CBSE Class 12 Question Paper 2023 Biotechnology (Solved)

Qu e st i o n

P a p e r
2023

Series HEFG Set-4
àíZ-nÌ H$moS>
Q.P. Code 99
amob Z§.
Roll No.

O¡d-àm¡Úmo{JH$s
BIOTECHNOLOGY
:3 : 70
Time allowed : 3 hours Maximum Marks : 70

H¥$n`m Om±M H$a b| {H$ Bg àíZ-nÌ _o§ _w{ÐV n¥ð> 19 h¢ &
àíZ-nÌ _| Xm{hZo hmW H$s Amoa {XE JE àíZ-nÌ H$moS H$mo narjmWu CÎma-nwpñVH$m Ho$
_wI-n¥>ð> na {bI| &
H¥$n`m Om±M H$a b| {H$ Bg àíZ-nÌ _| >33 àíZ h¢ &
H¥$n`m àíZ H$m CÎma {bIZm ewê$ H$aZo go nhbo, CÎma-nwpñVH$m _| àíZ H$m H«$_m§H$
Adí` {bI| &
Bg àíZ-nÌ 15 {_ZQ >H$m g_` {X`m J`m h¡ & àíZ-nÌ H$m {dVaU
10.15 ~Oo {H$`m OmEJm & 10.15 ~Oo go 10.30 ~Oo VH$ N>mÌ Ho$db
àíZ- -nwpñVH$m na H$moB© CÎma Zht {bI|Jo &

Please check that this question paper contains 19 printed pages.
Q.P. Code given on the right hand side of the question paper should be
written on the title page of the answer-book by the candidate.
Please check that this question paper contains 33 questions.
Please write down the serial number of the question in the
answer-book before attempting it.
15 minute time has been allotted to read this question paper. The
question paper will be distributed at 10.15 a.m. From 10.15 a.m. to
10.30 a.m., the students will read the question paper only and will not
write any answer on the answer-book during this period.
*
99 Page 1 of 19 P.T.O.

:

(i) 33

(ii)

(iii) : 1 16

(iv) : 17 21

(v) : 22 28

(vi) : 29 30

(vii) : 31 33

(viii) 1 1
2 3

IÊS> H$

1. ê$nm§VaOm| H$s Zrbm-ídoV M`Z VH$ZrH$ BgHo$ {Zdoer {ZpîH«$`U na AmYm[aV h¡ : 1
(a) Leu2 OrZ
(b) LacZ OrZ
(c) X-J¡b OrZ
(d) Tet-r OrZ

2. {hÝX (Hind)-III à{V~§YZ E§µOmB_ H$m gyú_Ord òmoV h¡ : 1
(a)
(b)
(c)
(d)
99 Page 2 of 19

General Instructions :
Read the following instructions carefully and follow them :
(i) This question paper contains 33 questions. All questions are compulsory.
(ii) The question paper is divided into five sections Section A, B, C, D and
E.
(iii) In Section A : Questions number 1 to 16 are Multiple Choice Questions
carrying 1 mark each.
(iv) In Section B : Questions number 17 to 21 are Very Short Answer Type
Questions carrying 2 marks each.
(v) In Section C : Questions number 22 to 28 are Short Answer Type
Questions carrying 3 marks each.
(vi) In Section D : Questions number 29 and 30 are Case-based Questions
carrying 4 marks each. Each question has sub-parts with internal choice
in one sub-part.
(vii) In Section E : Questions number 31 to 33 are Long Answer Type
Questions carrying 5 marks each.
(viii) There is no overall choice. However, an internal choice has been provided
in 1 question in Section B, 1 question in Section C, 2 questions in
Section D and 3 questions in Section E. A candidate has to attempt only
one of the alternatives in such questions.

