PU CET UG 2015 Question Paper Biotechnology

CET-2015 Sr. No. : _1_5_0_1_2_5 _I
Booklet Series Code : A
Important: Please consult your Admit Card / Roll No. Slip before filling your Roll Number on the Test Booklet and
Answer Sheet.
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O.M.R. Answer Sheet Serial No.
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Subject : BIOTECHNOLOGY
Time: 70 minutes Number of Questions : 60 Maximum Marks: 120
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,.: '.

Biotechnology/A

1. During bacterial conjugation, Fertility factor "F" is carried by :
(A) Male (B) Female

(C) Both by male and female (D) Neither by male nor by female

2. Chromatin consists of :
(A) histonesonly (B) DNAandhistonesonly.
(C) histones and other proteins only (D) DNA, histones, and other proteins.

3. A relationship among alleles where both alleles contribute to the phenotype ofthe heterozygote
is called:
(A) Dominance (B) Co-dominance
(C) Incomplete dominance (D) Pureline selection

4. When one gene influences multiple, seemingly unrelated phenotypic traits, it is known as :
(A) Pleiotropy (B) Epistasis

(C) Methylation (D) Epigenetics

5. Satellite DNA is typically found in :
(A) Centromeres (B) Heterochromatin

(C) Both centromeres and heterochromatin (D) Telomeres

6. Expression vector differs from normal cloning vector in having:

(A) An origin of replication (B) Suitable marker genes
(C) Unique restriction sites (D) Control elements

7. Which ofthe following molecules contains an anticodon ?
(A) rnRNA (B) tRNA
(C) rRNA (D) protein
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8. The 02 evolved in photosynthes~s comes from:

(A) carbon dioxide (B) water

(C) glucose (D) (C~O)

9. Which pigment is present in all photosynthetic eukaryotes ?

(A) Chlorophyll a (B) Chlorophyll b
(C) Chlorophyll c (D) Bacteriochlorophyll

10. In pBR322, pBR stands for:
(A) Plasmid bacterial recombination (B) Plasmid bacterial replication

(C) Plasmid bolivar and rodriguez (D) Plasmid baltimore and rodriguez

11. results in the production ofRNA using a DNA template.

(A) Replication (B) Transcription
(C) Translation (D) Amplification

12. Which ofthe following does not follow base pairing rules?
(A) A:T (B) U:T
(C) G: C (D) U:A

13. Karyogamy is the:
(A) Fusion of protoplasts (B) Formation of a dikaryon
(C) Fusionofnuc1ei (D) Formationofrhizoids

14. All ofthe following are matched correctly except:
(A) Myosin: motor protein (B) Keratin: immune defense proteins

(C) Hemoglobin: transport protein (D) Collagen: structural protein

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15. Insulin is an example of a protein.

(A) Storage (B) Regulatory

(C) Protective (D) Transport

16. Alternative versions of the same single-copy gene are:

(A) Alleles (B) Mosaic genes

(C) Pseudogenes (D) Regulatory genes

17. The process of photosynthesis result in the formation of two substances essential to our

existence:
(A) Chlorophyll and water (B) Sugar and oxygen

(C) Sugar and water (D) Chlorophyll and oxygen

18. The most abundant organic molecule in nature are:
(A) Proteins (B) Carbohydrates

(C) Lipids (D) Nuc1eicacids

19. first concluded that all cells arise from preexisting cells.

(A) Theodor Schwarm (B) Robert Hooke
(C) Charles Darwin (D) RudolfVirchow

20. The Haber-Bosch process is important in the:

(A) Reduction of soil erosion
(B) Development of pesticides
(C) Manufacture of fertilizers

(D) Development of efficient irrigation systems

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21. Eukaryotic ceUs differ from prokaryotic cells in that eukaryotic cells have:

