JAM 2022 Question Paper Biotechnology (BT)

JAM 2022 BIOTECHNOLOGY - BT

Section A: Q.1 – Q.10 Carry ONE mark each.

Q.1 Which of the following is involved in innate immune response in higher
mammals?

(A) T cell antigen receptor

(B) B cell antigen receptor

(C) Toll-like receptor

(D) Major histocompatibility complex-II molecule

Q.2 Which among the following belongs to the family “Retroviridae”?

(A) Human Immunodeficiency virus

(B) Ebola virus

(C) Dengue virus

(D) Influenza virus

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JAM 2022 BIOTECHNOLOGY - BT

Q.3 Which of the following is a glycolipid?

(A) Cerebroside

(B) Phosphatidylcholine

(C) Phosphatidylserine

(D) Cardiolipin

Q.4 Which of the following bacterial component contains “dipicolinic acid”?

(A) Endospore

(B) Capsule

(C) Flagella

(D) Pili

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JAM 2022 BIOTECHNOLOGY - BT

Q.5 The fossilization process in which mineral rich water penetrates through the
pores of decomposed organic matter is known as _____.

(A) Carbonization

(B) Chemical fossilization

(C) Petrifaction

(D) Microfossilization

Q.6 A random fluctuation in gene frequency is called

(A) Genetic drift

(B) Genetic load

(C) Panmixis

(D) Genetic shift

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JAM 2022 BIOTECHNOLOGY - BT

Q.7 The number of “Barr Bodies” present in a somatic cell of a woman suffering
from Turner syndrome is ____.

(A) 0

(B) 1

(C) 2

(D) 3

Q.8 Which of the following are produced by Mangrove trees to survive in the
waterlogged swampy forests?

(A) Trichomes

(B) Pneumatophores

(C) Spermatophores

(D) Cambia

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JAM 2022 BIOTECHNOLOGY - BT

Q.9 Indeterminate growth in plants is due to the presence of perpetually
undifferentiated tissues, called as ______.

(A) Tracheids

(B) Meristems

(C) Parenchyma

(D) Sclerenchyma

Q.10 The osmotic potential (ψ) of pure water is _____ MPa.

(A) -1

(B) 0

(C) 0.1

(D) 10

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JAM 2022 BIOTECHNOLOGY - BT

Section A: Q.11 – Q.30 Carry TWO marks each.

Q.11 Bacteria containing a tuft of flagella that comes out from one pole is called ___.

(A) Lophotrichous

(B) Peritrichous

(C) Monotrichous

(D) Amphitrichous

Q.12 Which of the following activity is associated with Klenow fragment?

(A) 5′-3′ exonuclease activity

(B) 5′-3′ endonuclease activity

(C) Polymerase activity

(D) 3′-5′ endonuclease activity

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JAM 2022 BIOTECHNOLOGY - BT

Q.13 A frameshift mutation is caused by ____.

(A) 5-Bromouracil

(B) Acridine

(C) Glutathione

(D) Hypoxanthine

Q.14 The zone of a pond system where respiration is more than production is called
as _____.

(A) Limnetic zone

(B) Littoral zone

(C) Epilimnion zone

(D) Benthic zone

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JAM 2022 BIOTECHNOLOGY - BT

Q.15 An organism that causes obstruction of lymphatic system in humans is ____.

(A) Borrelia burgdorferi

(B) Brucella abortus

(C) Yersinia pestis

(D) Wuchereria bancrofti

Q.16 A man having a dominant genetic trait (TT genotype) can taste
phenylthiocarbamide (PTC), marries a woman who cannot taste PTC. The PTC
tasting ability of their biological son and daughter is

(A) Son taster; Daughter non-taster

(B) Daughter taster; Son non-taster

(C) Both are non-tasters

(D) Both are tasters

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JAM 2022 BIOTECHNOLOGY - BT

Q.17 Which of the following enzymes is absent in a person suffering from
Alkaptonuria?

(A) Tyrosinase

(B) Homogentisic acid oxidase

(C) Catechol dioxygenase

(D) Phenylalanine hydroxylase

Q.18 The bacterium that can tolerate high concentrations of salt and also ferment
mannitol is

(A) Staphylococcus aureus

(B) Staphylococcus epidermis

(C) Streptococcus pyogenes

(D) Serratia marcescens

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JAM 2022 BIOTECHNOLOGY - BT

Q.19 Match the following

Group I Group II

P) Streptomycin 1) Inhibits beta-subunit of RNA polymerase

Q) Cycloheximide 2) Inhibits peptidyl transferase activity of 50S subunit

R) Rifamycin 3) Inhibits peptidyl transferase activity of 60S subunit

S) Chloramphenicol 4) Inhibits binding of formyl methionine tRNA to ribosome

(A) P-1, Q-3, R-4, S-2

(B) P-4, Q-3, R-1, S-2

(C) P-2, Q-3, R-1, S-4

(D) P-3, Q-4, R-1, S-2

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JAM 2022 BIOTECHNOLOGY - BT

Q.20 The major product formed in the given reaction is

(A)