SECTION A

1. Blue-White selection technique of transformants is based on insertional
inactivation of : 1
(a) Leu2 gene
(b) LacZ gene
(c) X-gal gene
(d) Tet-r gene

2. Microbial source of Hind III restriction enzyme is : 1
(a) Haemophilus aegyptius
(b) Haemophilus influenzae
(c) Haemophilus haemolyticus
(d) Haemophilus felis

99 Page 3 of 19 P.T.O.

3. g|Ja VH$ZrH$ Ûmam S>r.EZ.E. H$m AZwH«$_Z kmV H$aZo H$s {d{Y Ho$ {bE {ZåZ{b{IV _| go
H$m¡Z-gm H$WZ ghr h¡ ? 1
(a) .EZ.E. Ho$ Q>oånboQ> H$s Vah Cn`moJ Ûmam hr àma§^H$ H$m
{dñVma {H$`m Om gH$Vm h¡ &
(b) Mma ddNTPs ddATP, ddUTP, ddCTP VWm ddGTP H$s Amdí`H$Vm
hmoVr h¡ &
(c) S>r.EZ.E. nm°{b_aoµO E§µOmB_ H$s Amdí`H$Vm hmoVr h¡ &
(d) S>r.EZ.E. ûm¥§Ibm Ho$ {dñVma Ho$ g_mnZ Ho$ {bE ddNTP H$m {Zdoe {H$`m OmVm
h¡ &

4. MmO© [abo ì`dñWm _| H$m`©aV H$mB_mo{Q´>pßgZ E§µOmB_ _| 57, 102 VWm 195 H«$_ _| AdpñWV
E_rZmo Aåb h¢ : 1
(a) his-asp-ser
(b) his-lys-ser
(c) his-lys-asp
(d) lys-his-ser

5. BgHo$ nydm©Zw_m{ZV OrZm| H$s g§»`m 6340 h¡ VWm OrZmo_ H$m 70% ^mJ àmoQ>rZ Ho$ {bE H$moS>
H$aVm h¡ : 1
(a) `rñQ>
(b) d_© (H¥${_)
(c) IanVdma
(d) OrdmUw

6. {H$g E§µOmB_ H$s AZwnpñW{V Ho$ H$maU SCID amoJ hmoVm h¡ ? 1
(a) E{S>ZmogrZ qgWoµO
(b) E{S>ZmogrZ {S>E_rZoµO
(c) E{S>ZmogrZ \$m°ñ\o$Q>oµO
(d) E{S>ZmogrZ E_rZoµO
99 Page 4 of 19

3. Which of the following statement is not correct about DNA sequencing by
Sanger Method ? 1

(a) Primers can only be extended using the single strand DNA as
template.
(b) Four ddNTPs ddATP, ddUTP, ddCTP and ddGTP are needed.

(c) DNA polymerase enzyme is needed.
(d) ddNTPs are added to terminate chain (DNA) extending.

4. Charge relay system operating in chymotrypsin enzyme consists of
following amino acids at positions 57, 102 and 195 respectively : 1
(a) his-asp-ser
(b) his-lys-ser
(c) his-lys-asp
(d) lys-his-ser

5. Number of predicted genes is 6340 and 70% part of the genome codes for
protein in : 1

(a) Yeast

(b) Caenorhabditis worm
(c) Arabidopsis weed
(d) Bacterium E. coli

6. SCID disease is caused by absence of enzyme : 1

(a) Adenosine synthase

(b) Adenosine deaminase

(c) Adenosine phosphatase

(d) Adenosine aminase

99 Page 5 of 19 P.T.O.

7. S>r.EZ.E. Ho$ A{^a§OZ hoVw {ZH$ ñWmZm§VaU VH$ZrH$ {dH${gV H$aZo dmbo d¡km{ZH$ h¢ : 1
(a) [a½~r VWm nm°b ~J©
(b) am°g VWm OmoÝg
(c) H¡$ar _w{bg VWm nm°b ~J©
(d) ES>dS>© g|Ja