(A) Anuc1eus (B) A cytoplasm

(C) Aplasmamembrane CD) Genetic material

22. The nucleolus is the structure in which ---- are formed.

(A) Nuclear pores (B) Chromosomes

(C) Ribosomes CD) UnitesofER

23. Grana are stacks of within chloroplast.

(A) Prolamel1arbodies (B) Thylakoids

(C) Stroma (D) Etioplasts

24. In the cell cycle, interphase consists of:
(A) Mitosis and cytokinesis (B) Mitosis and the S phase

(C) The Gland G2 phase (D) The G 1, S and G2 phase

25. During the nuclear envelopes and nucleoli reforms.
(A) Anaphase (B) Metaphase
(C) Prophase (D) Telophase

26. The chief reservoir of nitrogen is :

(A) the ocean (B) livingorganisms
(C) dead organic material (D) the atmosphere

27. Nitrite is oxidized to nitrate by :

(A) Nitrosomonas (B) Nitrobacter

(C) ammonifying bacteria and fungi (D) d. denitrifying bacteria

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28. Water potential is defined as :

(A) tendency of water to enter the cell (B) tendency of water to leave the cell

(C) kinetic energy of water (D) potential energy of water

29. Mutualism is an interaction between two species in which:

(A) one species benefits and the other is harmed

(B) both species benefit

(C) both species are harmed

(D) one species benefits and the other is neither hanned nor helped

30. If the concentration of K + is higher outside a plant cell than inside, K + will enter the
cell by:
(A) facilitated diffusion through channel proteins (B) facilitated diffusion via carrier proteins

(C) active transport through channel proteins (D) active transport via carrier proteins

31. Which of the following would NOT occur during signal recognition?

(A) The signal molecule binds to a specific receptor on the plasma membrane.

(B) The signal molecule is transported into the cell by endocytosis.

(C) The signal molecule is transported out of the cell by exocytosis.

(D) The signal molecule remains outside the celL

32. In biological reactions, when a molecule is oxidized it an electron and
a(n) _

(A) loses; proton (B) gains; proton

(C) loses; oxygen atom (D) gains; oxygen atom

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33. A substrate binds to its enzyme at a location called the site.

(A) coenzyme (B) substrate

(C) active (D) polypeptide

34. Isozymes are:

(A) RNA molecules that catalyze metabolic reactions
(B) identical coenzymes that require different metal ions
(C) identical coenzymes located in different parts of the cell

(D) different enzymes that catalyze identical reactions

35. Which ofthe following statements concerning the effect of pH on enzyme activity is FALSE?
(A) The pH affects positively-charged amino acids

(B) The pH affects negatively-charged amino acids
(C) The binding capacity of an enzyme is affected by pH

(D) Enzymes are always present at their pH optimum

36. In an ATP molecule, phosphoanhydride bonds link:
(A) adenine to ribose (B) adenine to a phosphate group

(C) the phosphate groups together (D) ribose to a phosphate group

37. In feedback inhibition, the enzyme in a metabolic pathway is inhibited by
the -----

(A) last; end product (B) first; cofactor
(C) first; first substrate (D) first; end product

38. Formation of ATP from ADP and phosphate as a result of electron transport occurs in :

(A) the formation of acetyl CoA (B) fermentation
(C) glycolysis (D) oxidative phosphorylation

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39. In respiration, most ofthe energy in the original glucose molecule is :
(A) stored in molecules ofADP (B) stored in molecules ofATP
(C) released in molecules of carbon dioxide (D) released as heat

40. For every molecule of glucose that begins glycolysis, how many ATP molecules are
consumed?
(A) 0 (B) 1
(C) 2 (D) 3

41. Under anaerobic conditions, yeasts and most plant cells convert pyruvate to:
(A) acetyl Co A (B) lactate
(C) ethanol and carbon dioxide (D) ArP

42. The pairing of homologous chromosomes is called:
(A) synapsis (B) chiasma
(C) crossing-over (D) recombination

43. Crossing-over occurs during:
(A) metaphase I (B) telophase I
(C) metaphase IT (D) prophase I

44. When a particular trait appears in the F2 generation but not in the Fl generation, it is
an indication that :
(A) a monohybrid cross is involved (B) a dihybrid cross is involved
(C) true-breeding plants are involved (D) the trait is recessive

