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3312 (NS)
!3312NSMicrobiology! £vÄ Gs
Register Number
PART - III
~sq°›¯À / MICROBIOLOGY
( uªÌ ©ØÖ® B[Q» ÁÈ / Tamil & English Version)
Põ» AÍÄ : 3.00 ©o ÷|µ® ] [ ö©õzu ©v¨ö£sPÒ : 70
Time Allowed : 3.00 Hours ] [ Maximum Marks : 70
AÔÄøµPÒ : (1) AøÚzx ÂÚõUPЮ \›¯õP £vÁõQ EÒÍuõ GߣuøÚ
\›£õºzxU öPõÒÍÄ®. Aa_¨£vÂÀ SøÓ°¸¨¤ß AøÓU
PsPõo¨£õÍ›h® EhÚi¯õPz öu›ÂUPÄ®.
(2) }»® AÀ»x P¸¨¦ ø©°øÚ ©mk÷© GÊxÁuØS®,
AiU÷PõikÁuØS® £¯ß£kzu ÷Ásk®. £h[PÒ ÁøµÁuØS
ö£ß]À £¯ß£kzuÄ®.
(3) ÷uøÁ¯õÚ Ch[PÎÀ £h[PÒ ÁøµP.
Instructions : (1) Check the question paper for fairness of printing. If there is any lack of
fairness, inform the Hall Supervisor immediately.
(2) Use Blue or Black ink to write and underline and pencil to draw diagrams.
(3) Draw diagrams wherever necessary.
£Sv & I / PART - I
SÔ¨¦ : (i) AøÚzx ÂÚõUPÐUS® Âøh¯ÎUPÄ®. 15x1=15
(ii) öPõkUP¨£mkÒÍ ©õØÖ ÂøhPÎÀ ªPÄ® Hئøh¯
Âøhø¯z ÷uº¢öukzxU SÔ±mkhß Âøh°øÚ²® ÷\ºzx
GÊuÄ®.
Note : (i) Answer all the questions.
(ii) Choose the most appropriate answer from the given four alternatives and
write the option code and the corresponding answer.
[ v¸¨¦P / Turn over
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3312 (NS) 2
1. EHF Gß-£-x __________ .
(A) Ebola hemolytic fever (B) Ebola haemorrhagic fever
(C) Ebola heart fever (D) Ebola human fever
EHF stands for __________.
(a) Ebola hemolytic fever (b) Ebola haemorrhagic fever
(c) Ebola heart fever (d) Ebola human fever
2. ¤ß-Á-¸-Á-Ú-ÁØ-ÔÀ Gx ì÷£õ-÷µõ-]-hÀ AÀ» ?
(A) BÀ-P-íõÀ (B) SÐm-h-µõÀ-i-øí-k
(C) Gz-v-½ß BU-øé-k (D) £õº-©õÀ-i-øí-k
All of the following are sporicidal except :
(a) Alcohol (b) Glutaraldehyde
(c) Ethylene Oxide (d) Formaldehyde
3. QøÍ-Põ-øÍ-]-êß ÂøÚ¨-£k ö£õ-¸Ò Gß-£x :
(A) SÐU-÷Põì-6-£õì-÷£m (B) ø£¸-ÂU Aª-»®
(C) ]m-›U Aª-»® (D) SÐU-÷Põì
The reactant in glycolysis is __________.
(a) Glucose-6-phosphate (b) Pyruvic acid
(c) Citric acid (d) Glucose
4. ~s-q-°-›-PÒ Á͵ HØÓ pH ©v¨-¦ __________.
(A) 9.2 (B) 4.0 (C) 7.0 (D) 3.4
The microorganisms grow best at pH value around __________.
(a) 9.2 (b) 4.0 (c) 7.0 (d) 3.4
5. ]Á¨¦ vµõmø\ 12 •uÀ 36 ©o ÷|µ® ö|õ-vU-P¨-£-k® ÷£õ-x __________
u¯õ-›U-P¨-£-k-Q-Óx.
(A) Á¯-»m J°ß (B) ]Á¨¦ J°ß
(C) -CÍg-]-Á¨¦ J°ß (D) öÁÒøÍ J°ß
__________ is obtained from red grapes in which fermentation lasts for only
12 to 36 hours.