8. {ZåZ Ho$ CËnmXZ go gå~Õ h¡ : 1
(a) ñQ´>oßQ>mo_mBgrZ
(b) no{Z{gbrZ
(c) {hnoQ>mB{Q>g à{VOZ
(d) nm°br 3- ã`yQ>mBaoQ>

9. A{^ì`{º$ g§dmhH$ _| {ZåZ{b{IV _| go H$m¡Z-gm JwU hmoVm h¡ ? 1
(a)
(b) gwH|$Ðr nanmofr H$mo{eH$m _| gabVm go ñWmZm§VaU
(c) {dOmVr` àmoQ>rZ H$m A{YH$V_ CËnmXZ
(d) (a) VWm (c) XmoZm|

10. àOm{V go àmá Am{Q>©{_{gZ H$mo BgHo$ {bE Cn`moJ {H$`m OmVm h¡ : 1
(a) à{V-_bo[a`br
(b) J^©{ZamoYH$
(c) à{V-A~w©XOZr
(d) hmar

11. eH©$am, _¡{ZQ>mob VWm àmobrZ O¡go `m¡{JH$ AË`{YH$ _mÌm _| CËnÞ H$aZo dmbo nmaOrdr nmXn
hmoVo h¢ : 1
(a)
(b) AO¡{dH$ à{VKmV g{hîUw
(c) Am¡f{Y VWm AÝ` _hÎdnyU© {ÛVr`H$ CnmnM`Om| H$m A{YH$ CËnmXZ
(d) IanVdmaZmer g{hîUw
99 Page 6 of 19

7. Technique of introducing colours into DNA by Nick Translation, was
developed by : 1
(a) Rigby and Paul Berg
(b) Rous and Jones
(c) Kary Mullis and Paul Berg
(d) Edward Sanger

8. Alcaligenes eutrophus is associated with the production of : 1
(a) Streptomycin
(b) Penicillin
(c) Hepatitis antigen
(d) Poly 3-hydroxybutyrate

9. Expression vector has which of the following properties ? 1
(a) High copy number and stable
(b) Easy to transfer in eukaryote cells
(c) Maximise production of foreign protein
(d) Both (a) and (c)

10. Artemisin obtained from Artemisia species is used as 1
(a) Antimalarial
(b) Antifertility
(c) Anticarcinogenic
(d) Analgesic

11. Transgenic plants which over-express compounds like sugars, mannitol
and proline 1
(a) are pest resistant
(b) are abiotic stress tolerant
(c) produce more secondary metabolites as drugs and other important
metabolites
(d) are weedicide tolerant

99 Page 7 of 19 P.T.O.

12. dh ì`{º$ {Og_| H$maH$ (\ VIII CËnÞ H$aZo H$s j_Vm H$m A^md hmoVm h¡ , {ZåZ
AmZwd§{eH$ {dH$ma go J«ñV hmoVo h¢ : 1
(a) hr_mo\$s{b`m A
(b) hr_mo\$s{b`m B
(c) {H«$g_g amoJ
(d) njmKmV (ñQ´>moH$)
13 16 (A)
(R) (a) (b) (c)
(d)
(a) A{^H$WZ (A) Am¡a H$maU (R) XmoZm| ghr h¢ Am¡a H$maU (R), A{^H$WZ (A) H$s
ghr ì`m»`m H$aVm h¡ &
(b) A{^H$WZ (A) Am¡a H$maU (R) XmoZm| ghr h¢, naÝVw H$maU (R), A{^H$WZ (A) H$s
ghr ì`m»`m Zht H$aVm h¡ &
(c) A{^H$WZ (A) ghr h¡, naÝVw H$maU (R) µJbV h¡ &
(d) A{^H$WZ (A) µJbV h¡, naÝVw H$maU (R) ghr h¢ &
13. (A) : OrdÐì`H$ (àmoQ>moßbmñQ>) H$mo{eH$m {^{Îm {dhrZ nmXn H$mo{eH$mE± h¢ &
(R) : OrdÐì`H$m| (àmoQ>moßbmñQ>m|) H$m Cn`moJ H$m{`H$ g§H$am| Ho$ {Z_m©U _| {H$`m
Om gH$Vm h¡ & 1

