TN 12th Question Paper 2023 Microbiology

Tamil Nadu
State Board

2023
QUESTION
PAPER

No. of Printed Pages : 8
6662
!6662Microbiology! £vÄ Gs
Register Number

PART - III
~sq°›¯À / MICROBIOLOGY
( uªÌ ©ØÖ® B[Q» ÁÈ / Tamil & English Version)

Põ» AÍÄ : 3.00 ©o ÷|µ® ] [ ö©õzu ©v¨ö£sPÒ : 70

Time Allowed : 3.00 Hours ] [ Maximum Marks : 70

AÔÄøµPÒ : (1) AøÚzx ÂÚõUPЮ \›¯õP £vÁõQ EÒÍuõ GߣuøÚ \›£õºzxU
öPõÒÍÄ®. Aa_¨£vÂÀ SøÓ°¸¨¤ß AøÓU PsPõo¨£õÍ›h®
EhÚi¯õPz öu›ÂUPÄ®.
(2) }»® AÀ»x P¸¨¦ ø©°øÚ ©mk÷© GÊxÁuØS®,
AiU÷PõikÁuØS® £¯ß£kzu ÷Ásk®. £h[PÒ ÁøµÁuØS
ö£ß]À £¯ß£kzuÄ®.
(3) ÷uøÁ¯õÚ Ch[PÎÀ £h[PÒ ÁøµP.
Instructions : (1) Check the question paper for fairness of printing. If there is any lack of
fairness, inform the Hall Supervisor immediately.
(2) Use Blue or Black ink to write and underline and pencil to draw
diagrams.
(3) Draw diagrams wherever necessary.

£Sv & I / PART - I
SÔ¨¦ : (i) AøÚzx ÂÚõUPÐUS® Âøh¯ÎUPÄ®. 15x1=15

(ii) öPõkUP¨£mkÒÍ ©õØÖ ÂøhPÎÀ ªPÄ® Hئøh¯ Âøhø¯z
÷uº¢öukzxU SÔ±mkhß Âøh°øÚ²® ÷\ºzx GÊuÄ®.
Note : (i) Answer all the questions.

(ii) Choose the most appropriate answer from the given four alternatives
and write the option code and the corresponding answer.

[ v¸¨¦P / Turn over

6662 2

1. Jΰ¯À ¤®£ öuõÈÀ ~m£zvØS __________ J¸ Euõµn®.
(A) CLSM (B) LCSM
(C) (A) ©ØÖ® (B) (D) TEM
__________ is an example for optical imaging technique.
(a) CLSM (b) LCSM
(c) Both (a) and (b) (d) TEM

2. ö£õ¸zxP :
(1) SEM (i) C¸ £›©õn ¤®£®
(2) TEM (ii) \õ¯÷©ØÓ¨£hõu E°¸ÒÍ ö\ÀPÒ
(3) ¦÷Íõµ\ßì ~s÷nõUQ (iii) •¨£›©õn ¤®£®
(4) PCM (iv) ø©U÷Põ£õUj›¯® j³£ºS÷Íõ]ì
(A) (1)-(i), (2)-(iii), (3)-(iv), (4)-(ii)
(B) (1)-(i), (2)-(ii), (3)-(iv), (4)-(iii)
(C) (1)-(iii), (2)-(i), (3)-(iv), (4)-(ii)
(D) (1)-(iii), (2)-(ii), (3)-(iv), (4)-(i)
Match the following :
(1) SEM (i) 2 dimensional image
(2) TEM (ii) Unstained living cell
(3) Fluorescence microscope (iii) 3 dimensional image
(4) PCM (iv) Mycobacterium tuberculosis
(a) (1)-(i), (2)-(iii), (3)-(iv), (4)-(ii)
(b) (1)-(i), (2)-(ii), (3)-(iv), (4)-(iii)
(c) (1)-(iii), (2)-(i), (3)-(iv), (4)-(ii)
(d) (1)-(iii), (2)-(ii), (3)-(iv), (4)-(i)

3. __________ CÀ 10 •uÀ 20 {ªh[PÐUS ‰ÌP ö\´Áuß ‰»® öuº©õ«mhºPÒ
~sq°º }UP® ö\´¯¨£kQßÓÚ.
(A) A÷¯õiß (B) BÀPíõÀ (C) ö©ºS› (D) S÷Íõøµk
Thermometers are disinfected by immersing in __________ for 10 to 20 minutes.
(a) Iodine (b) Alcohol (c) Mercury (d) Chloride