(a) Violet wine (b) Red wine
(c) Pink wine (d) White wine
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3 3312 (NS)
6. ¤ß-Á¸
- Á
- Ú
- Á
- Ø-ÔÀ G¢u •øÓ i.÷£-¼h
- ® Ps-hÔ
- ¯ £¯ß-£k
- z-u¨-£k
- Q
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(A) C®-³-÷Úõ ¦÷Íõ-µ-éßì \õ-¯-÷©Ø-ÓÀ
(B) ]À-Áº Em-¦-Szx •øÓ
(C) hõºU ¥Àm ~s-÷nõU-Q-°-¯À
(D) ÷©Ø-T-Ô¯ AøÚz-x®
Which of the following methods can be used to demonstrate T. Pallidum ?
(a) Immuno fluorescence staining
(b) Silver impregnation method
(c) Dark ground microscopy
(d) All the above
7. ½è-÷©-Û-¯õ E°-› ©Û-uÛØS __________ BÀ Phz-u¨-£-k-Q-Ó-x.
(A) öµk-Âm §a-] (B) \õsm §a-]
(C) -öhìi §a-] (D) öPõ-_
Leishmania organisms are transmitted to human by __________.
(a) Reduviid bug (b) Sand flies
(c) Tsetse flies (d) Mosquitoes
8. ©¸z-xÁ ø©U-Põ-»-â-°ß u¢-øu __________ BÁõº.
(A) Bs-hß i £õ-› (B) £õìa-\º
(C) ÷µ©sm áõU-SöÁì \õ¨-µõm (D) µõ-£ºm ÷Põa
__________ is the ‘Father of medical mycology’.
(a) Anton de Bary (b) Pasteur
(c) Raymond Jacques Sabouraud (d) Robert Koch
9. ø\m-÷hõ-ö©-Põ-÷»õ- øÁ-µì-PÒ __________ GßÖ AøÇU-P¨-£-k-Q-Ó-x.
(A) G¨ì-iß & £õº øÁµì (B) EªÌ-}º _µ¨¤ øÁµì
(C) ö|g-\U Pøn-¯ _µ¨¤ øÁµì (D) |õÍ-ªÀ-»õ _µ¨¤ øÁµì
Cytomegalo viruses are also called as _________.
(a) Epstein - Barr virus (b) Salivary gland virus
(c) Thymus gland virus (d) Endocrine gland virus
[ v¸¨¦P / Turn over
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3312 (NS) 4
10. öPõ_ ‰»® £µ-Ä® ©Ø-Ö® Eso ‰»® £µ-Ä® øÁµì-P-ÐUS Euõ-µ-n®
u¸-P ?
(A) öí¨-£-øh-iì øÁµì (B) öh[S øÁµì
(C) L¤Íõ øÁµì (D) ]U-Pß Sß-¯õ øÁµì
An example for mosquito-borne and tick-borne virus :
(a) Hepatitis virus (b) Dengue virus
(c) Flavi virus (d) Chikungunya virus
11. ÁøP II ªøP Tº EnºÄ ÂøÚ-P-ÎÀ __________ Dk-£-k-Q-Ó-x.
(A) IgE Bß-i-£õ-i (B) IgG Bß-i-£õ-i
(C) IgG ©Ø-Ö® IgM Bß-i-£õ-i (D) IgM Bß-i-£õ-i
In type II hypersensitivity reaction __________ is involved.
(a) IgE antibody (b) IgG antibody
(c) IgG and IgM antibodies (d) IgM antibody
12. i.Gß.-H __________ BP¨ £i-ö¯-kU-P¨-£-k-Q-Ó-x.
(A) hn & Bº.-Gß.-H. (B) m & Bº.-Gß.-H.
(C) t & Bº.-Gß.-H. (D) s & Bº.-Gß.-H.
DNA is transcribed into _________.
(a) hnRNA (b) mRNA
(c) tRNA (d) sRNA
13. G»Um-µõß ~s-÷nõU-Qø¯ __________ ©Ø-Ö® __________ •uß •u-¼À
Ps-k-¤-iz-u-Úº.
(A) Hº-Úìm µì-Põ ©Ø-Ö® ÷©Uì |õÀ
(B) ÷Põa ©Ø-Ö® ¼ì-hº
(C) £õìa-\º ©Ø-Ö® ¤öÍ-ª[
(D) öéº-ÛU ©Ø-Ö® íüU
Electron microscope was first invented by __________ and __________.
