CBSE Class 12 Question Paper 2022 Biotechnology (Solved)

SET-4
Series %BAB% àíZ-nÌ H$moS>
Q.P. Code 99
amob Z§. narjmWu àíZ-nÌ H$moS> H$mo CÎma-nwpñVH$m Ho$
Roll No. _wI-n¥ð >na Adí` {bIo§ &
Candidates must write the Q.P. Code on
the title page of the answer-book.

H¥$n`m Om±M H$a b| {H$ Bg àíZ-nÌ _o§ _w{ÐV n¥ð> 7 h¢ &
àíZ-nÌ _| Xm{hZo hmW H$s Amoa {XE JE àíZ-nÌ H$moS H$mo narjmWu CÎma-nwpñVH$m Ho$ _wI-n¥>ð> na
{bI| &
H¥$n`m Om±M H$a b| {H$ Bg àíZ-nÌ _| >13 àíZ h¢ &
H¥$n`m àíZ H$m CÎma {bIZm ewê$ H$aZo go nhbo, CÎma-nwpñVH$m _| àíZ H$m H«$_m§H$ Adí`
{bI| &
Bg àíZ-nÌ 15 {_ZQ >H$m g_` {X`m J`m h¡ & àíZ-
10.15 ~Oo {H$`m OmEJm & 10.15 ~Oo go 10.30 ~Oo VH$ N>mÌ Ho$db àíZ-
Bg Ad{Y Ho$ Xm¡amZ do CÎma-nwpñVH$m na H$moB© CÎma Zht {bI|Jo &
Please check that this question paper contains 7 printed pages.
Q.P. Code given on the right hand side of the question paper should be written
on the title page of the answer-book by the candidate.
Please check that this question paper contains 13 questions.
Please write down the serial number of the question in the
answer-book before attempting it.
15 minute time has been allotted to read this question paper. The question
paper will be distributed at 10.15 a.m. From 10.15 a.m. to 10.30 a.m., the
students will read the question paper only and will not write any answer on
the answer-book during this period.

O¡d-àm¡Úmo{JH$s
BIOTECHNOLOGY

:2 : 35
Time allowed : 2 hours Maximum Marks : 35

99 Page 1 P.T.O.

:
(i) 13
(ii)
(iii) 6 2 6
3 1 5
(iv)

IÊS> H$
1. t-PA (D$VH$ ßb¡pµÁ_ZmoOoZ g{H«$`H$) H$s {H«$`m H$mo Xem©Zo Ho$ {bE EH$ àdmh AmaoI
~ZmBE & 2
2. \o$ZZ (\$moq_J) go gyú_-O¡{dH$s àH«$_m| _| g_ñ`mE± h¢ ? 2
3. {ZåZ{b{IV S>oQ>m~og nwZ: ¡? 2
(H$)
(I) µ Oa
4. (H$) \$bm| H$mo Xÿgar OJh ñWmZm§V[aV H$aZo
Ho$ àH«$_ H$mo {H$g àH$ma Yr_m {H$`m OmVm h¡ ? 2
AWdm
(I) AO¡d à{V~b (VZmd) n[apñW{V`m| Ho$ AZwê$n nm¡Ym| _| AZwH$y bZ H¡$go hmoVm h¡ ? 2

5. (H$) JmoëS>Z Mmdb h¡ ? Bgo {H$g àH$ma {dH${gV {H$`m J`m h¡ ? 2
AWdm
(I) H$mog§H$a (gmB{~«S²>g h¢ ? Eogo {H$Ýht Xmo H$mo{eH$mÐì`r` (gmBQ>moßb¡pµÁ_H$)
A{^bjUm| Ho$ Zm_ {b{IE {OÝh| H$mog§H$a (gmB{~«S>m|) Ûmam EH$ nmXn ñnrerµO go
Xÿgar ñnrerµO _| ñWmZm§V[aV {H$`m Om gH$Vm h¡ & 2
6. gr{_V VWm A{daV (gVV) H$mo{eH$m bmBZm| Ho$ ~rM _hÎdnyU© {^ÞVmAm| H$s gyMr ~ZmBE & 2
IÊS> I
7. (H$) g§dY©Z _| ñVZYmar H$mo{eH$mAm| Ho$ Or{dV ahZo Ho$ {bE pH {d{Z`_Z
_hÎdnyU© h¡ ? Xmo H$maU {b{IE &
(I) ~mBH$m~m©oZoQ> CO2 ~\$a V§Ì {H$g àH$ma H$m`© H$aVm h¡ ? 3
8. RefSeq ? BgHo$ H$moB© Xmo _hÎd {b{IE & 3
99 Page 2