14. (A) : g_mZ nyd©Om| go d§emJ{V Ho$ H$maU CËnÞ g_mZVm H$mo g_OmVVm H$hVo
h¢ &
(R) : EH$ hr ñnrerµO (Om{V) Ho$ {d{^Þ ì`{ï>`m| go àmßV S>r.EZ.E. AZwH«$_m|
_| g_mZVm Ho$ H$maU g_OmVVm n[ab{jV hmoVr h¡ & 1

15. (A) : {Zdoer VWm g§dmhH$ S>r.EZ.E. H$mo à{V~§YZ EÊS>mo Ûmam H$mQ>m
OmVm h¡ &
(R) : bmBJoO nwZ`m}JO S>r.EZ.E. AUw H$mo H$mQ> gH$Vm h¡ & 1

16. (A) : gwHo$ÝÐH$s àmoQ>rZ H$s A{^ì`{º$ Ho$ {bE H$mo dar`Vm Xr
OmVr h¡ &
(R) : H$m g§dY©Z VWm Bg_| hoa\o$a gabVm go {H$`m Om gH$Vm h¡,
H$s àmoQ>rZ H$s A{^ì`{º$ hoVw {H$`m OmVm h¡ & 1

99 Page 8 of 19

12. Person who lacks the ability to produce Factor VIII has heritable genetic
disorder : 1
(a) Haemophilia A
(b) Haemophilia B
(c) Christmas disease
(d) Stroke

Questions No. 13 to 16 consists of two statements Assertion (A) and Reason (R).
Answer these questions selecting the appropriate option given below :
(a) Both Assertion (A) and Reason (R) are true and Reason (R) is the
correct explanation of Assertion (A).
(b) Both Assertion (A) and Reason (R) are true, but Reason (R) is not
the correct explanation of Assertion (A).
(c) Assertion (A) is true, but Reason (R) is false.
(d) Assertion (A) is false, but Reason (R) is true.

13. Assertion (A) : Protoplasts are plant cells without cell wall.
Reason (R) : Protoplasts can be utilized for producing somatic hybrids. 1

14. Assertion (A) : Homology is defined as similarity due to common
ancestry.

Reason (R) : DNA sequences obtained from different individuals of
same species show homology due to similarity in DNA
sequence. 1

15. Assertion (A) : Insert and vector DNA are cut with the help of Restriction
endonuclease.

Reason (R) : Ligase can produce the nick in the recombinant DNA
molecule. 1

16. Assertion (A) : For the expression of eukaryotic proteins, E. coli is
preferred.
Reason (R) : E. coli is easy to grow and manipulate, so is used to
express eukaryotic proteins. 1

99 Page 9 of 19 P.T.O.

IÊS> I

17. (H$) g§dmhH$ S>r.EZ.E. na b¡H$Z (lacZ) OrZ `wº$ nwZ`m}JOm| Ho$ M`Z Ho$ {bE, Bgo
H«$mo_moOoZ S>mB© `wº$ eH©$am (gwH«$moµO) Ho$ _mÜ`_ _| ßboQ> {H$`m J`m & naÝVw H$moB©
?

