that you have been supplied the correct and
complete question paper, no claim in this regard, will be entertained after
examination.

lkekU; funsZ'k %
(i) bl iz'u&i= esa dqy 18 iz'u gSa] ftUgsa pkj [k.Mksa % v] c]
c] l vkSj n esa ck¡Vk x;k gSA lHkh iz'u
vfuok;Z gSaA

(ii) [k.M & v ds iz'u la[;k 1 esa ckjg (i-xii) oLrqfu"B iz'u gSa] ftlesa iz'u (i) ls (vii) rd
cgqfodYih; iz'u gSaA izR;sd iz'u esa pkj fodYi fn;s x;s gSa] ftlesa ls lgh fodYi dk p;u
dhft,A iz'u (viii) ls (xii) rd izR;sd iz'u dk mÙkj ,d ;k nks okD;ksa esa nhft,A çR;sd ç'u 1
vad dk gSA
(iii) [k.M & c esa iz'u la[;k 2 ls 10 rd vfry?kq mÙkjh; iz'u gSaA çR;sd ç'u 2 vadksa dk gSA
(iv) [k.M & l esa ç'u la[;k 11 ls 15 rd y?kq mÙkjh; iz'u gSaA çR;sd ç'u 3 vadksa dk gSA
(v) [k.M & n esa ç'u la[;k 16 ls 18 rd nh?kZ mÙkjh; iz'u gSaA çR;sd ç'u 5 vadksa dk gSA
(vi) ç'u&i= esa lexz:i ls dksbZ fodYi ugha gS ysfdu [k.M & n ds nks iz'uksa (16 ,oa 17) esa
vkarfjd fodYi fn, x, gSaA ,sls iz'uksa esa ls vkidks fn;s x;s fodYiksa esa ls dsoy ,d gh iz'u djuk
gSA
General Instructions : –
(i) This question-paper consist of 18 questions, which are divided into four
Sections : A, B, C and D. All questions are compulsory.

233

(3) 233
(ii) Question No. 1 of Section – A has twelve (i-xii) objective type questions in
which questions from (i) to (vii) are multiple choice type questions. Each
question carries four options, from which choose correct option and the
answer of each question from (viii) to (xii) may be given in one or two
sentences. Each question carries 1 mark.
(iii) Question Nos. 2 to 10 of Section – B are very short answer type questions.
Each question carries 2 marks.
(iv) Question Nos. 11 to 15 of Section – C are short answer type questions. Each
question carries 3 marks.
(v) Question Nos. 16 to 18 of Section – D are long answer type questions. Each
question carries 5 marks.
(vi) There is no overall choice but in two questions (16 & 17) of Section – D
internal choice are given. You have to attempt only one of the given choice in
such questions.

[k.M – v
SECTION – A
¼oLrqfu"B iz'u½
(Objective Type Questions)

1. (i) fuEu esa ls dkSu&lk xk; ds nw/k dh rqyuk esa ekuo ds nw/k esa vf/kd gksrk gS \ 1

(A) izksVhu (B) ySDVkst
(C) dSfYl;e (D) dslhu
Which of the following is more in human milk as compared to cow's milk ?

(A) Protein (B) Lactose

(C) Calcium (D) Casein

233 P. T. O.

(4) 233
(ii) fdl m|ksx esa ,atkbe ,sYdkysl dk iz;ksx gksrk gS \ 1
(A) lkcqu (B) fMVtsZUV
(C) (A) vkSj (B) nksuksa (D) peM+k
In which industry enzyme alcalase is used ?
(A) Soap (B) Detergent
(C) Both (A) & (B) (D) Leather
(iii) ,d ysVj dksM 'G' fdl vehuks vEy ds fy, gS \ 1
(A) XyqVkfeu (B) XywVSfed vEy

(C) (A) vkSj (B) nksuksa (D) buesa ls dksbZ ugha
One letter code 'G' stands for which amino acid :
(A) Glutamine (B) Glutamic acid

(C) Both (A) & (B) (D) None of these

(iv) izksfivksfucSDVhfj;e 'ksjeuh gS % 1
(A) xzksFk gkWeksZu (B) ,y&ykblhu
(C) foVkfeu B12 (D) balqfyu
Propionibacterium Shermanii is :
(A) Growth hormone (B) L-lysine
(C) Vitamin B12 (D) Insulin
(v) dkWfIVl tSiksfudk dk mRikn gksrk gS % 1
(A) dksMhu (B) ccZjhu
(C) dki~lSflu (D) fDouhu
The product of coptis Japonica is :
(A) Codeine (B) Berberine
(C) Capsaicin (D) Quinine

233

(5) 233
(vi) eDdk esa [kjirokj fu;a=.k ds fy, dkSu&lk thu izfo"V fd;k tkrk gS \ 1

(A) BtCryIA(b) (B) BtCryIA(c)

(C) EPSP flUFkst (D) ukbfVªyst
Which gene is introduced for weed control in corn ?

