Manipur Board Class 12 Syllabus 2027 Biotechnology

FOR MANIPUR CLASS 12 PREPARATION

Manipur Board Class 12
Syllabus 2027
Biotechnology
EXAM YEAR TYPE SUBJECT

Manipur Class 12 2027 Syllabus Biotechnology

Notes · Sample Papers · Previous Year Papers · Mock Tests

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BIOTECHNOLOGY
An unprecedented growth of human knowledge in the field of Biological Sciences coupled
with equally significatn developments in the field of technology have brought significant changes
into existing social and economic systems. The emerging field of Biotechnology is likely to further
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enhance the applications of Science and Technology in the service of human welfare. Modern
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Biotechnology processes encompass a wide range of new products such as antibiotics, vaccines,
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monoclonal antibodies and many more. Furthermore, developments in recombinant DNA technology
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have yielded numerous new useful products in the fields of healthcare and agriculture.
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l present syllabus takes care of all these aspects. Due emphasis has been laid on familiarizing
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the learners with the fundamental concepts, basic techniques and their applications. It is expected that
the knowledge gained through the study of different topics and the skills acquired through the prescribed
practical work will make the learners competent to meet the challenges of academic as well as
professional courses after studying the subject at senior secondary stage.

OBJECTIVES

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The broad objectives of teaching Biotechnology at senior secondary level are :
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To help the learners know and understand basic facts and concepts in the subject at
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s basic processes and basic techniques used in
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 To expose the students toldifferent
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 To familiarize the learners to understand the relationship of the subject to health, nutrition,
environment, agriculture and industry etc.
 To develop conceptual competence in the learners so as to cope up with professional
courses in future career.
 To acquaint students with different applications of Biotechnology in everyday life. To
develop an interest in students to study biotechnology as a discipline.
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BIOTECHNOLOGY
THEORY
COURSE STRUCTURE
CLASS - XII
One Paper Time : 3 Hours 70 Marks
Unit Contents Marks
I. Protein and Gene Manipulation
Chapter I Protein Structure and Engineering 15
Chapter II Recombinant DNA Technology 15
Chapter III Genomics and Bioinformatis 10
II. Cell Culture Technology
Chapter I Microbial Cultural and Application 10
Chapter II Plant Cell Culture and Application 10
Chapter III Animal Cell Culture and Application 10
Total 70

Unit-I : Protien and Gene Manipulation Marks 40
Chapter I: Protein Structure and Engineering 15 Marks
Introduction to the world of Proteins
3-D Shape of Proteins
Structure Function relationship in Proteins
Purification of Proteins
Characterization of Proteins
Protein based products
Designing Proteins
Proteomics
Chapter II: Recombinant DNA Technology 15 Marks
Introduction
Tools of DNA Technology
Making Recombinant DNA
DNA Library
Introduction of Recombinant DNA into host cells
Identification of recombinants
Polymerase Chains Reaction (PCR)
DNA Probes
Hybridization Techniques
DNA Sequencing
Site-directed mutagenesis

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Chapter III: Genomics and Bioinformatics 10 Marks
Introduction
Genome Sequencing Projects
Gene Production and counting
Genome similarity, SNP’s and comparative genomics
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Functional Genomics
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History of Bioinformatics
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Sequences and Nomenclature
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Information Sources
Analysis using Bioinformatics tools. ag
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Unit-II : Marks 30
Chapter I: Microbial Culture and Applications 10 Marks
Introduction
Microbial Culture Techniques
Measurement and Kinetics of microbial Growth
Scale up of microbial process
Isolation of microbial products m
Strain isolation and Improvement
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Applications of microbial culture technology
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Bioethics in microbial technology
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Chapter II: Plant Cell Culture and 10 Marks
Introduction
Cell and Tissue Culture Techniques
Applications of Cell and Tissue Culture
Gene Transfer Methods in Plants
Transgenic Plants with Beneficial Traits
Diagnostics in Agriculture and Molecular Breeding
Bioethics in Plant Genetic Engineering.
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Chapter III: Animal Cell Culture and Applications
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m Introduction
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a Scale-up of Animal Culture Process
Applications of Animal Cell Culture
Stem Cell Technology
Bioethics of Genetic Engineering in Animals

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PRACTICALS
Note : Every student will be required to do the following experiments during the academic session.
1. Isolation of bacterial plasmid DNA and its detection by gel electrophoresis.
2. Restriction digestion of plasmid DNA and its analysis by gel electrophoresis
3. Bacterial transformation using any plasmid.
4. Data retrieval and data base search using internet site NCBI.
5. Download a DNA and protein sequence from internet, analyse and comment on it.
6. Cell viability assay (using Evans blue Stain)
7. Determination of blood groups.
8. Estimation of DNA
9. Ion-exchange chromatography for proteins.
10. Reading of a DNA sequencing gel and arrive at the sequence.
11. Estimation of blood glucose by enzymatic method (GOD/POD)
12. Project work.