(I) ? 2

18. _ÀN>a à{VH$fu ([anobÝQ>) {H$g àH$ma H$m`© H$aVo h¢ ? BgHo$ à_wI amgm`{ZH$ g§KQ>H$m| H$m
& 2

19. _mZd OrZmo_ _| bJ^J 1.6 {_{b`Z go 3.2 {_{b`Z {dñWb h¢, Ohm± SNPs nmE Om
gH$Vo h¢, naÝVw do OrZ Ho$ àH$m`© H$mo à^m{dV H$a ^r gH$Vo h¢ Am¡a Zht ^r & g_w{MV
H$maUm| H$s ghm`Vm go H$WZ H$s Ý`m`g§JVVm {gÕ H$s{OE & 2

20. (H$) àmW{_H$ H$mo{eH$m g§dY© VWm {ÛVr`H$ H$mo{eH$m g§dY© Ho$ ~rM {d^oX H$s{OE & 2

AWdm
(I) gr{_V H$mo{eH$m d§e (bmBÝg) VWm gVV H$mo{eH$m d§e _| A§Va ñnï H$s{OE & 2

21. EH$ à{VO¡{dH$ H$m {Z_m©U H$aZo dmbo gyú_Ord H$s à`moJembm _| n¥W H$Z (ñH«$sqZJ) H¡$go
H$a|Jo ? 2

IÊS> J

22. {ZåZ{b{IV H$m H$maU {b{IE : 3

(H$) ~¡\$b âbmñH$ _| V-AmH$ma H$m Im±Mm hmoVm h¡ &
(I) gyú_O¡{dH$ àH$m`m] _| PmJ H$s A{YH$Vm EH$ g_ñ`m h¡ &
(J)
{bE {H$`m OmVm h¡ &

99 Page 10 of 19

SECTION B

17. (a) For selection of recombinants having lacZ gene on vector DNA, it
was plated on medium containing sucrose with a chromogen dye.
(Show sucrose breakdown) No result was obtained. Why ?

(b) What can be done to select these recombinants ? 2

18. How does mosquito repellant work ? Mention its major chemical
constituents. 2

19. 6 million to 3 2 million sites in the human genome,
where SNPs can occur, but still they may or may not affect gene
ustify the statement giving reasons. 2

20. (a) Differentiate between Primary cell cultures and Secondary cell
cultures. 2

OR

(b) Differentiate between Finite cell lines and Continuous cell lines. 2

21. How will you perform laboratory screening for micro-organism producing
an antibiotic ? 2

SECTION C

22. Give reason for the following : 3

(a) Baffle flasks have V-shaped notch.

(b) Excess foaming in microbiological processes is a problem.

(c) Slaughter-house wastes are used to prepare media for
microbiological processes.

99 Page 11 of 19 P.T.O.

23. (H$) Myhm| Ho$ _m°S>b (à{Vê$n) H¡$go ~ZmE OmVo h¢ ? _yb H$mo{eH$m (ñQ>o_ gob) àm¡Úmo{JH$s
Ho$ joÌ _| Xmo à_wI Cn`moJ {b{IE & 3

AWdm
(I) OrZ Zm°H$AmCQ> ~ZmZo Ho$ {bE {ZåZ àdmh AmaoI _| A, B VWm C H$mo C{MV eãX
Ûmam ^[aE & 3

Zm°H$AmCQ> Ho$ EH$ dm§{N>V OrZ H$m M`Z

ßb¡pµÁ_S>> _| aIZm

A _| ßb¡pµÁ_S>> H$m {ZdoeZ

g_OmVr` nwZ`m}OZ

B Ho$ gmW M`Z

C _| {Zdo{eV H$aZm

23

(I) BLAST ? Bg A§dofU H$m`©H«$_ _| Amdo{ï>V ? 3

24. gwHo$pÝÐH$s`m| Ho$ OrZ H$s A{^ì`{º$ Ho$ {bE gdm}n`wº$ nanmofr h¡ & VrZ
g_w{MV H$maUm| Ûmam H$WZ H$m g_W©Z H$s{OE & 3

25. nmaOrdr µ\$gbm|
µ\ ? 3

99 Page 12 of 19

23. (a) How are mouse models created ? Write two major applications in
the field of stem cell technology. 3

OR
(b) Complete the flowchart for creating gene knockout by filling A, B
and C. 3

Selection of a gene of interest for knockout

Place in plasmid

Inject plasmid into A

Homologous recombination

Selection with B

Injecting it into C

(For visually-impaired candidates only in lieu of Q. 23 (b))

(b) What is BLAST ? What are the principles involved in this search
program ? 3

24. Pichia pastoris is the most suitable host to express eukaryotic gene.
Support the statement giving three reasons. 3

25. Transgenic crops are facing challenges globally. What are the major
concerns about these GM crops ? 3

99 Page 13 of 19 P.T.O.

26. (H$) ?