45. Term of a patent is :
(A) 7 years and no renewal (B) 7 years with possibility of renewal
(C) 20 years and no renewable (0) 20 years with possibility of renewal

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46. If two genes are linked, then by definition they:
(A) are alleles of the same gene (B) occur on the same chromosome

(C) will segregate independently (D) will undergo independent assortment

47. Polyploidy refers to the:
(A) loss of part of a chromosome (B) gain of part of a chromosome

(C) gain or loss of some chromosomes (D) gain of a complete set of chromosomes

48. Cytoplasmic inheritance in plants involves genes present in the:
(A) plastids only (B) mitochondria only

(C) cytosol and plastids (D) mitochondria and plastids

49. Data obtained by Erwin Chargaffindicated that in DNA the ratio ofnuc1eotides containing
_____ to those containing is approximately 1: 1.
(A) adenine; cytosine (B) adenine; thymine
(C) guanine;thymine (D) thymine; cytosine

50. When scientists describe the genetic code as redundant, they mean that:
(A) it becomes disorganized over time

(B) many amino acids have more than one codon

(C) some codons specify stop signals
(D) it varies with cell type

51. Which ofthefollowing statements about promoters is FALSE?

(A) They are specific nucleotide sequences of DNA
(B) They consist of three nucleotides that bind to a codon
(C) They determine the position where RNA synthesis begins

(D) They determine which DNA strand is used as a template

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52. When DNA is methylated:

(A) transcription is repressed (B) transcription is stimulated

(C) histonetailsbindDNA (D) histone tails are released from DNA

53. Interspersed repeated DNA units:

(A) tend to be smaller than 10 base pairs long
(B) constitute less than 10percent of the DNA of most multicellular organisms

(C) are identical to one another
(D) are believed to have originated from transposons

54. Suppose a plasmid containing a gene of interest plus the ampR gene is used to transform

E. coli cells. When these cells are placed on a medium containing ampicillin, what will

happen?
(A) Theywilldie (B) They will survive and grow

(C) They will form blue colonies (D) They will glow with a green color

55. When biologists speak of the "fitness" of an organism they are referring to :
(A) its beauty (B) its resistance to disease
(C) the size of its gene pool (D) the number of its surviving offspring

56. Deposition of microbes for the purpose of patenting is covered under:
(A) BudapestTreaty (B) PCT

(C) European Patent Convention (D) Strasbourg Convention

57. Technique used to separate charged molecules based on their mobility in an electric field
is known as:

(A) IonexchangeChromatography (B) Electrophoresis
(C) Isoelectro-focussing (D) Partition chromatography

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58. DNA replication is semiconservative was proved by :

(A) Harsheyand Chase (B) Meselson and Stahl
(C) Johannson (D) Watson and Crick

59. In gas chromatography, the basis for separation of the components ofthe volatile material is

the difference in :
(A) Partition coefficients (B) Conductivity

(C) Molecularwt (D) Molarity

60. Ion exchange chromatography is based on the:
(A) Electrostatic attractions (B) Electrical mobility of ionic species
(C) Adsorption chromatography (D) Partition chromatography

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Panjab University, Chandigarh
CET(UG)-2015
FINAL ANSWERS / KEY
Subject: BIOTECHNOLOGY
Booklet Series Code: A
1 2 3 4 5 6 7 8 9 10
A D B A C D B B A C

11 12 13 14 15 16 17 18 19 20
B B C B B A B B D C

21 22 23 24 25 26 27 28 29 30
A C B D D D B D B A

31 32 33 34 35 36 37 38 39 40
C A C D D C D D D C

41 42 43 44 45 46 47 48 49 50
C A D D D B D D B B

51 52 53 54 55 56 57 58 59 60
B A D B D A B B A A

Note: An 'X' in the key indicates that either the question is ambiguous or it has
printing mistake. All candidates will be given credit for this question.