(B) t
O
Bu

(C)

(D)

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JAM 2022 BIOTECHNOLOGY - BT

Q.21 DNA gyrase can

(A) cut single-stranded DNA

(B) relax supercoiled DNA

(C) introduce negative supercoiling in DNA

(D) not utilize ATP

Q.22 The stationary phase of cation-exchange chromatography can be

(A) DEAE-cellulose

(B) CM-cellulose

(C) Sephadex G-50

(D) Heparin-Sepharose

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JAM 2022 BIOTECHNOLOGY - BT

Q.23 Components of a Transmission Electron Microscope are

(A) Electron gun, objective lens, positron beam, projector lens

(B) Neutron beam, projector lens, objective lens, evacuated tube

(C) Electron beam, projector lens, objective lens, condenser lens

(D) X-ray beam, projector lens, objective lens, condenser lens

Q.24 In a honey bee population, the workers are infertile but protect the queen from
intruders and help in reproduction. This is an example of

(A) K selection

(B) Sexual selection

(C) Kin selection

(D) Disruptive selection

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JAM 2022 BIOTECHNOLOGY - BT

Q.25 For an enzyme following Michaelis-Menten kinetics, when [S]=KM then, the
velocity v is

([S] is substrate concentration, KM is Michaelis constant, Vmax is maximal
velocity)

(A) [S] × Vmax

(B) 0.75 × Vmax

(C) 0.5 × Vmax

(D) KM × Vmax

Q.26 The net equation for aerobic glycolysis is

(A) Glucose+2ATP 2 lactate+2ADP+2Pi

(B) Glucose+2ADP+2Pi+2NAD+ 2 pyruvate+2ATP+2NADH+2H2O+4H+

(C) Glucose+2ADP+2Pi 2 pyruvate+2ATP+2H2O

(D) Glucose+2ADP+2Pi+2NAD+ 2 lactate+2ATP+2NADH+2H2O+4H+

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JAM 2022 BIOTECHNOLOGY - BT

Q. 27 In the electron transport chain, flavin mononucleotide (FMN) can adopt ______
as the highest oxidation state and is capable of accepting or donating _____
electrons, respectively.

(A) 2; 2 or 3

(B) 2; 1 or 2

(C) 3; 2 or 3

(D) 3; 1 or 2

Q.28 In bacteria, the σ factor that plays a major role in transcription during the
stationary phase is

(A) σ70

(B) σ54

(C) σ28

(D) σ32

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JAM 2022 BIOTECHNOLOGY - BT

Q.29 A rise in cytosolic calcium ion concentration just after fertilization in a sea
urchin egg leads to

(A) Formation of fertilization envelope

(B) Acrosomal reaction

(C) Formation of vegetal pole

(D) Formation of animal pole

Q.30 In a nephron, _____ follows the ascending limb of the “loop of Henle”.

(A) Descending limb

(B) Distal tubule

(C) Collecting tubule

(D) Proximal tubule

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JAM 2022 BIOTECHNOLOGY - BT

Section B: Q.31 – Q.40 Carry TWO marks each.

Q.31 Transpirational pull that extends down to the roots in plants can be interrupted
by

(A) Process of cavitation

(B) Process of gravitation

(C) Formation of water vapor pockets

(D) Positive pressure in xylem sap

Q.32 Transfer of plasmids into animal cells can be achieved by

(A) Electroporation

(B) Liposome-mediated process

(C) Calcium chloride treatment

(D) Sucrose treatment

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JAM 2022 BIOTECHNOLOGY - BT

Q.33 Archaeal cell membranes contain lipids that are

(A) Ether linked

(B) Ester linked

(C) Branched alkyl chain

(D) Linear alkyl chain

Q.34 Which of the following are producers in an ecological system?

(A) Macrophytes

(B) Phytoplanktons

(C) Zooplanktons

(D) Cyanobacteria

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JAM 2022 BIOTECHNOLOGY - BT

Q.35 Which of the following acts as wound hormones in plants?

(A) Ethylene

(B) Cytokinins

(C) Abscisic acid

(D) Dextrin

Q.36 The enriched media used to facilitate the growth of fastidious microorganisms
are

(A) Selenite F broth

(B) Blood agar

(C) Chocolate agar

(D) Loeffler’s serum

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JAM 2022 BIOTECHNOLOGY - BT

Q.37 Match the bacterial structure to function

(i) Cell wall (a) Virulence factor

(ii) Glycocalyx (b) Selective permeability

(c) Attachment to surfaces

(d) Protection from osmotic lysis

(A) (i)-(b), (ii)-(d)

(B) (i)-(d), (ii)-(a)

(C) (i)-(c), (ii)-(b)

(D) (i)-(d), (ii)-(c)