4. ¤ßÁ¸® PõµoPÎÀ Gx Ps ©¸¢øu £u¨£kzxÁuØS £¯ß£kQÓx ?
(A) |õßQøn¨¦ A®÷©õÛ¯® E¨¦ (B) ¥ÚõÀ
(C) Gzv¼ß BUøék (D) BÀiøíkPÒ
Which among the following agent is used as a preservative in ophthalmic solution ?
(a) Quaternary ammonium salt (b) Phenol

(c) Ethylene Oxide (d) Aldehydes

3 6662

5. ¤ßÁ¸ÁÚÁØÔÀ \›¯õÚx Gx ?
(A) A÷£õGßø\® + ÷íõ÷»õGßø\® = ÷PõGßø\®
(B) A÷£õGßø\® + ÷PõL÷£Uhº = ÷íõ÷»õGßø\®
(C) ÷íõ÷»õGßø\® + ÷PõGßø\® = A÷£õGßø\®
(D) ÷PõGßø\® + ÷PõL÷£Uhº = ÷íõ÷»õGßø\®
Which one of the following is correct ?
(a) Apoenzyme + Holoenzyme = Coenzyme
(b) Apoenzyme + Cofactor = Holoenzyme
(c) Holoenzyme + Coenzyme = Apoenzyme
(d) Coenzyme + Cofactor = Holoenzyme

6. £õ¼À __________ ÷P]ß ¦µu® EÒÍx.
(A) 50 - 100% (B) 90 - 95% (C) 60 - 65% (D) 80 - 85%
Milk contains about ___________ of casein protein.
(a) 50 - 100% (b) 90 - 95% (c) 60 - 65% (d) 80 - 85%

7. ì- ÷ £õº EØ£zv ö\´²® ~sq°ºPøÍ £u¨£kzu __________ •øÓ
£¯ß£kQÓx.
(A) Põ¯øÁzuÀ (B) E»ºzxuÀ
(C) vµÁ ø|mµáß (D) APõº \õ´Ä
__________ method is used for preservation of sporulating micro-organisms.
(a) Drying (b) Desiccation
(c) Liquid Nitrogen (d) Agar slopes

8. ‰øÍ uskÁh Põ´a\ø» EshõUS® £õUj›¯õ __________.
(A) \õÀ÷©õöÚÀ»õ øh¤ (B) ø|^›¯õ ö©Ûßöáiì
(C) PõºÛ£õUj›¯® i¨w›÷¯ (D) ¨›÷¯õ Põ»÷µ
The bacteria which causes cerebrospinal fever __________.
(a) Salmonella typhi (b) Neisseria meningitides

(c) Corynebacterium diphtheriae (d) Vibrio cholerae

[ v¸¨¦P / Turn over

6662 4

9. ©¸zxÁ ø©UPõ»â°ß u¢øu :
(A) ÷áõ\¨ ¼ìhº (B) ¿°ì £õìa\º
(C) µõ£ºm ÷Põa (D) ÷µ©sm ÷áUì \õ÷£õµõm
The father of Medical Mycology is :
(a) Joseph Lister (b) Louis Pasteur
(c) Robert Koch (d) Raymond Jacques Sabouraud

10. __________ §gø\ ‰»® Aøn¯õøh ÷uõÀ AÇØ] HØ£kQÓx.
(A) ö£Û]½¯® ö|õm÷hh®
(B) Q›¨÷hõPõUPì {÷¯õ£õº©ßì
(C) ÷Pßihõ BÀ¤Pßì
(D) îì÷hõ¤Íõì©õ ÷P¨_÷»mh®
Diaper rash is caused by the Fungi __________.
(a) Penicillium notatum
(b) Cryptococcus neoformans
(c) Candida albicans
(d) Histoplasma capsulatum

11. Jmkso°ß £õ¼Ú CÚ¨ö£¸UP •øÓø¯ ÷©ØöPõÒЮ ¸¢÷uõ®¤
__________ BS®.
(A) Phzx® ¸¢÷uõ®¤ (B) {ø»¯õÚ Â¸¢÷uõ®¤
(C) ÷uUS® ¸¢÷uõ®¤ (D) Cøh{ø» ¸¢÷uõ®¤
A host in which a parasite undergoes sexual reproduction is __________.
(a) Transport Host (b) Definite Host
(c) Reservoir Host (d) Intermediate Host

12. EÒÐÖ¨¦ ½è÷©Û¯õ]ì Gߣx C¢v¯õÂÀ __________ GÚ
AøÇUP¨£kQÓx.
(A) Põ»õ&A\õº (B) B]¯õ Põ´a\À
(C) h®&h® Põ´a\À (D) ÷©ØTÔ¯ AøÚzx®
In India, Visceral Leishmaniasis is called as __________.

