CBSE Class 12 Biotechnology Question Paper 2019

SET-4
Series BVM H$moS> Z§.
Code No. 99
amob Z§. narjmWu H$moS >H$mo CÎma-nwpñVH$m Ho$ _wI-n¥ð
Roll No. >na Adí` {bIo§ &
Candidates must write the Code on the
title page of the answer-book.

 H¥$n`m Om±M H$a b| {H$ Bg àíZ-nÌ _o§ _w{ÐV n¥ð> 8 h¢ &
 àíZ-nÌ _| Xm{hZo hmW H$s Amoa {XE JE H$moS >Zå~a H$mo N>mÌ CÎma -nwpñVH$m Ho$ _wI-n¥ð> na
{bI| &
 H¥$n`m Om±M H$a b| {H$ Bg àíZ-nÌ _| >28 àíZ h¢ &
 H¥$n`m àíZ H$m CÎma {bIZm ewê$ H$aZo go nhbo, àíZ H$m H«$_m§H$ Adí` {bI| &
 Bg àíZ-nÌ H$mo n‹T>Zo Ho$ {bE 15 {_ZQ >H$m g_` {X`m J`m h¡ & àíZ-nÌ H$m {dVaU nydm©•
_| 10.15 ~Oo {H$`m OmEJm & 10.15 ~Oo go 10.30 ~Oo VH$ N>mÌ Ho$db àíZ-nÌ H$mo n‹T>|Jo
Am¡a Bg Ad{Y Ho$ Xm¡amZ do CÎma-nwpñVH$m na H$moB© CÎma Zht {bI|Jo &
 Please check that this question paper contains 8 printed pages.
 Code number given on the right hand side of the question paper should be
written on the title page of the answer-book by the candidate.
 Please check that this question paper contains 28 questions.
 Please write down the Serial Number of the question before
attempting it.
 15 minute time has been allotted to read this question paper. The question
paper will be distributed at 10.15 a.m. From 10.15 a.m. to 10.30 a.m., the
students will read the question paper only and will not write any answer on
the answer-book during this period.

O¡d-àm¡Úmo{JH$s
BIOTECHNOLOGY
{ZYm©[aV g_` : 3 KÊQ>o A{YH$V_ A§H$ : 70
Time allowed : 3 hours Maximum Marks : 70

99 1 P.T.O.

gm_mÝ` {ZX}e :
(i) àíZ-nÌ _| Mma IÊS> H$, I, J Am¡a K h¢ &
(ii) g^r IÊS>m| Ho$ Hw$N> àíZm| _| Am§V[aH$ M`Z {dH$ën h¢ & Eogo àíZm| _| AmnH$mo Cg àíZ Ho$ Ho$db
EH$ {dH$ën H$m hr CÎma XoZm h¡ &
(iii) àíZ g§»`m 1 go 6 VH$ Ho$ àíZ A{VbKwÎmamË_H$ àíZ h¢, {OZ_| go àË`oH$ H$m EH$-EH$ A§H$ h¡ &
(iv) àíZ g§»`m 7 go 14 VH$ Ho$ àíZ bKwÎmamË_H$ h¢, {OZ_| go àË`oH$ Ho$ Xmo-Xmo A§H$ h¢ &
(v) àíZ g§»`m 15 go 25 VH$ Ho$ àíZ ^r bKwÎmamË_H$ h¢, {OZ_| go àË`oH$ Ho$ VrZ-VrZ A§H$ h¢ &
(vi) àíZ g§»`m 26 go 28 VH$ Ho$ àíZ XrK© CÎmamË_H$ h¢, {OZ_| go àË`oH$ Ho$ nm±M-nm±M A§H$ h¢ &
(vii) H¡$ëHw$boQ>am| (g§JUH$m|) H$m Cn`moJ d{O©V h¡ & {\$a ^r, `{X Amdí`H$ hmo, Vmo Amn bm°J-
gma{U`m| H$m Cn`moJ H$a gH$Vo h¢ &
General Instructions :
(i) Question paper contains four sections A, B, C and D.
(ii) There is internal choice in questions in all the sections. You have to attempt only
one of the choices in such questions.
(iii) Questions No. 1 to 6 are very short answer questions, carrying 1 mark each.
(iv) Questions No. 7 to 14 are short answer questions, carrying 2 marks each.
(v) Questions No. 15 to 25 are also short answer questions, carrying 3 marks each.
(vi) Questions No. 26 to 28 are long answer questions, carrying 5 marks each.
(vii) Use of calculators is not permitted. However, you may use log tables, if necessary.

