Karnataka PGCET 2017 Question Paper Biotechnology

POST GRADUATE COMMON ENTRANCE TEST-2017
DATE and TIME COURSE SUBJECT
ME/M.Tech/M.Arch/
01-07-2017 courses offered by BIO-TECHNOLOGY
2.30 p.m. to 4.30 p.m. VTU/UVCE/UBDTCE
MAXIMUM MARKS TOTAL DURATION MAXIMUM TIME FOR ANSWERING
100 150 Minutes 120 Minutes
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A- 1. 121146
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IMPORTANT INSTRUCTIONS TO CANDIDATES
1. This question booklet contains 75 (items) questions and each question will have one statement and four answers.
(Four different options / responses.)
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Marks Distribution
PART-1 : 50 QUESTIONS CARRY ONE MARK EACH (1 TO 50)
PART-2 : 25 QUESTIONS CARRY TWO MARKS EACH (51 TO 75)

BT-Al 1111111111111111111111

BIOTECHNOLOGY

PART — A

Each question carries one mark. (50 x 1 = 50)

1. The transfer of genetic material from 5. The catalytic activity of two different
one cell to another by a phage is called enzymes can be compared by the
(A) Transformation (A) pH of optimum value

(B) Conjugation (B) Molecular size of enzyme

(C) Transduction (C) Formulation of the product

(D) 'Hybridization (1)) km value

2. The key enzyme found in PCR that is 6. Pribnao box contribute to
Taq polymerase is (A) Protein synthesis
(A) Methanogen (B) ATP synthesis
(B) Acidophil (C) RNA synthesis
(C) Thermophile (D) none of above
(D) Halophile
7. "Gene-battery model" of Gene
3. Which of the following is an a amino regulation in eukaryotes is proposed
acid ? by

(A) Serine (A) Jacob and Monad

(B) Threonine (B) Britten and Davidson

(C) Valine (C) Beadle and Tatum

(D) Proline (D) Kornberg and Ochoa

4. Most common monomer of 8. Motation is
carbohydrate is (A) Change that is inherited
(A) Nucleotides (B) Change in parent not inherited
(B) Glucose (C) Plant growth controlling factor
(C) Amino acids (D) Change that affects the
(D) Maltose offsprings of F, generation

BT 3 A-1

9. Which of the following 13. The alignment method suitable for
organelle/molecule is related with finding out conserved patterns of DNA
genetic engineering ? or protein sequences is
(A) Multiple sequence alignment
(A) Mitochondrion
(B) Pairwise alignment
(B) Golgi Apparatus
(C) Global alignment
(C) Plasmid (D) Local alignment
(D) Lysosome
14. Which of the following is the first
10. The transgenic animals are those biological database ?
which have (A) Genbank

(A) Foreign DNA in some of the (B) DDRI
cells. (C) Atlas of protein sequence and
structure
(B) Foreign DNA in all of the cells.
(D) OMIM
(C) Foreign RNA in all of their cells.
(D) Foreign DNA and RNA in all of 15. The first successfully cloned animal
their cells was
(A) Monkey
11. The first hormone artificially produced (B) Gibbon
by culturing bacteria is (C) Sheep
(A) Insulin (D) Rabbit

(B) Adrenali n
16. The technique used in animal bio
(C) Thyroxine
technology for rapid multiplication
(D) Testosterone and production of animals with
desirable genotype is
(A) Protoplast fusion and embryo
12. Submission to Gen Bank are made
transfer
using
(B) Hybrid selection and embryo
(A) Bankit and Sequin transfer
(B) Bankit and Bankin (C) Invitro fertilization and embryo
(C) Sequin and Rankin transfer
(D) Protoplast fusion and hybrid
(D) Entrez selection

A-1 4 BT

17. The most common solidifying agent 22. itai-itai disease is due to
used in micro propagation is contamenation of metal in
(A) Agar water body.
(B) Dextran (A) Cadmium
(C) Mannan (B) Mercury
(D) Agarose
(C) Selenium
(D) Lead
18. Sudden and sharp rise in rate of
respiration of matured fruits is called -
23. Algal blooms occur due to
(A) clinostat
Eutrophication of lakes & ponds. This
(B) climactrie rise
can be attributed to enr•icchment of
(C) circumvallation vortex
(A) Phosphates and carbonates
(D) red drop emerson effect
(B) Phosphates and nitrates
19. Richest source of vitamin A is - (C) Carbonates & bicarbonates
(A) Knol-khol (D) Sulphates, carbonates and
(B) Tomato phosphates
(C) Mango
(D) Capsicum 24. Selection of hybridoma for production
of monoclonal antibody is done by
20. Emblica officenale is richest source of (A) HGPRT
(A) Iron (B) HAT
(B) Cobalt rich vitamin (C) PEG
(C) Ascorbic acid
(D) Tween 20 & PEG
(D) Lysergic acid