(a) Kala-Azar (b) Asian fever
(c) Dum-Dum fever (d) All of the above

5 6662

13. TØÖ (A) : ø\m÷hõö©P÷»õ øÁµì, ¥mhõ öíº¨£ì øÁµéõP ÁøP¨&
£kzu¨£mkÒÍx.
Põµn® (R) : ¥mhõ öíº¨£ì øÁµì SÖQ¯ CµmizuÀ _ÇØ] ©ØÖ®
£À÷ÁÖ K®¦|ºPøÍ öPõskÒÍx.
(A) TØÖ (A) Gߣx \› ©ØÖ® Põµn® (R), TØøÓ (A) ÂÍUSQÓx
(B) TØÖ (A) Gߣx \›, Põµn® (R) Gߣx uÁÖ
(C) TØÖ (A) ©ØÖ® Põµn® (R) BQ¯ Cµsk® uÁÖ
(D) TØÖ (A) Gߣx uÁÖ, Põµn® (R) Gߣx \›
Assertion (A) : Cytomegalo virus is classified as Beta Herpes virus.
Reason (R) : Beta Herpes virus have short replicative cycle and variable host
range.
(a) Assertion (A) is true and Reason (R) explains assertion (A)
(b) Assertion (A) is true, Reason (R) is false

(c) Both Assertion (A) and Reason (R) are false
(d) Assertion (A) is false, Reason (R) is true

14. •ußø© Bµ®£ ÷Põhõß AUG, __________ Aª÷Úõ Aª»zøu SÔUQßÓx.
(A) «zv÷¯õøÚß (B) BºâøÚß
(C) I÷\õ¼²]ß (D) Áõø»ß
The principal start codon AUG, specifies the amino acid :
(a) Methionine (b) Arginine

(c) Isoleucine (d) Valine

15. __________, ÁøP II ªøP TºEnºÄ ÂøÚ°À Dk£kQÓx.

(A) IgM (B) IgG
(C) IgE (D) IgG ©ØÖ® IgM
In type II hypersensitivity reaction __________ is involved.
(a) IgM (b) IgG

(c) IgE (d) IgG and IgM

[ v¸¨¦P / Turn over

6662 6

£Sv & II / PART - II
SÔ¨¦ : GøÁ÷¯Ý® BÖ ÂÚõUPÐUS Âøh¯ÎUPÄ®. ÂÚõ Gs 24 &US
Pmhõ¯©õP Âøh¯ÎUPÄ®. 6x2=12

Note : Answer any six questions. Question No. 24 is Compulsory.

16. |õ÷Úõ xPÒPÎß £¯ß£õkPÒ H÷uÝ® |õßQøÚ GÊxP.
Write any four applications of Nanoparticles.

17. G»Umµõß ~s÷nõUQ°ß ÁøPPÒ ¯õøÁ ?
What are the different types of Electron microscope ?

18. Bßi£¯õiU – Áøµ¯ÖUPÄ®. H÷uÝ® Cµsk GkzxUPõmkPÒ u¸P.
Define Antibiotic. Give any two examples.

19. QøÍPõøÍ]ì – Áøµ¯ÖUPÄ®.
Define Glycolysis.

20. £õv ö\¯ØøP¯õÚ ö£Û]¼ß GßÓõÀ GßÚ ? GkzxUPõmk u¸P.
What is meant by semi-synthetic penicillin ? Give an example.

21. ShØPõ´a\À GßÓõÀ GßÚ ?
What is Enteric fever ?

22. A«¤U ^u÷£v°ß ©¸zxÁ öÁΨ£õkPÒ ¯õøÁ ?
What are the clinical features of Amoebic dysentry ?

23. ÷hß xPÒ – Áøµ¯ÖUPÄ®.
Define Dane Particle.

24. PCR öuõÈÀ~m£zvÀ £¯ß£kzu¨£k® Cµsk ¤µ£»©õÚ ö|õvPøÍU TÖP.

State the two popular enzymes, employed in the PCR technique.