IÊS> H$
SECTION A

1. {dbmo_ Q´>m§g{H«$ßQ>oµO E§µOmB_ H$m Š`m H$m`© h¡ ? 1
{H$gr g§dmhH$ _| ~hþghb½ZH$m| H$mo Cn`moJ H$aZo H$m Š`m bm^ h¡ ? 1
What is the function of reverse transcriptase enzyme ?
OR
What is the advantage of using polylinker in a vector ?

99 2

2. nmXn D$VH$ go H$mo{eH$m {Zb§~Z g§dY© àmá H$aZo Ho$ {bE _¡{gamoµOmB_ H$m Cn`moJ Š`m|
{H$`m OmVm h¡ ? 1
\$bm| Ho$ nH$Zo go gå~pÝYV hm°_m}Z H$m Zm_ {b{IE & 1
Why is macerozyme used in obtaining cell suspension culture from plant
tissue ?
OR
Name the hormone involved in fruit ripening.
3. IUPAC EH$b dU© Hy$Q> ‘R’ H$m Š`m AW© h¡ ? 1
What does IUPAC single letter code ‘R’ stand for ?
4. bmBnmo\o$ŠeZ Š`m h¡ ? 1
What is lipofection ?
5. `{X {H$gr {db{JV OrdmUw à^oX H$s {d{eîQ> d¥{Õ Amdí`H$VmE± kmV Z hm|, Vmo BgHo$
g§dY©Z Ho$ {bE {H$g àH$ma Ho$ _mÜ`_ H$m Cn`moJ H$aZm Mm{hE ? 1
If the specific growth requirements of an isolated strain of bacterium are
not known, which type of medium should be used to grow (culture) the
same ?
6. O¡d-AUwAm| _| Xþ~©b AmH$f©U ~b Ûmam {H$g àH$ma H$m A-ghg§`moOr nmañn[aH$ Am~§Y
_hÎdnyÊm© h¡ ? 1
Which type of non-covalent interaction, through a weak attraction force,
is important in biomolecules ?
IÊS> I
SECTION B

7. _Zwî`m| Ho$ {bE ‘Zd© J¡g’ {H$g àH$ma KmVH$ hmoVr h¡ ? 2
How does nerve gas cause death in humans ?

8. àË`oH$ Ho$ EH$ CXmhaU H$s ghm`Vm go nm¡Ym| _| àmW{_H$ Ed§ {ÛVr`H$ CnmnM`Om| _| {d^oX
H$s{OE & 2
A§Vamd§er` g§H$aU go g§H$[aV-Ordm| H$mo àmá H$aZo _| g\$bVm {_bZm AË`§V H${R>Z h¡ &
Š`m| ? 2
Differentiate between primary and secondary metabolites in plants, with
one example of each.
OR
Inter-generic crosses hardly lead to successful production of hybrids. Why ?

99 3 P.T.O.

9. AmZwd§{eH$ A{^`m§{ÌH$s VH$ZrH$m| H$m Cn`moJ H$aHo$ ‘JmoëS>Z amBg (Mmdb)’, EH$ nmaOrZr
(Q´>m§gOo{ZH$) nmXn, H$m {dH$mg H¡$go {H$`m J`m ? 2
How was ‘Golden Rice’, a transgenic plant, developed using genetic
engineering techniques ?

10. OKT-3 à{Vamon AñdrH$aU H$mo {H$g àH$ma amoH$Zo _| ghm`Vm H$a gH$Vm h¡ ? 2

tPA öX`mKmV H$m {ZamoY {H$g àH$ma H$a gH$Vm h¡ ? 2
OKT-3 can help in preventing transplant rejection. How ?

OR

How can tPA prevent heart attack ?