(I) {H$gr ^r H$mo{eH$m _| àmoQ>r`mo_ J{VO h¢ & ?

(J) {H$gr H$mo{eH$m _| m-RNA VWm àmoQ>rZ H$s A{^ì`{º$ gXm ghg§~§Yr Zht hmoVo &
? 1+1+1

27. noßQ>mBS>m| VWm àmoQ>rZm| Ho$ dmînrH$aU VWm àmoQ>m°ZZ àH«$_ _| MALDI (_¡
boOa {S>gmon©eZ Am`ZmBµOoeZ) VH$ZrH$ H$m Cn`moJ H¡$go H$aVo h¢ ? 3

28. (H$) EH$ g§dmhH$ _| H$m¡Z-go A{Zdm`© A{^bjU hmoZo Mm{hE ?
(I) eQ>b g§dmhH$ ? 2+1

IÊS> K

29. (H$) H$a AJ«Jm_r àíZm| Ho$ CÎma {b{IE :
EH$b (SNPs) ì`{º$`m| _| nmB© OmZo dmbr gm_mÝ`
AmZwd§{eH$ {d{^ÞVmE± h¢ & àË`oH$ SNP moQ>mBS> _| {d{^ÞVm H$mo
{Zê${nV H$aVm h¡ VWm {H$gr ^r ì`{º$ Ho$ S>r.EZ.E. _| gm_mÝ` ê$n go nm`m OmVm
h¡ & SNP {d{^Þ amoJm| go g§~Õ OrZm| H$mo Ty> Zo _| ghm`Vm H$aVo h¢ & H¢$ga VWm
_Yw_oh O¡go amoJm| Ho$ {bE CÎmaXm`r OrZm| H$s nhMmZ H$aZo Ho$ {bE emoY hmo ahm h¡ &
(i) {M{H$ËgH$m| Ho$ {bE SNPs {H$g àH$ma ghm`H$ hmo gH$Vo h¢ ? 1

(ii) g^r àH$ma Ho$ amoJm| go h_mar g§doXZerbVm VWm à{VajU H$s nhMmZ
SNPs H$a gH$Vo h¢ & BgHo$ g_W©Z _| EH$ CXmhaU Xr{OE & 2

(iii) g_{ï> AmZwd§{eH$s _| SNP ? 1

AWdm
(iii) SNPs Ho$db _mZd OrZmo_ Ho$ H$moqS>J joÌm| _| hr nmE OmVo h¢ AWdm
H$moqS>J VWm Zm°Z H$moqS>J ñWbm| XmoZm| _| nmE OmVo h¢ ? 1

99 Page 14 of 19

26. (a) Among all the biomolecules, proteins have maximum diversity in
function. Why ?

(b) Proteome of a given cell is dynamic. Why ?

(c) m-RNA and protein expression levels in a cell do not always
correlate. Why ? 1+1+1

27. How is MALDI (Matrix Assisted Laser Desorption Ionisation) used to
volatalise and protonate peptides and proteins ? 3

28. (a) What are the essential features that a vector should possess ?

(b) What are shuttle vectors ? 2+1

SECTION D

29. (a) Read the passage and answer the question that follows :
Single nucleotide polymorphisms (SNPs) are common genetic
variations among people. Each SNP represents a difference in a

SNPs can help scientists to locate genes associated with disease.
Research is going on to identify SNPs associated with diseases like
diabetes, cancer etc.