7 6662

£Sv & III / PART - III
SÔ¨¦ : GøÁ÷¯Ý® BÖ ÂÚõUPÐUS Âøh¯ÎUPÄ®. ÂÚõ Gs 33 &US
Pmhõ¯©õP Âøh¯ÎUPÄ®. 6x3=18
Note : Answer any six questions. Question No. 33 is Compulsory.
25. ¦÷Íõµ\ßì ~s÷nõUQ°À £¯ß£kzu¨£k® £À÷ÁÖ ÁøP¯õÚ ÁiPmiPÒ
¯õøÁ ?
What are the different types of filters used in Fluorescence microscope ?

26. Áͺ©õØÓ® ©ØÖ® ]øu©õØÓzvØQøh÷¯ EÒÍ ÷ÁÖ£õkPøÍ GÊxP.
Write the differences between Anabolism and Catabolism.

27. EnĨ £u¨£kzxu¼ß |ßø©PÒ ©ØÖ® wø©PÒ ¯õøÁ ?
What are the advantages and disadvantages of Food Preservation ?

28. •ußø© ÷uºÄ •øÓ°øÚ Euõµnzxhß Áøµ¯ÖUPÄ®.
Define primary screening with example.

29. ÷PßPº – Áøµ¯ÖUPÄ®.
Define Chancre.

30. ÷|º•P ©ØÖ® ©øÓ•P ÁõÌUøP _ÇØ] GßÓõÀ GßÚ ? GkzxUPõmk u¸P.
What is meant by Direct and Indirect life cycle ? Give an example.

31. öh[S Cµzu P]Ä Põ´a\¼ß AÔSÔPÒ ¯õøÁ ?
What are the symptoms of Dengue Hemorrhagic fever ?

32. EÖ¨¦ ©õØÓ® GßÓõÀ GßÚ ? EÖ¨¦ ©õØÓzvß •øÓPøͨ £mi¯¼kP.
What is meant by Transplantation ? List the methods of Transplantation.

33. §gø\ øh©õº¤\® GßÓõÀ GßÚ ? GkzxUPõmk u¸P.

What is Fungal Dimorphism ? Give an example.

[ v¸¨¦P / Turn over

6662 8

£Sv & IV / PART - IV
SÔ¨¦ : AøÚzx ÂÚõUPÐUS® Âøh¯ÎUPÄ®. 5x5=25
Note : Answer all the questions.
34. (A) ÷£ì Põßmµõìm ~s÷nõUQ°ß (PCM) ö\¯À C¯[Pø©¨ø£¨
£hzxhß ÂÁ›UPÄ®.
AÀ»x
(B) Qº¤ £õ¯º & Ámhz uPk £µÁÀ •øÓ ‰»® Bßi£¯õiU Tº EnºÄ
÷\õuøÚø¯ ÂÁ›UPÄ®.
(a) Explain the working mechanism of Phase Contrast Microscope with a neat
diagram.
OR
(b) Explain antibiotic sensitivity test through Kirby-Bauer Disc Diffusion method.

35. (A) TCA _ÇØ]ø¯ ÂÁ›UPÄ®.
AÀ»x
(B) EnÄ |g\õuÀ – ÁøP¨£kzxP.
(a) Explain TCA cycle.
OR
(b) Classify Food poisoning.

36. (A) ]m›U Aª» u¯õ›¨¤À EÒÍ £i{ø»PøÍ ÂÁ›UPÄ®.
AÀ»x
(B) Põ»µõ |a_ ö\¯À£k® •øÓ £ØÔ ÂÁ›UPÄ®.
(a) Explain the steps involved in Citric Acid Production.
OR
(b) Explain the mode of action of Cholera toxin.

37. (A) AìPõ›ì »®¤›Põ´öhì]ß ÁõÌUøP _ÇØ]ø¯ ÂÁ›UPÄ®.
AÀ»x
(B) ÷µ¥ì øÁµêß Aø©¨ø£ ÂÍUPÄ®.
(a) Explain the life cycle of Ascaris lumbricoides.
OR
(b) Explain the structure of Rabies Virus.

38. (A) EhÚi ©ØÖ® Põ»® uõÌzv¯ ªøP Tº EnºÄPÐUS Cøh÷¯ EÒÍ
÷ÁÖ£õkPÒ ¯õøÁ ?
AÀ»x
(B) H®ì ÷\õuøÚø¯ ÂÁ›UPÄ®.
(a) What are the differences between Immediate and Delayed Type
hypersensitivity reaction.

OR
(b) Explain Ames test.
-oOo-