11. g§dY© _§o ñVZYm[a`m| H$s H$mo{eH$mAm| H$s CÎmaOr{dVm Ho$ {bE ~mø-H$mo{eH$s` Ed§
A§Vam-H$mo{eH$s` pH AZwajU Amdí`H$ h¡ & Š`m| ? 2
Maintenance of extra-cellular and intra-cellular pH is essential for
survival of mammalian cells in culture. Why ?

12. AmnH$mo {H$gr bo~b Ho$ ~J¡a EH$ AZwH«$_ {X`m J`m h¡ & Amn {H$g àH$ma nhMmZ|Jo {H$ dh
DNA AWdm RNA h¡ AWdm àmoQ>rZ AZwH«$_ I§S> h¡ ? 2
You are given a sequence without label. How will you find out if it is a
DNA or RNA or a protein sequence ?

13. EH$ àmoQ>rZ H$m AUw ^ma 20,000 D h¡, {Og_| 5 Am¡a 4 Amdoe h¡ & g§h{V-ñnoŠQ´>mo_rQ>a
{H$g m/z na àmoQ>rZ Am`Z H$mo nhMmZ gHo$Jm ? 2
A protein with molecular weight of 20,000 D contains 5 and 4 charges. At
what m/z are protein ions detected by the mass spectrometer ?

99 4

14. {XE JE OrZmo_ AZwH«$_ _|, OrZm| H$s g§»`m VWm àmoQ>rZm| H$s g§»`m _| a¡{IH$ g§~§Y Zht h¡ &
Š`m| ? Xmo H$maU ~VmBE & 2
àH$m`m©Ë_H$ OrZmo{_Šg _| S>r.EZ.E. {Mn {H$g àH$ma Cn`moJr h¢ ? 2
In a given genome sequence, the relationship between number of genes
and number of proteins is not linear. Why ? Give two reasons.
OR
How are DNA chips useful in functional genomics ?

IÊS> J
SECTION C

15. AmOH$b {S>Q>OªoQ (An_mO©H$) _| ‘O¡{dH$ g{H«$` E§µOmB_’ hmoVo h¢ &> 3
(H$) gm_mÝ` ê$n go Cn`moJ {H$E OmZo dmbo E§µOmB_ H$m Zm_ {b{IE &
(I) {da§OH$ H$s CnpñW{V _| `h E§µOmB_ {ZpîH«$` Š`m| hmo OmVm h¡ ?
(J) AmZwd§{eH$V: {Z{_©V E§µOmB_ àmH¥${VH$ E§µOmB_ go {H$g àH$ma {^Þ h¡ ?
Detergents now-a-days are provided with ‘biologically active enzymes’.
(a) Name the enzyme commonly used.
(b) Why is this enzyme inactivated in the presence of bleach ?
(c) How is the engineered enzyme different from its natural form ?

16. EH$ àdmh MmQ>© Ûmam B©.H$mobmB© _| {Z{_©V nwZ`m©oJO BÝgw{bZ (øy_{y bZ) Ho$ n¥WŠH$aU Ho$
{d{^Þ MaUm| H$mo Xem©BE & 3
gwajm hoVw gyú_Ordr O¡d-àm¡Úmo{JH$s go gå~pÝYV à_wI qMVmE± Š`m-Š`m h¢ ? 3
Draw a flow chart to show the various steps used for the isolation of
recombinant insulin (Humulin) produced in E. coli.
OR
What are the main concerns for the safety aspects related to microbial
biotechnology ?

17. _oQ>m OrZmo{_Šg Ho$ Cn`moJ Ûmam d¡km{ZH$ n`m©daU _| CnpñWV ZdrZ OrZ CËnmXm| H$s nhMmZ
{H$g àH$ma H$a gH$Vo h¢ ? 3
How does meta genomics approach help scientists to identify the novel
gene products present in the environment ?

99 5 P.T.O.

