AHSEC 2nd Year Syllabus 2024-25 Biotechnology

SYLLABUS FOR HIGHER SECONDARY COURSE
Biotechnology, in its broadest sense, is the technology that provides goods and services by industrial
processes using biological organisms, systems and processes. It comprises a number of technologies based
upon increasing understanding of biology at the cellular and molecular level. The techniques of biotechnology
includes recombinant DNA technology (genetic engineering), hybridoma technology and monoclonal anti-
body preparation, cell and tissue culture, DNA fingerprinting, protoplast fusion, protein engineering, immobi-
lized enzyme technology, cell catalysis, biosensor and several others. Biotechnology has emerged as one of
the frontline technologies in recent times. Biotechnology with its most recent offshoot Bioinformatics is being
projected as the technology that would have the greatest impact in the coming years worldwide.
With the exponential growth of human population, it becomes urgent to improve the production pro-
cess and capabilities for the increased production of food, fuel, medicine, enzymes, fermented items, fibers,
vaccines and biofertilizers. It also becomes important to ensure protection, conservation and sustainable
utilization of our natural resources. Biotechnology has the answer for these problems. Application of biotech-
nology has been proved to be fruitful for meeting the need of the modern human society,
Inclusion of Biotechnology in higher secondary level courses is considered as important to create a
base and interest among the students for higher education, training and research in Biotechnology. In view of
this the present syllabus is designed to cater needs of the Biotechnology education for the higher secondary
students of Assam. The theoretical topics and experiments are selected and organized such a way so that the
students can earn basic concept and interlink the various topics and techniques. It is expected that the student
will gain appropriate knowledge and acquire practical skill on the subject. It is also anticipated that the course
will make the students competent to meet up the challenges of both academic and professional courses
beyond the secondary level.
Objectives :
The objectives of teaching Biotechnology at Higher Secondary level are :
1. To create an interest among the students of H.S. Classes to study Biotechnology courses.
2. To help the students to know and acquire basic information and concept in the subject.
3. To expose the students to understand the basic techniques and their utilization in various produc-
tion and service industries.
4. To familiarize the learners to understand the importance and applications of Biotechnology in
everyday life.
5. To develop conceptual competence of the students so as to cope-up with technical and profes-
sional in future carrier.

2 Syllabi for H.S. Final Year

SYLLABUS FOR HIGHER SECONDARY FINAL YEAR COURSE
One Paper Time : Three Hours Total Marks-70
Pass Marks-21
Unitwise Distribution of Marks and Periods :
Unit No. Title Marks Periods
Unit-1 Protein Engineering and Bioinformatics 15 23
Unit-2 Genetic Engineering and genomics 15 32
Unit-3 Environmental Biotechnology & Bioethics 10 20
Unit-4 Microbial Technology 10 25
Unit-5 Plant Cell Culture Technology 10 25
Unit-6 Animal Cell Culture Technology 10 25
Total 70 150
Unitwise Distribution of Course contents : Marks
Unit-1 : Protein Engineering and Bioinformatics : 15
Protein based products and designing
Proteins
Proteomics : an introduction
Introduction to Bioinformatics
Sequences and Nomenclature
Information Sources
Analysis using Bioformatics tools
Unit-2 : Genetic Engineering and Genomics : 15
Recombinant DNA technology-definition and tools
Making recombinant DNA
Construction of DNA library :
Genomic and CDNA
Cloning vectors
Polymerase Chain Reaction (PCR)
DNA probes
Hybridization techniques : Southern, Northern and Western
DNA sequencing
Genomics : an introduction
Unit-3 : Environmental Biotechnology & Bioethics : 10
Bioremediation of oil pollution reducing environmental impact of chemical
herbicides & fertilizers; biosensors to detect environmental pollution.

Syllabi for H.S. Final Year 3

Biofertilizers-definition and uses.
Biofuels : definition and application
Genetically Modified Organisms and
Ethical Issue
Intellectual Property Rights-Patenting Life forms
Unit-IV : Microbial Technology 10
Classification of microorganism
Microbial culture techniques
Measurement and kinetics of microbial growth
Strain Isolation and Isolation of microbial products
Application of microbial culture
Unit-V : Plant cell culture Technology 10
Introduction; Cellular Toti potency
Plant cell and tissue culture techniques and media
Application of plant tissue culture
Gene transfer methods in plants
Transgenic plants for crop improvement
Unit-VI : Animal Cell Culture Technology 10
Introduction
Animal Cell Culture Technology and media
Characterization of cell lines
Scale up of animal cell culture process
Application of animal cell culture
Stem cell technology
SYLLABUS FOR BIOTECHNOLOGY PRACTICAL
Total Marks-30
Scheme of Evolution : Marks
1. Two Experiments 8 + 8 = 16
(One computer based Practical)
™ Data retrieval and data search using Internet site of NCBI
™ Download a DNA protein sequence from Internet, analyze and comment over it
™ Ion-exchange chromatography for protein
™ Estimation of DNA
™ Isolation of microbes from a given biological sample
™ Sterilization techniques : Dry heat and moist heat sterilization,
Chemical sterilization and ultra filtration
™ Determination of bacterial growth curve

4 Syllabi for H.S. Final Year

™ Determination of blood groups
™ Estimation of blood glucose by enzymatic method
™ Demonstration of plant tissue culture technique
™ Isolation of bacterial plasmid DNA and its detection by gel electrophoresis
™ Minor project work/Seminar
2. Viva on practical 4
3. Practical Record 4
4. Seminar/Minor project 6
Recommended Books
1. CBSE publication for class XI and XII
ANNEXURE
Laboratory Requirements :
A. Must include the following components for Laboratory:
(a) One small lab with Laminar Air flow cabinete and Single working desk
(b) Working Laboratory with working table & Chairs, Washing facilities, light arrangement.
(c) Essential equipments : Autoclave, oven, Refrigerators, Incubator, Water distillation, Centrifuge.
(d) Glassware’s, measuring equipment etc.
(e) Small Culture room (air conditioned) and culture racks.

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