General Instructions :
(i) This question paper contains 13 questions. All questions are compulsory.
(ii) This question paper has three sections Section A, B and C.
(iii) Section A contains 6 questions of 2 marks each. Section B contains
6 questions of 3 marks each and Section C has 1 case-based question of
5 marks.
(iv) There is no overall choice. However, internal choices have been provided in
some questions. Only one of the choices in such questions have to be
attempted.
SECTION A
1. Construct a flowchart showing the action of t-PA (tissue plasminogen
activator). 2
2. Why can foaming cause problems in microbiological processes ? 2
3. What information can be obtained from the following Database retrieval
tools ? 2
(a) Locus link
(b) Taxonomy browser
4. (a) Ripening of fruits has been slowed down to prevent their spoilage
during transportation. How ? 2
OR
(b) Plants have adapted to cope with abiotic stress conditions. How ? 2
5. (a) 2
OR
(b) What are Cybrids ? Name any two cytoplasmic traits that can be
transferred using Cybrids, from one plant species to another. 2
6. Enlist important differences between finite and continuous cell lines. 2
SECTION B
7. (a) Why is regulation of pH important for survival of mammalian cells
in culture ? Write two reasons.
(b) How does bicarbonate CO2 buffering system work ? 3
8. What is RefSeq ? Give its significance. (any 2 points) 3

99 Page 3 P.T.O.

9. (H$) gyú_Ordr g§dY©Z Ho$ {bE Cn`moJ {H$E OmZo dmbo _mÜ`_ _| noßQ>moZ, _m§g H$m AH©$,
I_ra H$m AH d O¡go {d{^Þ KQ>H$ hmoVo h¢ &
`h g§dY© _mÜ`_ EH$ O{Q>b _mÜ`_ h¡ & `m| ?
(I) {H$gr ^r nmofH$ g§dY© _mÜ`_ _| EJma H$m {ZdoeZ ({_bm`m) {H$`m OmVm h¡ ?
(J) EH$ Am° _mÜ`_ Ho$ AZwd©arH$aU Ho$ {bE H$m¡Z-H$m¡Z gr n[apñW{V`m±
Amdí`H$ h¢ ? 3

10. (H$) CO2 C ~oQ>a) gOrd H$mo{eH$mAm| H$s Am§V[aH$ n[apñW{V`m| Ho$ AZwê$n
Cn`wº$ n`m©daUr` n[apñW{V`m± àXmZ H$aVo h¢, Bg{bE OÝVw H$mo{eH$m g§dY© H$mo
CO2 Cî_m{`Ì _| aIm OmVm h¡ & H¡$go ? 3
AWdm
(I) OÝVw H$mo{eH$m g§dY©Z _| gra_ EH$ _hÎdnyU© KQ>H$ h¡ & H$WZ Ho$ g_W©Z _| Xmo
g_w{MV H$maU Xr{OE & g§dY© _mÜ`_ _| à{VO¡{dH$m| H$mo {_{lV ({Zdo{eV
{H$`m OmVm h¡ ? 3

11. µ\$gb _| AZmdí`H$ namJU Ho$ AdamoYZ hoVw EH$ {H$gmZ {dnw§gZ àH«$_ go {H$g
àH$ma ~M gH$Vm h¡ ?
gw{ZpíMV {H$`m Om gH$Vm h¡ ? 3

12. H¥${Ì_ ~rO H$m Zm_m§ ? 3
(Ho$db ÑpîQ>~m{YV narjm{W©`m| Ho$ {bE)
? ? 3

IÊS> J
13. (H$) gyú_Ordr g§dY©Z :
AË`{YH$ Cn`moJr `m¡{JH$m| (nXmWmªo) Ho$ CËnmXZ _| gyú_Ordr g§dY©Zm| Ho$ `moJXmZ
H$s Agr_ g§^mdZmE± h¢ & BZ g§dY©Zm| H$m Cn`moJ {d{^Þ CnmnM`Om|
(_oQ>m~mobmBQ>m|) Ho$ CËnmXZ Ho$ {bE {H$`m Om gH$Vm h¡ : E§µOmB_, ñQ>oam°`S>,
à{VO¡{dH$, CnMmar àmoQ>rZ, gyú_Ordr {ZjmbZ (brqMJ), O¡d-AnKQ>Zr` An{eîQ>
CnMma, BË`m{X BgHo$ Hw$N> CXmhaU h¢ &
(i) EH$ Eogm CXmhaU Xr{OE {Og_| AZwn`moJr {H«$`mYma (g~ñQ´>oQ>) H$mo Cn`moJr
CËnmXm| _| n[ad{V©V H$aZo Ho$ {bE gyú_Ordr CnmnM` H$m Cn`moJ {H$`m OmVm
h¡ & 1
99 Page 4