18. ~¡M VWm gVV g§dY© Ho$ ~rM A§Va ñnîQ> H$s{OE & 3
(H$) {H$ÊdZ-g§`§Ìm| (\$_]Q>am|) _| ~mYH$ (~¡\$ëg) Š`m| CnbãY H$amE OmVo h¢ ?
(I) g§dY©Z _mÜ`_ H$s \$moq_J gyú_Ordm| H$s d¥{Õ _| AdamoY CËnÞ H$a gH$Vr h¡ &
Š`m| ?
(J) gyú_Ordr à^oXm| H$s gm_mÝ`V: ZmBQ´>mogmo½dm{ZS>rZ (NTG) O¡go agm`Zm| go AZmd¥V
{H$`m OmVm h¡ & Š`m| ? 3
Differentiate between Batch and Continuous culture.
OR
(a) Why are fermentors provided with baffles ?
(b) Foaming of (in) medium can hinder microbial growth. Why ?
(c) Microbial strains are usually exposed to chemicals like NTG
(Nitrosoguanidine). Why ?

19. àmoQ>rZm| Ho$ n¥WŠH$aU Ho$ {bE Obr` {ÛàmdñWm {d^mOZ à{H«$`m H$m dU©Z H$s{OE & 3
Describe aqueous two-phase partition process for separation of proteins.

20. EH$ CXmhaU H$s ghm`Vm go {ZåZ{b{IV H$mo g_PmBE :
(H$) H§w$X {gao dmbo H$V©H$ à{V~§YZ E§µOmB_
(I) {Mn{Mno {gao dmbo H$V©H$ à{V~§YZ E§µOmB_
{XemË_H$ OrZ ŠbmoqZJ Ho$ {bE H$m¡Z-go {gao ~ohVa hmoVo h¢ Am¡a Š`m| ? 3
With an example, show
(a) blunt-end cutter restriction enzyme.
(b) sticky-end cutter restriction enzyme.
Which one of the ends are better for directional gene cloning and why ?

21. Zm_m§{H$V AmaoI H$s ghm`Vm go {H$gr S>r.EZ.E. (DNA) Oob Ho$ {d{eîQ> AZwH«$_ H$mo
EfUr (àmo~) H$s ghm`Vm go Ty>±‹T>Zo Am¡a nhMmZZo Ho$ {bE à`wº$ hmoZo dmbr AÝdofr VH$ZrH$
àX{e©V H$s{OE & 3
AmaoI H$s ghm`Vm go pBR 322 VWm YEp Ho$ bjUm| H$s VwbZm H$s{OE & YEp H$mo
eQ>b g§dmhH$ Š`m| H$hVo h¢ ? 3
Draw a labelled diagram showing the technique which can be used to
identify and locate a specific sequence in a DNA gel using a probe.
OR
Draw a diagram to show and compare the features of pBR 322 and YEp .
Why is YEp called a shuttle vector ?

99 6

22. Eogo {H$Ýht VrZ VarH$m| H$m dU©Z H$s{OE {OZgo nanmofr H$mo{eH$mAm| _| nwZ`m}JO S>r.EZ.E.
H$m {ZdoeZ H$am`m Om gH$Vm h¡ & 3
Describe any three methods used to introduce recombinant DNA into the
host cells.

23. {ZåZ{b{IV nXm| H$s n[a^mfm {b{IE : Ref-AZwH«$_ (RefSeq), g_OmV (hmo_mobmoJ) Am¡a
VH$m©^mgr (n¡ambm°J) 3
BLAST Š`m h¡ ? BLAST ImoO VH$ZrH$ {H$Z {gÕmÝVm| na AmYm[aV h¡, {b{IE & 3
Define the terms : RefSeq, Homologues and Paralogs
OR
What is BLAST ? Write the principles that underlie BLAST search.

24. O¡dgyMZm {dkmZ (~m`moBÝ\$m°_}{Q>Šg) _| Cn`moJ {H$E OmZo dmbo H$moB© VrZ S>oQ>m~og Ho$ Zm_
{b{IE Am¡a CZ_| {Z{hV gyMZm H$m ^r CëboI H$s{OE & 3
Name any three databases for Bioinformatics and the information
available in them.

25. {H$gr {XE JE OrZmo_ _| `{X h_| OrZm| H$s AdpñW{V kmV ^r hmo, Vmo ^r CZH$s JUZm
H$aZm _wpíH$b H$m`© Š`m| h¡ ? H$moB© VrZ H$maU {b{IE & 3
Why are genes difficult to count, even if we know where the genes are in
a given genome ? Write any three reasons.

