NCERT Solutions for Class 12 Biology Chapter 9 Biotechnology : Principles and Processes

NCERT
SOLUTIONS
CLASS - 12th

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Class : 12th
Subject : Biology
Chapter : 11
Chapter Name : Biotechnology : Principles and Processes

Q1 Can you list 10 recombinant proteins which are used in medical Practice? Find out where they
are used as therapeutics (use the internet).

Q2 Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate
DNA on which it acts, the site at which it cuts DNA and the product it produces.

Q3 From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in
molecular size? How did you know?

Q4 What would be the molar concentration of human DNA in a human cell? Consult your teacher.

Q5 Do eukaryotic cells have restriction endonucleases? Justify your answer.

Q6 Besides better aeration and mixing properties, what other advantages do stirred tank
bioreactors have over shake asks?

Q7 Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to
create a palindromic sequence by following base-pair rules

Q8 Can you recall meiosis and indicate at what stage a recombinant DNA is made?

Q9 Can you think and answer how a reporter enzyme can be used to monitor transformation of
host cells by foreign DNA in addition to a selectable marker?

Q10 Describe brie y the following:
(a) Origin of replication
(b) Bioreactors
(c) Downstream processing

Q11 Explain brie y
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase

Q12 Discuss with your teacher and nd out how to distinguish between
(a) Plasmid DNA and Chromosomal DNA
(b) RNA and DNA

(c) Exonuclease and Endonuclease