(i) How can SNPs help physicians ? 1

(ii) SNPs can decide our susceptibility to, or protection from all
kinds of diseases. Give any one example to support the same. 2

(iii) Why is SNP analysis used in population genetics ? 1

OR

(iii) Do SNPs occur only in coding regions of human genome or in
both coding and non-coding regions ? 1

99 Page 15 of 19 P.T.O.

30. (H$) àmUr H$mo{eH$m g§dY©Z
àmUr H$mo{eH$mAm| H$m g§dY©Z H$m±M AWdm ßbmpñQ>H$ Ho$ nmÌm| _| g§dY©Z _mÜ`_ _|
{H$`m OmVm h¡ & {ZpíMV g_`m§Vamb Ho$ ~mX g§dY©Z _mÜ`_ H$mo ~Xbm OmVm h¡ &
Am¡a BgHo$ AmJo Zht & d¥{Õ H$aVo
g_`, H$mo{eH$mE± g§ñne© g§X_Z Xem©Vr h¢ naÝVw A~w©X (H¢$ga) H$s H$mo{eH$mE± Bg
A{^bjU H$mo Zht Xem©Vr h¢ &
(i) ? 1

(ii) H$moB© H$mo{eH$m àgm_mÝ` h¡ AWdm H¢$gaOZr `h kmV H$aZo Ho$ {bE A~w©X
{deofk H$mo{eH$mAm| Ho$ {H$g A{^bjU H$m AÜ``Z H$aVo h¢ ? 1
(iii) n`m©daU nmÌo n`m©daU go {^Þ h¡, `h g§d{Y©V H$mo{eH$mAm|
H$mo {H$g àH$ma à^m{dV H$aVm h¡ ? {H$Ýht 2

AWdm
(iii) gm_mÝ` Ord H$s g^r àgm_mÝ` àmUr H$mo{eH$mE± Zída h¢ & H$maU g{hV
H$WZ H$s Ý`m`g§JVVm H$s nw{ï> H$s{OE & 2

IÊS> L>

31. (H$) (i) àmW{_H$ VWm {ÛVr`H$ CnmnM`O _| {d^oX H$s{OE &
(ii) H$mo{eH$m VWm D$VH$ g§dY©Z Ûmam CËnm{XV VrZ _hÎdnyU© Am¡Úmo{JH$ {ÛVr`H$
nmXn CnmnM`Om| Ho$ Zm_ {bIH$a C & 2+3

AWdm
(I) (i) {díd ñdmñÏ` g§JR>Z (WHO) Ho$ AZw_mZ Ho$ AZwgma, bJ^J 228 {_{b`Z
A H$s H$_r go à^m{dV h¢ & AmZwd§{eH$ A{^`m§{ÌH$s
(B§Or{Z`[a¨J) Zo Bg g_ñ`m H$m g_mYmZ H¡$go {dH${gV {H$`m ?
(ii) ?
(iii) Eogo Xmo A{Zdm`© Eo_rZmo Aåb Ho$ Zm_ {b{IE {OZH$s XbhZ _| H$_r
(A^md) h¡ & 3+1+1

99 Page 16 of 19

30. (a) Animal cell culture

Animal cells can be grown in glass or plastic vessels with nutrient
medium. These need to be given fresh medium periodically. The
infant animal cells grow only to adulthood and not any further.
While growing, they show phenomenon of contact inhibition, but

(i) What is contact inhibition ? 1

(ii) Which property of cells is studied by oncologists to determine
whether cells are normal or cancerous ? 1

(iii) As in vivo environment is different from in vitro environment,
how does it affect cells grown in culture ? Mention any two. 2

OR
(iii) Mortality is associated with all normal animal cells. Justify
giving reason. 2

SECTION E

31. (a) (i) Differentiate between primary and secondary metabolites.
(ii) Name three industrially important plant secondary
metabolites produced through cell and tissue culture, along
with their use. 2+3