25. Allergic contact dermatitis is
21. Increase in level of biodegradation
through manipulation of genes is type of hypersensitivity
called as - reaction.
(A) Biostimulation (A) Type I
(B) Biomagnification (B) Type II
(C) Bioaccumulation (C) Type III
(D) Bioaugmentation (D) Type IV

BT 5 A-1

26. Xenografts are rejected rapidly due to 30. The first completed genome
(A) rejection by IgM or cell sequencing project is of
mediated rejection. (A) E. coli
(B) host graft responses (B) Haemophilus influenzae
(C) lymphocyte attack on graft (C) (I) X 174
recipient Drosophila melanogastcr
(D)
(D) T-cell inactivation

31. The best method to protect genetic
27. Antiviral proteins, that regulate
resources is
immune response as cytokines arc
(A) Gene library
called as
(A) Interleukin (B) Cloning of plants
(B) Interferon (C) Cryopreservation
(C) Chemokines (D) Multiplication
(D) Monokines
32. The term genomics is coined by
28. CDR's (Complementarity determining (A) Thomas Cech
Regions) are — (B) T.H. Morgan
Antigen binding fragment of Ig (C) Thomas Roder
generated by proteolysis.
(D) Craig Venter
Crystallisable fragment of Ig
generated by proteolysis,
medietes phagocytosis and 33. Which amino acid is phosphorylated
triggers inflammation. in bacterial proteins
Hypervariable loop in (A) Arginine
immunoglobulin polypeptide (B) Cysteine
forms antigen bending site. (C) Lysine
Materials that elicits antibody (D) Histidine
production.

34. Which of the following is NOT a
29. Immunoglobulin abundantly found in
common feature of reterovirus ?
milk, sweat, tear and colostrum is
(A) They are enveloped
(A) IgG
(B) IgE (B) Their RNA is spliced
(C) IgA (C) They contain ETR's
(D) IgM (D) They integrate into host DNA

A-1 6 BT

35. Parkinson's disease is characterised by 39. The first protein to have its primary
cell death in the structure determined was
(A) spinal cord (A) Urease
(B) substantia nigra (B) Insulin
(C) Frontal cortex (C) Glucagon
(D) Motor cortex (D) Histone

36. Which phytohonnone induces seed
40. In which of the disorders, blood has
dormancy ?
defective hemoglobin ?
(A) Gibberellin
(A) hemophila
(B) Abscisic Acid
(B) hematuria
(C) Auxin
(C) hematoma
(D) Ethylene
(D) sickle cell anaemia
37. The released energy obtained by
oxidation of glucose is stored as 41. When cells respond to an extracellular
(A) a concentration gradient across a signal,, they most often convert the
membrane information from one form to another.
(B) ADD This process is called :
(C) ATP (A) signal transformation
(D) NAD- (B) signal transduction
(C) signal interference
38. A kinase is an enzyme that (D) signal amplification
(A) removes phosphate groups of
substrates
42. Albinism is a congenital human
(B) Uses ATP to add a phosphate disorder resulting from lack of enzyme
group to the substrate
(A) Catalase
(C) Uses NADH to change oxidation
(B) Fructokinase
state of the substrate
(D) (C) Tyrosinase
Removes water from double
bond (D) Xanthine oxidase

BT 7 A-1

43. Small charged molecules, often 47. The gene not expressing any protein is
biogenie amines function as known as

(A) Hormones (A) Epistatic gene

Neurotransmitters. (B) Hypostatic gene
(B)
(C) Pseudo gene
(C) Harmones and Neurotransmitters
(D) Split gene
(D) Transmitter

48. An open system in which the growth
44. The terminator codons are rate is maintained by the removal and
% A If A /- I' r"
addition of media at such a rate as to
(A )
maintain a constant call density is
(B) AUG. LAG. UGA called

(C) UAC, AUG, UAG ( A ) manostat

(D) AUG, ACG, GAG (B) chemostat
(C) tubidostat

45. Transcription takes place in (D) culturostat

(A) Matrix
49. The pairing of homologous
(B) Nucleus chromosomes
(C) Cytosol (A) Tetrad's

(D) Cytoplasm (B) Crossing over
(C) Synapsis
(D) Terminalization
46. The unwinding of DNA duplex is
performed by an enzyme called
50. Enzyme papain is used with success to
(A) Gyrase
(A) Increase meat production
(B) Lactase
(B) Ripen papaya fruit
(C) Maltase
(C') Leaven bread
(D) Ligase (D) Tenderize meat

A-1 8 BT

PART — B
Each question carries two marks. (25 x 2 = 50)

