JKBOSE 12th Class Model Paper Biotechnology

Biotechnology
Model paper 12 111 class

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All questions arc compulsory
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Q.No. l :- Given the fo llowing DN/\ strand : 5'-TCT A/\TGG/\CiC'l. wr ite d111,1 n th e
complementary strand. ----------------------. Indi cate the directi ons by properly labeling the 5'
end.

Q. N0.2:- Which gL'nc transfer method is most w 111111only used in plants'.)

Q.N0.3:- What is the IUPAC code for 'G' or 'C'?

Q. N0.4:- Write one therapeutic app licati on of monoclonal antibody.

Q. N0.5:- Rapid 111ul1iplica1ion of plants by tissue culture techniques is referred to as- ----------

7 , .:: morks

Q.N0.6:- A DNA molec ule is formed by linking the ------------------------or one nucleotide to
the--------------------------------of the neighboring nucl eotide.

Q.:\'0. 7: - Which tran sfo rmat ion nK·thod(s) u~cs (A) mechanical means or introduci ng l)i\ ,\
into cells') (H) Riological im:ans'.)

Q.N0.8:-. What is the unique feature of the structure of DNA or protein that causes tlw
macromolecules to mi grate when an electrical field is applied to the gel9

Q.N0.9:-.An E. coli ce ll produces at least 2000 different proteins. One of these 1s ou r
enzyme or interest produced at a lei cl or 3000 molecules per ce ll under opt im um conditions .
lf'we have to puril)' lg oi' thi s intrn-ccllula1· cn1y ml'. estimate ho1, nrnn: cl'ib urbm:tcria 11 il l
be required theoreticall y0 It is gill'll that the mo lecu lar weight or th e en ;: 11ll' or i11te1·l·st is
1,00,000.

Q.NO 10:- Can yo u suggest one reaso n why the seq uences or bacterial replication origins are
A-T rich?

Q. NO 11 :- In ½hat wa) (ill Iden ricL· is di l'i'e1·L·Jll from the nll rn,:il m·,,·1

Q.NO 12:- Write two important lcaturcs or cultured animal eel ts·J

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~_:::;_:=:.:.::::.::~"- / '

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Short Answer Question s 12 x 3marks

(). NO. 1.,: -.< ii , en the l<il lc1"ing DN/\ sequem:e. S' /\C'(iTGCTTC(i 3' Codin l-!, strand

3' T(iC'/\C(i( i/\(i(' 5'

(a) Which strand is the sense strand'? /\ntisense strand?

(h) What is the sequence of mRNA after transcripti on?

l e) Whal is th e sequ cn<.:e nfthe anti sensc RN/\'?

<). \ 0 1-l :- (i i, ,· e, :1111plcs Pl. pairs <l f amino acid s \ha\ form (/\) ekctrostatic int eractions.

(Bl hydrogen b\lndin g. (C) hydrophobic interactions.

Q.NO 15:- Why is it that genomic libraries are used for isolating genes in bacteria? Why

:ire cD\ ,\ lihrarie~ nu t used fo r prokaryotes?

().\0 I (1:- I . co li is ,\ rud shaped bacteria about 2 µm long and I ~un in diameter. The
a, cragc drnsit ~ of a cell is 1 .28 g/ ml. Approx imatel y 13.5% of the wet weight of E. coli is
solu ble protein . Estimate the number of molecules of a particular enzyme per cell if the
enzyme has a molecular we ight of I 00,000 and represents 0.1 % of the total soluble protein.

Q.\O 17:- h 1r hybricli1:1tio11. the DN1\ bo un d 10 the membrane is first denatured. What is
de n,1\ur;i\io n·.1 11<1,, do, tiu denature DN i\ on a membrane'?

Q.\O 18:- l3ric!ly explain how the serine residue in some enzy mes can become acidic
(reacti ve).Also suggest how yo u can confirm that a serine residue is involved in the catalysis.

Q. NO 19 :- Frozen cel ls shoul d be th awed as rapidl y as possible durin g their revival. Why?
\~·hat i:. tile role ti l' 1)\~SO in cryoprescrvati on')

Q.\O 20:- Wh: is ;\gn1 bac\eriu 111 described as 'natural genetic enginee r of plants''?

Q.NO 21 :- I low arc monoclonal antibodi es different fro m polyclonal antibodies?

Q. NO 22 :- Whal is the ro le of serum fo r culturing animal cells?

Q. NO 2J: - ls the concept ol' hy bridi zati on applicable to Western blot? Explain your answer?

Q.\O 24:- Whal ,,ere the surprises rcYeakd from genome sequencin g?

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Q .No.25:- Defin e·· pnmai
· .Y strncturc,
. .. secondary structure, terti.ary structure, and qu atern ary
stnict~irc._of a protein . What arc the major fo rces in vo lved in the form ati on of eac h stru ctu ra l
orga n1 zat1 on?

Or

What are the unique fea tures and properti es ora cosmid that make it desira ble as a cloning
vector?

Q .No.26:- What are the different types of molecules on which sequence data is ohtaincd and.

depos ited in the database•)

Or

Describe the methods used for the measurement and quantitati ve evaluation of micro bial
growth0

Q. No.27:- How are plant cells convened to protoplasts? Wh y are protoplasts mo re :1111enablc
to produce somati c cell hybrids and cy brids?

Or

What are stem cells') Describe the ,lpplication of embryo ni c stem cell technolog, '.1