ISC Class 12 Question Paper 2020 for Biotech

BIOTECHNOLOGY
PAPER 1
(THEORY)
(Maximum Marks: 70)
(Time allowed: Three hours)
(Candidates are allowed additional 15 minutes for only reading the paper.
They must NOT start writing during this time.)
Answer Question 1 (compulsory) from Part I and five questions from Part II.
The intended marks for questions or parts of questions are given in brackets [ ].
PART I (20 Marks)
Answer all questions.
Question 1
(a) Mention any one significant difference between each of the following: [5]
(i) Nucleotide and Nucleoside
(ii) Gene and Genome
(iii) Finite cell lines and Continuous cell lines
(iv) Primer and Primase
(v) Micronutrients and Macronutrients

(b) Answer the following questions: [5]
(i) What are Polylinker sites?
(ii) What is Subtilisin?
(iii) Name two types of phage vectors.
(iv) What is the role of Agrobacterium tumefaciens in genetic engineering?
(v) Why is DNA replication called semi-discontinuous?

(c) Write the full form of each of the following: [5]
(i) DDBJ
(ii) HEPA
(iii) RFLP
(iv) VNTR
(v) MS Medium

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(d) Explain briefly the following: [5]
(i) Reverse transcription
(ii) Electroporation
(iii) Biolistic
(iv) Synthetic seeds
(v) Flavr savr tomatoes

PART II (50 Marks)
Answer any five questions.
Question 2
(a) Describe the double helical structure of DNA. Mention two differences [4]
between RNA and DNA.
(b) List the role of the following in protein synthesis: [4]
(i) mRNA
(ii) rRNA
(iii) tRNA
(iv) Ribosomes
(c) Why are cDNA libraries preferred over genomic libraries? [2]

Question 3
(a) Differentiate between each of the following: [4]
(i) Blunt end and Sticky end
(ii) Hybrid and Cybrid
(b) Discuss the role of Biotechnology in making the following: [4]
(i) Humulin
(ii) Hepatitis B vaccine
(c) What is FBS? What is its role in animal cell culture? [2]

Question 4
(a) What is SCP? Describe the advantages and disadvantages of SCP. [4]
(b) Explain the role of any four enzymes in the process of DNA replication. [4]
(c) What is micropropagation? Write its use. [2]

Question 5
(a) Draw a neat and labelled diagram of a bioreactor. [4]
(b) How did Messelson and Stahl prove the semi conservative mode of [4]
replication?
(c) What is the use of haemocytometer? [2]
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Question 6
(a) Give four points of difference between inducible operon and repressible [4]
operon.
(b) Write short notes on the following: [4]
(i) Western blotting
(ii) Tissue engineering
(c) What is embryo rescue? [2]

Question 7
(a) Explain the role of the following in biotechnology: [4]
(i) Thermus aquaticus
(ii) Bacillus thuringiensis
(iii) Escherichia coli
(iv) CaMV
(b) Explain the methodology involved in the creation of the first mammalian clone, [4]
Dolly.
(c) What is Golden rice? Why is it considered to be nutritionally superior to the [2]
normal rice?

Question 8
(a) Enumerate the steps involved in the extraction and purification of DNA from [4]
bacterial cell.
(b) Write short notes on the following: [4]
(i) EMBL
(ii) SWISS-PROT
(c) Write the difference between defined media and differential media. [2]

Question 9
(a) What is somatic hybridization? Explain the steps involved in this technique with [4]
the help of an example.
(b) What is genomics? What are the basic criteria in selecting the organism for its [4]
genome sequencing? Write the names of any two types of DNA used for
sequencing.
(c) Write a short note on Taxonomy Browser. [2]

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