Scheme of Evaluation :
Time : 3 Hours Max. Marks 30
The scheme of evaluation at the end of session will be as under :
A. Two experiments : 6+6 (only one computer based practical)
Practical record : 04
Viva on Practicals : 04
B. Project work :
Write up : 05
Viva on project : 05
Total 30

Recommended Books :
1. A Textbook of Biotechnology-Class XI : published by CBSE, New Delhi.
2. A Laboratory Manual of Biotechnology-Class XI : Published by CBSE, New Delhi.
3. A Textbook of Biotechnology-Class XII. : published by CBSE, New Delhi.
4. A Laboratory Manual of Biotechnology-Class XII : Published by CBSE, New Delhi.

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FOR THE ACADEMIC SESSION 2024-25

DESIGN OF
QUESTION PAPER
Subject : BIOTECHNOLOGY
Paper : Theory
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Class : XII

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Time
:
:
70
3 Hours
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WEIGHTAGE TO OBJECTIVES:
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Objectives
Knowledge (K)
Marks
14
Percentage
20
I Understanding (U) 32 46
Application (A) 21 30
Skill (S) 3 4
Total : 70 100
WEIGHTAGE TO FORM OF QUESTIONS:
Form of Questions No. of Question Time (in minute) Marks Percentage
Essay/Long Answer (E/LA) 3 60 15 21
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Short Answer (SA-I) 6 36 18 26
II Short Answer (SA-II) 10 40 20 29
Very Short Answer (VSA) 10 e m 30 10 14
MCQ
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7 as 14 7 10

WEIGHTAGE TO CONTENT:
Total:
ag36 180 70 100

UNIT/CONTENTS: Marks Percentage
Protein & Gene Manipulation
I Protein Structure & Engineering 15 22
1 II Recombinant DNA Technology 15 22
III Genomics & Bioinformatics 10 14
III
Cell Culture Technology
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I Microbial Culture & Application 10 14
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II Plant Cell Culture & Application 10
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14
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las 14

las IV SCHEME OF SECTIONS : Nil
Total : 70
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ag V SCHEME OF OPTIONS : Internal option may be given in Essay Type Question & SA-I.
VI DIFFICULTY LEVEL :
Difficult : 30%
Average : 50%
Easy : 20%

Abbreviation : K(Knowledge), U(Understanding ), C(Comprehension), Exp.(Expression), Skill(S),
E(Essay Type), SA (Short Answer Type), VSA (Very Short Answer Type),
MCQ(Multiple Choice Question)
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FROM THE ACADEMIC SESSION 2025-26

DESIGN OF
QUESTION PAPER
Subject : BIOTECHNOLOGY
Paper : Theory
Class : XII
Full Mark : 70
Time : 3 Hours

WEIGHTAGE TO OBJECTIVES:
Objectives Marks Percentage
Knowledge (K) 10 15
I Understanding (U) 35 50
Application (A) 21 30
Skill (S) 4 5
Total : 70 100
WEIGHTAGE TO FORM OF QUESTIONS:
Form of Questions No. of Question Time (in minute) Marks Percentage
Essay/Long Answer (E/LA) 3 60 15 21
Short Answer (SA-I) 6 37 18 26
II Short Answer (SA-II) 10 40 20 29
Very Short Answer (VSA) 7 21 7 10
MCQ 10 22 10 14
Total: 36 180 70 100
WEIGHTAGE TO CONTENT:
UNIT/CONTENTS: Marks Percentage
Protein & Gene Manipulation
I Protein Structure & Engineering 15 21
1 II Recombinant DNA Technology 15 21
III Genomics & Bioinformatics 10 14
III
Cell Culture Technology
I Microbial Culture & Application 10 15
2
II Plant Cell Culture & Application 10 15
III Animal Cell Culture & Application 10 14
Total : 70 100
IV SCHEME OF SECTIONS : Nil
V SCHEME OF OPTIONS : i) Internal option will be given in Essay Type Question
ii) Internal option will be given in three questions of SA-I
including one case study based question.
VI DIFFICULTY LEVEL :
Difficult : 35% of the total marks
Average : 50% of the total marks
Easy : 15% of the total marks

Abbreviation : K(Knowledge), U(Understanding), A(Application), Skill(S), E/LA (Essay/Long
Answer Type), SA (Short Answer Type), VSA (Very Short Answer Type),
MCQ(Multiple Choice Question)

NOTE- (i) Two questions out of 10 (ten) questions of MCQ will be assertion & reason type question.
(ii) Only one question of SA-I will be Case Study based question.
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