(A) BtCryIA(b) (B) BtCryIA(c)

(C) EPSP synthase (D) Nitrilase

(vii) fuEu esa ls dkSu eqdyu }kjk foHkkftr gksrk gS \ 1
(A) cSDVhfj;k (B) ;hLV
(C) dod (D) buesa ls dksbZ ugha
Which of the following divide by budding ?
(A) Bacteria (B) Yeast
(C) Fungi (D) None of these

(viii) ,d Øk;ksfiztosZfVo dk uke fyf[k,A 1
Name one cryopreservative.

(ix) us'kuy ck;ksesfMdy fjlpZ QkmaMs'ku }kjk LFkkfir izksVhu baQkWesZ'ku òksr ds D;k dk;Z gSa \ 1
What is the function of protein information source established by National
Biomedical Research Foundation (NBRF) ?

(x) vkerkSj ij lqjf{kr ik, tkus okys lwphc) jksxk.kqvksa (GRAS) ds D;k xq.k gksrs gSa \ 1
What is the property of Generally Regarded as Safe (GRAS) listed microbes ?

(xi) IykfTeM D;k gS \ 1
What is plasmid ?

(xii) 'kCn thuksfeDl nsus okys oSKkfud dk uke fyf[k,A 1
Name the scientist who coined the term Genomics.

233 P. T. O.

(6) 233
[k.M – c
SECTION – B

¼vfry?kq mÙkjh; iz'u½
(Very Short Answer Type Questions)

2. vkWxsZuksQkWLQsV ds fofHkUu O;qRiUuksa ¼MsfjosfVOl½ ds uke fyf[k,A 2

Name various organophosphates derivatives.

3. ddATP dh jklk;fud lajpuk fyf[k,A 2

Write chemical structure of ddATP.

4. Vªk¡lQsD'ku rduhd ds ckjs esa fyf[k,A 2
Write about transfection technique.

5. ;wdSfj;ksfVd thUl dh dk;kZRed vfHkO;fDr ds fy, ;hLV dk O;kid :i ls mi;ksx D;ksa fd;k x;k gS \ 2
Why have yeast been used extensively for functional expression of Eukaryotic
genes ?

6. D;wjsVj fdls dgrs gSa \ 2
What is a curator ?

7. thukse lhDosUl VSXl (GSTs) ,oa thuksfed Mh,u, ds ckjs esa fyf[k,A 2

Write about genome sequence tags (GSTs) and genomic DNA.

8. dYpj esa ukWeZy dksf'kdkvksa ls dSalj dksf'kdk,¡ fdl izdkj fHkUu gSa \ 2

How is Cancer cells differ from normal cells in culture ?

233

(7) 233
9. ySfeukj ,;j ¶yks gqM ds dk;Z D;k gSa \ 2

What are the functions of Laminar air flow hood ?

10. ,d izfr:ih ¼fVfidy½ jDr dksf'kdk fuekZ.k ds fofHkUu pj.k D;k gSa \ 2

What are the various stages of a typical blood cell formation ?

[k.M – l
SECTION – C
¼y?kq mÙkjh; iz'u½
u½
(Short Answer Type Questions)

11. fjfLVªD'ku ekWfMfQds'ku flLVe ds nks dEiksusUV~l fyf[k,A buds mi;ksx D;k gSa \ 3
Write down two components of restriction modification system. What is their
use ?

12. fdu 'kh"kksZa }kjk ,ulhchvkbZ (NCBI) ls miyC/k lalk/kuksa dks oxhZÑr fd;k tkrk gS \ 3

By which heads, the resources available from the NCBI are classified ?

13. cká ekbØksfc;y mRiknksa ds vyxko esa varfuZfgr fofHkUu pj.kksa ds fy, lkekU; izokg 'khV Mªk dhft,A 3
Draw a general flow sheet for various steps involved in isolation of extracellular
microbial products.

14. ikS/kksa esa uj vuqoZjrk ¼ca/;rk½ fdl izdkj izkIr dh tk ldrh gS \ 3

How can male sterility be obtained in plants ?

15. EPO ,oa r-HuEPO ds mi;ksx fyf[k,A 3

Write down the use of EPO and r-HuEPO.

233 P. T. O.

(8) 233
[k.M – n
SECTION – D

¼nh?kZ mÙkjh; iz'u½
(Long Answer Type Questions)

16. fQaxj fizafVax esa fufgr fofHkUu pj.kksa dk o.kZu dhft,A 5

Describe various steps involved in finger printing.

vFkok
OR

izksVhu lsijsfVax ¼i`Fkd djus½ ds fy, vkblksbysfDVªd Qksdflax rduhd dk o.kZu dhft,A
Describe isoelectric focusing technique for separating proteins.

17. ekbØksfc;y dYpj ds xzksFk dkbusfVDl dk o.kZu dhft,A 5
Describe growth kinetics of microbial culture.

vFkok
OR

ekbØksfc;y dYpj esa iz;qDr fofHkUu midj.kksa ds ckjs esa fyf[k,A
Write about various equipments used in microbial culture.

18. ikniksa esa osDVjysl ¼osDVj jfgr½ thu VªkalQj dh fof/k;ksa dk o.kZu dhft,A 5
Describe vectorless gene transfer methods in plants.

S
233