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JAM 2022 BIOTECHNOLOGY - BT

Q.38 Identify the correct pairs:

(i) Thermophile (a) grows optimal at 37 °C

(ii) Mesophile (b) grows optimal at low temperature

(iii) Psychrophile (c) grows optimal at high saline conditions

(iv) Halophile (d) grows optimal at 67 °C

(A) (i)-(d)

(B) (ii)-(b)

(C) (iii)-(a)

(D) (iv)-(c)

Q.39 A single copy of an allele in sickle-cell heterozygous individuals reduces the
frequency and severity of malaria. The reason for this is

(A) Low oxygen binding capacity of hemoglobin

(B) Single amino acid substitution in hemoglobin deforms the red blood cells

(C) Abnormal hemoglobin is toxic for malaria parasite

(D) Malaria parasite escapes the deformed red blood cells

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JAM 2022 BIOTECHNOLOGY - BT

Q.40 The correct statement/s for bimolecular nucleophilic substitution reactions is/are

(A) It goes through a carbocation formation

(B) There is an inversion of configuration if the reacting center is chiral

(C) Reaction is enhanced when carried out in polar solvents

(D) The reaction intermediate is trigonal bipyramidal

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JAM 2022 BIOTECHNOLOGY - BT

Section C: Q.41 – Q.50 Carry ONE mark each.

Q.41 A deck of ten cards is given to you as shown below in the figure. One card is
drawn at random from this deck. The probability of selecting a number less than
9 is ____. (to one decimal place)

Q.42 The average of all positive even integers less than or equal to 40 is ___.

Q.43 The smallest positive (non-zero) integer “n” for which the expression

1+𝑖 𝑛
(1−𝑖) = 1 holds true, is ___.

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JAM 2022 BIOTECHNOLOGY - BT

Q.44 Given that

A= (𝑠𝑖𝑛Ө 𝑐𝑜𝑠Ө 𝑡𝑎𝑛Ө + 𝑠𝑖𝑛Ө 𝑐𝑜𝑠Ө 𝑐𝑜𝑡Ө), the value of A is

Q.45 An object is placed at the principal focus of a concave lens of focal length 10
cm. The image will be formed at _____cm, between the optical center and the
focus of the lens on the same side of the object.

Q.46 What is the maximum number of hydrogen bonds that a water molecule can
make in the liquid state?

Q.47 How many pairs of autosomal chromosomes are there in normal humans?

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JAM 2022 BIOTECHNOLOGY - BT

Q.48 Calculate the temperature (in K) at which the resistance of a metal becomes
20% more than its resistance at 300 K. The value of the temperature coefficient
of resistance for this metal is 2.0 × 10-4 /K.

Q.49 In the 1H NMR spectrum of ethanol at 400 MHz, the methyl group splits into
_________ number of peaks.

Q.50 In a denaturing polyacrylamide gel electrophoresis experiment, pure intact adult
human hemoglobin will yield ________(number) bands.

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JAM 2022 BIOTECHNOLOGY - BT

Section C: Q.51 – Q.60 Carry TWO marks each.

Q.51 A man throws a ball vertically up in the air with an initial velocity v1 such that it
reaches a height of 12 m with a speed of 12 m/s. If he throws the same ball
vertically up with an initial velocity v2 such that it reaches a maximum height of
12 m. Calculate v1/v2. (up to 2 decimal places)

Q.52 What is the acceleration due to gravity (m/s2) on the surface of a planet if its
radius is 1/4th that of earth and its mass is 1/80th that of earth? Assume that the
gravity on the surface of the earth is 10 m/s2.

Q.53 In a randomly mating population, the frequency of ‘A’ allele is 0.7. What is the
frequency of ‘Aa’ genotype in the next generation according to Hardy-
Weinberg’s law? (up to two decimal places)

Q.54 The potential difference to accelerate an electron was quadrupled. By what
factor does the de Broglie wavelength of the electron beam change?

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JAM 2022 BIOTECHNOLOGY - BT

Q.55 A 500 nm light is used for imaging in a confocal microscope. What will be the
best resolution (in nm) of this microscope?

Q.56 Assuming the molecule shown below is aromatic, the value of “n” according to
“Hückel’s rule” is

Q.57 In an actively growing population from a single bacterium, 1,048,576 cells are
present after 20th generation. How many cells were there in 5th generation?

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Q.58 A double stranded DNA molecule of total 5000 base pairs long, has a melting END
temperature of 85 °C. What will be the % AT base pairs in this sample? (up OF
THE
to one decimal place).

Q.59 How many GTP molecules are required for the translocation of tRNA from P
site to E site during translation elongation process in bacteria?

Q.60 Amongst the molecules given below, the total number of molecules that have at
least one sp2 hybridized atom is __________.

C6H6, NO2, BF3, H2O2, SO2, C2H2, L-Tryptophan

QUESTION PAPER

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