IÊS> K
SECTION D

26. (H$) ñQ>o_ H$mo{eH$mE± {H$Ýh| H$hVo h¢ ?
(I) ~hþeº$ (ßbyarnmoQ>|Q>) Am¡a ~hþj_ (_ëQ>rnmoQ>|Q>) ñQ>o_ H$mo{eH$mAm| _| {d^oX H$s{OE &
BZ_| go H$m¡Z-gr eara H$s _aå_V _| BñVo_mb hmoVr h¢ Am¡a H¡$go ?
(J) ñQ>o_ H$mo{eH$mAm| H$s {M{H$Ëgm joÌ _| VrZ AZwà`moJ ~VmBE & 5

(H$) "OrZ {ZagZ' Š`m h¡ ?
(I) OrZ {ZagZ go V¡`ma Myhm| Ho$ Z_yZo {H$g àH$ma Cn`moJr h¢ ?
(J) {H$g d¡km{ZH$ Zo _mZd ^y«Ur` _yb H$mo{eH$mAm| (hESC) Ho$ g§dY©Z H$aZo H$s
VH$ZrH$ {dH${gV H$s Am¡a BZ H$mo{eH$mAm| H$m òmoV Š`m h¡ ? 5

99 7 P.T.O.

(a) What are stem cells ?
(b) Differentiate between pluripotent and multipotent stem cells.
Which of these act as repair system for the body and how ?
(c) Give three applications of stem cells in the field of medicine.
OR
(a) What is a ‘gene knockout’ ?
(b) Why are mouse models prepared using gene knockouts useful ?
(c) Who developed the technique to grow Human Embryonic Stem
Cells (hESCs) in culture and what is the source of these cells ?

27. g_PmBE {H$ nmXnm| _| doŠQ>a _Ü`ñW OrZ ñWmZmÝVaU H$m à`moJ H$aVo hþE dm±{N>V OrZ {H$g
àH$ma {Zdo{eV {H$E OmVo h¢ & AmaoI ~ZmBE & Q´>m§gOo{ZH$ nmXn Am¡a µJ¡a-Q´>m§gOo{ZH$ nmXn _|
h_ H¡$go {d^oX H$a gH$Vo h¢ ? 5
(H$) ImÚ d¡ŠgrZ (Q>rH$m) H$m CËnmXZ H¡$go {H$`m OmVm h¡ ?
(I) ImÚ d¡ŠgrZ Ho$ OrdmUw Ûmam CËnm{XV nwZ`m}JO d¡ŠgrZ H$s Anojm A{YH$ bm^àX
h¢ & {H$Ýht VrZ bm^m| H$s gyMr ~ZmBE &
Explain how the desired genes are introduced in plants using vector
mediated gene transfer. Draw diagram. How can one differentiate
between the transgenic and non-transgenic plant ?
OR
(a) How are edible vaccines produced ?
(b) Edible vaccines have advantages over recombinant vaccines
produced by bacteria. List any three advantages. 2+3=5

28. (H$) nmofH$-agm`Z (Ý`yQ´>mñ`y{Q>H$b) àmoQ>rZ Š`m h¢ ?
(I) Xhr H$mo àmo~m`mo{Q>H$ H$s Vah BñVo_mb {H$`m OmVm h¡ & Š`m| ?
(J) nZraOb àmoQ>rZ go ~hþV-go amoJm| H$m BbmO {H$`m Om gH$Vm h¡ & ì`m»`m H$s{OE &
(K) ImÚ nXmWmªo H$s {H$g àUmbr _| nZraOb àmoQ>rZ H$m Ob-Am~§YZ (~mB§qS>J) JwU
BñVo_mb hmoVm h¡ ? 2+1+1+1=5
(a) What are nutraceutical proteins ?
(b) Curd has been used as a pro-biotic. Why ?
(c) Whey protein can treat a spectrum of diseases. Explain.
(d) In which food system is the water binding property of whey protein
used ?
99 8