9. (a) A medium for microbial culture contains components like peptone,
meat extract, yeast extract, casein digest and corn steep liquor.
This culture medium is a complex medium. Why ?
(b) Why is agar added to any nutrient medium ?
(c) What are the conditions for sterilization of culture medium in an
autoclave ? 3

10. (a) Animal cell cultures are placed in CO2 incubators, as they help to
reproduce environmental conditions close to the living cells. How ? 3
OR
(b)
the statement giving two reasons. Why are antibiotics added to
culture medium ? 3

11. How can a farmer eliminate the process of emasculation to prevent
unnecessary pollination in his maize crop ? Can normal pollen formation
be ensured at the onset of breeding seasons ? 3

12. Draw a labelled diagram of an artificial seed. Why are these seeds
produced ? 3
(For Visually Impaired Candidates Only)

What are artificial seeds ? Why are they produced ? 3

SECTION C

13. (a) Microbial Cultures :
Microbial cultures have immense potential for production of very
useful compounds. These cultures can be exploited mainly to
produce different metabolites which include enzymes, steroids,
antibiotics, therapeutic proteins, microbial leaching, biodegradable
waste treatment, etc.
(i) Give an example where microbial metabolism has been used
to convert unsuitable substrates to useful products. 1

99 Page 5 P.T.O.

(ii) DSP H$s n[a^mfm {b{IE & EH$ DSP àmoQ>moH$m°b _| H$_-go-H$_ MaUm| Ho$
? 1
(iii) ? 1
(iv) {H$g Ord H$mo GRAS H$hm OmVm h¡ ? 1
(v) gyú_Ordr àm¡Úmo{JH$s _| Xmo O¡d-gwajm _wÔm| H$m g§{jßV CëboI H$s{OE & 1

AWdm
(I) gVV (A{daV) g§dY© :
gyú_Ordr d¥{Õ H$s gVV (A{daV) Amny{V© Ho$ {bE, A{daV g§dY©Z H$m Cn`moJ
{H$`m OmVm h¡ & g§dY© _mÜ`_ H$mo Bg àH$ma g§`mo{OV ({S>µOmBZ) {H$`m OmVm h¡ {H$
nmo
bJmVma S>mbo OmVo h¢ VWm g§dY© H$mo g_`-g_` na {ZH$mbm OmVm h¡ & Bg àH$ma
g§dY©Z go ñWmB© CËnmX JwUdÎmm VWm à{V BH$mB© Am`VZ _| A{YH$ CËnmXZ àmßV
H$aZo _| ghm`Vm {_bVr h¡, Bg{bE Bg VH$ZrH$ H$m Cn`moJ gyú_Ordr ~m`mo_mg
VWm CZHo$ CnmnM`Om| Ho$ CËnmXZ _| {H$`m Om ahm h¡ &
(i) Q>{~©S>moñQ>oQ> VWm Ho$_moñQ>oQ> _| A§Va ñnîQ> H$s{OE & 2
(ii) Bg J«m\$ _| {H$g àH$ma H$m gyú_Ordr g§dY© {XIm`m J`m h¡ ? 1

g_`
(Ho$db ÑpîQ>~m{YV narjm{W©`m| Ho$ {bE ^mJ (ii) H$m {dH$ën)
KmZ h¡ ? 1
(iii) g§dY© nmÌ Ho$ Am`VZ Ho$ g§X^© _| A{daV g§dY© \o$S>-KmZ g§dY© go {H$g
à«H$ma {^Þ h¡ ? {H$g àH$ma H$s {d{Y _| gyú_Ordr g§dY© H$mo EH$ {dñVm[aV
bm°J ê$n _| AWdm pñWa AdñWm _| ~ZmE aIm Om gH$Vm h¡ ? 1+1

99 Page 6

(ii) Define DSP. Why is it advisable to use less number of steps
in a DSP protocol ? 1
(iii) What are excipients ? 1
(iv) Which organisms are referred to as GRAS ? 1
(v) Briefly mention two biosafety issues specific in microbial
technology. 1
OR
(b) Continuous Culture :
To get a continuous supply of microbial growth, continuous culture
is used. The growth medium is designed in such a way that one of
the nutrients is in limited quantity. Nutrients are continually
added to the bioreactor and spent culture broth is removed at the
same time. This culture helps to obtain stable product quality and
higher productivity per unit volume, so this technique is widely
used for production of both microbial biomass as well as their
metabolites.
(i) Differentiate between turbidostat and chemostat. 2
(ii) Which type of microbial culture is shown in this graph ? 1

Time
(For Visually Impaired Candidates Only in lieu (ii))
What is Batch Culture ? 1
(iii) How is continuous culture different from Fed-Batch culture
in terms of volume in the culture vessel ? In which type of
method can microbial culture be maintained in an extended
log or stationary phase ? 1+1

99 Page 7 P.T.O.