51. The ability of bacteria to change their 56. One of the following is a transgenic
morphological form frequently is organism :
termed as :
(A) Flavr Savr tomato and BT cotton
(A) Lysogeny
(B) Pleomorphism (B) Dolly sheep and BT cotton
(C) Alteromorphism (C) Holly sheep and BT cotton
(D) Polymorphism (D) Holly sheep and Flavr savr
tomato
52. Bacterial chromosome is
(A) Single stranded and linear
(B) Single stranded and circular 57. Fisher and Krebs got Nobel Prize in
1992 for their discovery of —
(C) Double stranded and linear
(D) Double stranded and circular (A) Organ and Cell Transplantation
(B) Reversible protein phosphorylation
53. Positively charged basic amino acids are as biological regulatory mechanism
(A) Lysine and glutamine (C) Single ion channels
(B) Glutamine and arginine
(D) All of the above
(C) Lysine and aspargine
(D) Lysine and arginine
58. A gene that specifies the amino acid
54. All of them are monosaccharides sequence of a polypeptide chain
except termed as
(A) Glucose (A) Structural gene
(B) Fructose
(B) Regulator gene
(C) Maltose
(C) Operator gene
(D) Galactose
(D) Split gene
55. The Ti-plasmid is often used for
making Transgenic plants. This 59. Which of the following is a sequence
plasmid is found in — alignment tool ?
(A) Azo bacter
(A) BLAST
(B) Riz obium roots of Leguminous
plants (B) PRINT
(C) Agrobacteri um (C) PROSITE
(D) Yeast as in vim plasmid (D) PIR

BT 9 A-1

60. All the following are protein sequence 64. Genome wise gene expression analysis
databases except is performed using
(A) P1R (A) DNA microarrays
(B) PSD (B) Northern blotting
(C) SWISS PROT Real time PCR
(C)
(D) EMBL
(D) RT-PCR

61. In callus culture
65. To determine 'variation in wing length
(A) Increasing level of Cytokinin to
a callus induces shoot formation of butterfly from five different places.
and increasing le\,-cl of auxin Which would be the best statistical
promote root formation. test ?
(B) Increasing level of auxin to a (A) F Test
callus induces shoot formation
and increasing level of cytokinin (B) Student T-test
promote root formation (C) Regression Analysis
(C) Auxins and cytokinins are not (D) Chi-square test
required
(D) Only auxin is required for root
and shoot formation. 66. C language developed at
(A) AT & T's Bell laboratories of
62. Poise is unit of USA in 1972
(A) Surface tension (B) AT & T's Bell laboratories of
(B) Viscosity USA in 1970
(C) Intensity of fire (C) Sun Microsystems in 1973
(D) Angular momentum (D) Cambridge University in 1977

63. bioreactor is used for 67. Travelling salesman problem is an
temperature dependent formation of
example of
products.
(A) Tower hioreactor (A) Dynamic Algorithm

(B) Bubble column hioreactor (B) Greedy Algorithm
(C) Stirred tank bioreactor (C) Recursive Approach
(D) Two stage airlift hioreactor (D) Divide and Conquer

A-1 10 BT

68. If a father is blood group 'AB' and 72. Which enzyme causes releases of
mother is blood group '0', what blood pyrophosphate from ATP ?
group their children will he never (A) Glycogen Phosphorylases
have ? (B) RNA polymerase
(A) AB
(C) ATPase
(B) 0
(D) Aspartate transcarbamylase
(C) A&B
(D) AB & 0
73. Chloroform is more miscible with
water than carbon tetrachloride
69. Amino acid derivative functioning as because chloroform has a
chemical messenger in communication
(A) lower dipole moment than
between cells carbon tetrachloride
(A) Dopamine
(B) lower dielectric constant than
(B) Histamine carbon tetrachloride
(C) Thymine (C) higher dipole moment than
(D) GABA carbon tetrachloride
(D) higher order of symmetry than
70. The Enzyme telomerase has carbon tetrachloride
(A) Polymerase activity
(B) Ligase activity 74. Which compound cannot cross the
(C) Reverse transcriptase activity inner membrane of mitochondria ?
(D) Hydrolases activity (A) ATP
(B) Pyruvate
71. Why klenow DNA polyrnerase cannot (C) Acetyl CoA
be used in nick translation ? (D) Pyrophosphate
(A) Because it has 5' --> 3',
exonuclease activity
75. Which of the following common drugs
(B) Because it has 3' —>
is not a specific enzyme inhibitor ?
exonuclease activity
(A) Sulfonil amide
(C) Because it does not have 5' —> 3',
(B) Iodine
exonuclease activity
(C) Methotrexate
(D) Because it does not have 5' --> 3',
endonuclease activity (D) Azeduvidine

BT 11 A-1

MI • Mal

I

A-1 12 BT