(a) Ernst Ruska and Max Knoll
(b) Koch and Lister
(c) Pasteur and Flemming
(d) Zernike and Hook
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5 3312 (NS)
14. ø|^-›-¯õ ö©Ûß-âi-iì __________ BS®.
(A) Qµõ® £õ-]m-iÆ i¨-÷Íõ-PõU-øP
(B) Qµõ® £õ-]m-iÆ PõU-øP
(C) Qµõ® ö|Pm-iÆ Sa-]
(D) Qµõ® ö|Pm-iÆ i¨-÷Íõ-PõU-øP
Neisseria meningitidis is __________.
(a) Gram positive diplococci
(b) Gram positive cocci
(c) Gram negative rod
(d) Gram negative diplococci
15. Gs- h - « - £ õ îì- ÷ hõ- ø »- i U- P õ- Â ß __________ ©Û- u ö£¸[- S - h - ¼ ß
÷Põ-øÇ¨-£-h-»® ©Ø-Ö® ÷Põ-øÇ¨-£-h-»U RÌ AkU-S-P-ÎÀ Áõ-Ê®.
(A) •ß-]ìm (B) ]ìm
(C) m÷µõ-÷£õ-\õ´-k (D) ÷©÷»-²ÒÍ AøÚz-x®
__________ of Entamoeba histolytica live in the mucous and submucous layers of
human large intestine.
(a) Precyst (b) Cyst
(c) Trophozoite (d) All the above
£Sv & II / PART - II
SÔ¨¦ : GøÁ÷¯Ý® BÖ ÂÚõUPÐUS Âøh¯ÎUPÄ®. ÂÚõ Gs 24 &US
Pmhõ¯©õP Âøh¯ÎUPÄ®. 6x2=12
Note : Answer any six Questions. Question No. 24 is compulsory.
16. rDNA öuõ-ÈÀ ~m-£® Gß-ÓõÀ GßÚ ?
What is rDNA technology ?
17. Bß-i-£-¯õ-iU & Áøµ-¯-ÖU-P-Ä®.
Define antibiotic.
18. ]m-›U Aª-»z-vß £¯ß-PÒ Cµs-iøÚ GÊ-x-P.
Write two uses of citric acid.
[ v¸¨¦P / Turn over
Page 7
3312 (NS) 6
19. Põ-\-÷|õ´ (TB) Gvº ©¸¢-x-PøÍ £m-i-¯-¼-k-P.
List out the antituberculosis drugs.
20. øh©õº-¤U §g-ø\-PÒ Gß-ÓõÀ GßÚ ?
What are Dimorphic fungi ?
21. Bß-i-öáß & Bß-i-£õi Gvº-Â-øÚ-PÒ Gß-ÓõÀ GßÚ ?
What are Antigen - Antibody reactions ?
22. ÷£ìPõßm-µìm ~s-÷nõU-Q-°ß £¯ß-PÒ Cµs-iøÚ GÊ-x-P.
Write any two applications of Phase contrast microscope.
23. ©x-µõ ø©U-÷Põ-^ì Gß-ÓõÀ GßÚ ?
What is Madura mycosis ?
24. ¸¢- ÷ uõ®- ¤ US Gv- µ õÚ Jm- k - Â ß {µõ- P - › ¨¦ {PÌÄ G¢- { - ø »- P - Î À
HØ£-k® ?
When does the graft versus host rejection occur ?
£Sv & III / PART - III
SÔ¨¦ : GøÁ÷¯Ý® BÖ ÂÚõUPÐUS Âøh¯ÎUPÄ®. ÂÚõ Gs 33 &US
Pmhõ¯©õP Âøh¯ÎUPÄ®. 6x3=18
Note : Answer any six Questions. Question No. 33 is compulsory.
25. DNA Á›-ø\¨-£-kz-x-uÀ •øÓ-P-øÍ £ØÔ GÊ-x-P.
Write the DNA sequencing methods.
26. EnÄ £u¨-£-kz-x-u-¼ß |ßø© wø©-PøÍ GÊ-x-P.
Write the advantages and disadvantages of food preservation methods.