OR

(b) (i) According to WHO estimates, approximately 228 million
children are affected due to Vitamin A deficiency. How has
genetic engineering provided/developed a solution to the
same ? Explain.
(ii) Why is nutritional quality of cereals and legumes limited ?
(iii) Name two essential amino acids which are deficient in pulses.
3+1+1

99 Page 17 of 19 P.T.O.

32. (H$) (i) nwZ`m}JO S>r.EZ.E. àm¡Úmo{JH$s Ho$ _w»` MaUm| H$m `moOZmË_H$ àdmh AmaoI
~ZmBE & 4

32 (i)
(i) nwZ`m}JO S>r.EZ.E. àm¡Úmo{JH$s Ho$ g^r Mma à_wI MaUm| H$mo ghr H«$_ _|
{b{IE & Eogo {H$Ýht Xmo d¡km{ZH$m| Ho$ Zm_ {b{IE {OÝhm|Zo nwZ`m}JO
S>r.EZ.E. AUw H$m gd©àW_ {Z_m©U {H$`m & Bg àm¡Úmo{JH$s Ho$ Cn`moJ Ûmam
{Z{_©V Xmo ì`mdgm{`H$ CËnmXm| Ho$ Zm_ {b{IE & 2+1+1
(ii) Cn`w©º$ àm¡Úmo{JH$s _| Q>mBn II à{V~§YZ E§µOm
Xr OmVr h¡ ? 1

AWdm
(I) (i) Cg VH$ZrH$ H$m dU©Z H$s{OE {Ogo n`m©daUr` Z_yZm| _| ~hþV H$_ (Aën)
g§»`m _| CnpñWV gyú_Ordm| H$s CnpñW{V H$m nVm bJmZo Ho$ {bE BñVo_mb
{H$`m OmVm h¡ &
(ii) ? 3+2

33. (H$) (i) ?

(ii) EnrQ>moßg (A{ ?
(iii) ? 2+1+2

AWdm
(I) (i) noer` àmoQ>rZm| Ho$ O¡d-g§íbofU Ho$ {bE emImpÝdV ûm¥§Ibm Eo_rZmo Aåb
(BCAA) ? ì`m»`m H$s{OE &
(ii) àmoQ>rZ H$m O¡d ?
(iii) ? {ZåZ{b{IV àmoQ>rZm| H$mo CZHo$ àmoQ>rZ XjVm
AZwnmV Ho$ KQ>Vo H«$_ (õmg_mZ) _| bJmBE : 3+1+1
XÿY, Ho$grZ, gmo`m, Johÿ±, Xhr H$m nmZr

99 Page 18 of 19

32. (a) (i) Draw diagram showing schematic representation of the basic
steps in Recombinant DNA Technology. 4

(For visually-Impaired candidates only in lieu of
Q. 32 (a) (i))

(i) Write all the four defined steps of Recombinant DNA
Technology in correct sequence. Name any two scientists who
were involved in generation of first Recombinant DNA
molecule. Name two products produced commercially using
this technology. 2+1+1
(ii) Why are Type II restriction enzymes preferred in the above
mentioned technology ? 1

OR
(b) (i) Describe the technique to detect microbes from environmental
samples present in very small numbers.

(ii) Why is this technique getting popular in diagnostics ? 3+2

33. (a) (i) How are conventional vaccines different from recombinant
vaccines ?
(ii) What are epitopes ?
(iii) What are the advantages of preparing vaccines using
epitopes ? 2+1+2

OR
(b) (i) Branched Chain Amino acids (BCAA) are essential for the
biosynthesis of muscle protein. How ? Explain.

(ii) What is Biological value of proteins ?
(iii) What is Protein Efficiency Ratio ? Arrange the following
proteins in decreasing order of protein efficiency ratio : 3+1+1
Milk, Casein, Soya, Wheat, Whey

99 Page 19 of 19 P.T.O.