27. ö|õ- v z- u À ÷©À ÷|õUS •øÓU- S ®, ö|õ- v z- u À RÌ ÷|õUS •øÓU- S ®
Cøh-÷¯-²ÒÍ ÷ÁÖ-£õ-k-PøÍ Am-h-Á-øn¨-£-kz-x-P.
Tabulate the difference between upstream and downstream in fermentation
process.
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7 3312 (NS)
28. HBV &°ß Aø©¨ø£ £h® Áøµ¢x £õ-P[-PøÍ SÔU-P-Ä®.
Draw and label the structure of HBV.
29. PCR &ß £i-{-ø»-PøÍ GÊx-P.
Write down the steps involved in PCR.
30. öẮ SÇõ´ ÷\õ-uøÚ & Áøµ-¯-ÖU-P-Ä®.
Define Germtube test.
31. Aª-¤-¯õ-]ì uk¨¦ ©Ø-Ö® Pm-k¨-£õk •øÓ £ØÔ ‰ß-ÔøÚ GÊ-x-P.
Write three methods in prevention and control of Amoebiasis.
32. ìøh-ø£-÷»õ-PõUøP ÷|õ´ öuõØ-Ö-PøÍ GÆ-Áõ-ÓõP ÁøP¨-£-kz-u-»õ® ?
How are Staphylococcal infections classified ?
33. ö|õv JÊ[-S¨-£-kz-x-u-¼À uk¨-£õ-Ûß ÁøP-P-øÍ ÁÈ-•-øÓ Áøµ-£-hz-xhß
GÊx-P.
Draw the flow chart, write the types of inhibition in enzyme regulation.
£Sv & IV / PART - IV
SÔ¨¦ : AøÚzx ÂÚõUPÐUS® Âøh¯ÎUPÄ®. 5x5=25
Note : Answer All the Questions.
34. (A) ¦Ð-µ-\ßm ~s-÷nõU-Q-°À £¯ß-£-kz-u¨-£-k® £À-÷ÁÖ ÁøP-¯õÚ
Ái-Pm-i-PÒ ¯õøÁ ?
AÀ»x
(B) ¨-›-÷¯õ Põ-»-µõ-Âß ÷|õ´ ÷uõØ-Ó® £ØÔ ÂÍU-S-P.
(a) What are the different types of filters used in fluorescence microscope ?
OR
(b) Explain the pathogenesis of Vibrio cholerae.
[ v¸¨¦P / Turn over
Page 9
3312 (NS) 8
35. (A) QøÍ-Põ-ø»-]ì & ÂÁ-›U-P-Ä®.
AÀ»x
(B) ©Û-u-øÚ öuõØ-Ö® 40 cm }Í® ÁÍ-¸® ö£›-¯ E¸-øÍ-¦-Ê-Âß ÁõÌUøP
_ÇØ]ø¯ ÂÁ-›U-P-Ä®.
(a) Describe Glycolysis.
OR
(b) Describe the life-cycle of large roundworm which grows to a length of upto
40 cm that infects humans.
36. (A) £õU-j-›-]-hÀ ©Ø-Ö® £õU-j-›-÷¯õì-hõ-iU-PõÚ ÷ÁÖ-£õ-k-PÒ I¢-vøÚ
GÊ-xP.
AÀ»x
(B) SCP EØ-£z-v-°À EÒÍ-h[-Q¯ £i-{-ø»-PøÍ GÊx-P.
(a) Write any five differences between Bactericidal and Bacteriostatic.
OR
(b) Write the steps involved in SCP production.
37. (A) En-Ä |g-\õ-uÀ ÁøP¨-£-kz-x-P.
AÀ»x
(B) ÷µ¥ì øÁµì Aø©¨ø£ ÂÁ-›U-P-Ä®.
(a) Classify the types of food-poisoning.
OR
(b) Describe the structure of Rabies virus.
38. (A) Gø»-\õ ÷\õ-u-øÚ-°ß Ai¨-£-øhz uz-x-Ázøu £hz-x-hß ÂÍU-S-P.
AÀ»x
(B) Kº E¯º-{ø» Phz-v-°ß (ideal vector) £s-¦-PøÍ GÊ-x-P.
(a) Explain the principle of ELISA with neat sketch.
OR
(b) Write the characteristics of an ideal vector.
- oOo-